High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.
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High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.
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High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.
Our ECL kits include our popular ECL Substrate Kit ECL Western Blotting Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
- this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ECL Substrate Kit (Very High Sensitivity) ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ECL Substrate Kit (Ultra High Sensitivity) ab133409 to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
This product was previously called Optiblot ECL Detect Kit (23pg-187ng).
Use:
- 200ml kit for 2000cm2 membrane
- 500ml kit for 5000cm2 membrane
The primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Western blot comparison 1/25000 anti-Transferrin primary antibody, 1/50000 secondary antibody, 20 second exposure.
Anti-Transferrin antibody (ab1223) at 1/25000 dilution. Lanes 1-14 Transferrin protein (Native Human Transferrin protein ab91435), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19μg protein.
Secondary
HRP conjugated polyclonal to Rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.
Exposure time: 20 seconds
Western Blot 1/25000 primary antibody, 1/5000 secondary antibody, 20 second exposure.
Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (Anti-COX2 / Cyclooxygenase 2 antibody ab15191) at 1/25000 dilution and with a rabbit secondary (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Detect.
Exposure time: 20 seconds
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