AB289847

DPPH Assay Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(1 Publication)

DPPH assay kit ab289847 is a DPPH antioxidant / radical scavenging assay used to quantify antioxidant capacity. The assay requires a single 10-min incubation at room temperature. Readout on any colorimetric (517 nm) plate reader.

5 Images

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

FuncS

Supplier Data

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

DPPH Assay Kit (Colorimetric).

DPPH assay using green tea and kiwi fruit samples.

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

FuncS

Supplier Data

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

DPPH Assay Kit (Colorimetric).

Linear relationship between DPPH concentrations and Absorbance.

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

FuncS

Supplier Data

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

DPPH Assay Kit (Colorimetric).

Trolox Standard Curve (linear range of inhibition changes after Trolox treatment under 300 μM DPPH concentration).

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

FuncS

Supplier Data

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

DPPH Assay Kit (Colorimetric).

Logistic fitting including the dilution factor for calculating EC₅₀.

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

FuncS

Supplier Data

Functional Studies - DPPH Assay Kit (Colorimetric) (AB289847)

DPPH Assay Kit (Colorimetric).

Logistic fitting including the dilution factor for calculating EC₅₀.

Key facts

Detection method

Colorimetric

Sample types

Food samples, Serum, Other biological fluids

Results type

Quantitative

Assay Platform

Microplate

style

left-column

style

left-column

Product details

DPPH assay kit ab289847 is used to quantify the antioxidant capacity of samples including foods, beverages, biological fluids (e.g. human serum), etc. The DPPH assay is also referred to as the DPPH antioxidant assay and the DPPH radical scavenging assay.

DPPH assay principle
The DPPH assay is based on the stable free radical 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). In the assay, DPPH, which is deep blue in color, is reduced by reaction with antioxidants to form 2,2-diphenyl-1-picrylhydrazine (DPPH-H), which is pale yellow in color. This color change is measured by a decrease in absorbance at 517 nm, corresponding to the blue color of DPPH. The decrease in absorbance is proportional to the antioxidant capacity of the sample.

The synthetic antioxidant, Trolox is included in this assay kit and is used to standardize the sample antioxidant capacity relative to Trolox (Trolox Equivalents Antioxidant Capacity, TEAC).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab289847-100Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"DPPH Assay Buffer", "number":"AB289847-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Trolox Standard", "number":"AB289847-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"DPPH Probe", "number":"AB289847-CMP01", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Please refer to protocols

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPPH also known as 11-diphenyl-2-picrylhydrazyl is a stable free radical. It has a molecular mass of 394.32 g/mol. Scientists often use it in its radical form which presents as a deep violet color. This color changes upon reduction making it an excellent marker for antioxidant activity. While DPPH is not a biological protein it is extensively used in vitro to evaluate the free radical scavenging activities of antioxidants.

Biological function summary

In vitro antioxidant assays such as the DPPH assay measure how substances can neutralize free radicals. These assays use the DPPH molecule as a tool to quantify antioxidant activity in different samples. The DPPH assay protocol involves observing the change in color from deep violet to a pale yellow as antioxidants in the sample reduce DPPH radicals to non-radical DPPH-H form. The degree of color change reflects the sample's antioxidant capacity.

Pathways

DPPH assays help in understanding oxidative stress pathways by measuring the antioxidants' ability to counteract free radicals. These assays relate to pathways involving the oxidative stress response such as the Nrf2 signaling pathway which modulates the expression of antioxidant proteins. Although the DPPH radical itself does not participate in biological pathways its use in assays helps track activities alongside key proteins like glutathione.

Oxidative stress plays a significant role in chronic inflammation and cardiovascular diseases. Antioxidant assessments using the DPPH test help evaluate how certain compounds might prevent or mitigate these conditions by neutralizing harmful free radicals. The assay indirectly links to disease mechanisms influenced by proteins like NADPH oxidase which generates oxidative stress associated with these diseases.

style

left-column

Product protocols

style

left-column

Target data

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38398866

2024

Bulbils of Aerial Yam Attenuate Ethanol-Induced Hepatotoxicity in HepG2 Cells through Inhibition of Oxidative Stress by Activation of the Nuclear Factor Erythroid-2-Related Factor 2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Kwon Yang,Tsendsuren Tungalag,Hyung-Sub Kang

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

en

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com