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AB133406

ECL Substrate Kit (High Sensitivity)

5

(5 Reviews)

|

(58 Publications)

ECL Substrate Kit (High Sensitivity) (ab133406) is an enhanced chemiluminescent substrate designed for the detection of proteins with 23 pg-187 ng of protein per band.

- Long lasting, highly sensitivity substrate- strong signal with very low background
- Use for film imaging and compatible with CCD imaging
- Available in different sizes to fit your experimental needs
2 Images
Western blot - ECL Substrate Kit (High Sensitivity) (AB133406)
  • WB

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Western blot - ECL Substrate Kit (High Sensitivity) (AB133406)

Western blot comparison 1/25000 anti-Transferrin primary antibody, 1/50000 secondary antibody, 20 second exposure.

Anti-Transferrin antibody (ab1223) at 1/25000 dilution. Lanes 1-14 Transferrin protein (ab91435), Loading dilution (Lanes 1-8) : 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19μg protein.

Secondary

HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.

Exposure time : 20 seconds

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Western blot - ECL Substrate Kit (High Sensitivity) (AB133406)
  • WB

Supplier Data

Western blot - ECL Substrate Kit (High Sensitivity) (AB133406)

Western Blot 1/25000 primary antibody, 1/5000 secondary antibody, 20 second exposure.

Each lane contains the following amount of COX2 recombinant protein (ab58868) : 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) at 1/25000 dilution and with a rabbit secondary (ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Detect.

Exposure time : 20 seconds

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Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.

Our ECL kits include our popular ECL Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
- this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band

Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.

This product was previously called Optiblot ECL Detect Kit (23pg-187ng).

Use:
- 200ml kit for 2000cm2 membrane
- 500ml kit for 5000cm2 membrane

The primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (58)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 21:188-199 PubMed38164346

2024

IL1A regulates the inflammation in gout through the Toll-like receptors pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meirong Ling,Jiaqi Gan,Mengting Hu,Fei Pan,Mei Liu

Oncology letters 27:8 PubMed38028180

2023

Effect of IFN‑γ encapsulated liposomes on major signal transduction pathways in the lymphocytes of patients with lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maysa Alhawamdeh,Belal Almajali,Wafa Hourani,Hamid Ali Nagi Al-Jamal,Abdullah Saleh Al-Wajeeh,Nesrin Riad Mwafi,Yousef Al-Hajaya,Hanan Kamel M Saad,Diana Anderson,Mahmoud Odeh,Ibraheam A Tarawneh

Oncology letters 26:492 PubMed37854860

2023

MicroRNA‑27a‑3p regulates the proliferation and chemotaxis of pulmonary macrophages in non‑small cell lung carcinoma tissues through CXCL2.

Applications

Unspecified application

Species

Unspecified reactive species

Congying Zhai,Baoliang Liu,Fanggong Kan,Shuhui Zhai,Ronghua Zhang

Acta biochimica Polonica 70:261-269 PubMed37331014

2023

β-catenin promotes resistance to trastuzumab in breast cancer cells through enhancing interaction between HER2 and SRC.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Hao,Jialu Zheng,Xiaoqin Yu,Zhixin Li,Guanghui Ren

PLoS biology 21:e3002098 PubMed37126497

2023

A single N-terminal amino acid determines the distinct roles of histones H3 and H3.3 in the Drosophila male germline stem cell lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Chinmayi Chandrasekhara,Rajesh Ranjan,Jennifer A Urban,Brendon E M Davis,Wai Lim Ku,Jonathan Snedeker,Keji Zhao,Xin Chen

Molecular psychiatry 28:2382-2397 PubMed36991133

2023

Elevated ROS levels during the early development of Angelman syndrome alter the apoptotic capacity of the developing neural precursor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lilach Simchi,Pooja Kri Gupta,Yonatan Feuermann,Hanoch Kaphzan

Histology and histopathology 38:1429-1441 PubMed36852950

2023

Hsa_circ_0070440 promotes lung adenocarcinoma progression by SLC7A11-mediated-ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhao,Qichen Cui,Jian Shen,Weihong Shen,Yuan Weng

Open medicine (Warsaw, Poland) 18:20220620 PubMed36820068

2023

SIRT1/APE1 promotes the viability of gastric cancer cells by inhibiting p53 to suppress ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huijin Zhao,Yuanyi Ding,Lan Zhang

Life science alliance 6: PubMed36623871

2023

Linking medicinal cannabis to autotaxin-lysophosphatidic acid signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias C Eymery,Andrew A McCarthy,Jens Hausmann

The Journal of dermatology 50:472-484 PubMed36412048

2022

GATA3 ameliorates melanocyte injuries in vitiligo through SIRT3-mediated HMGB1 deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Juan Nie,Bao-Zhen Hao,Ben-Li Zhang,Yuan-Yuan Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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