ECL Substrate Kit (High Sensitivity)

Product details

High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.

Our ECL kits include our popular ECL Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
- this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band

Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.

This product was previously called Optiblot ECL Detect Kit (23pg-187ng).

Use:
- 200ml kit for 2000cm² membrane
- 500ml kit for 5000cm² membrane

The primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.