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Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.

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Images

Western blot - ECL Substrate Kit (Very High Sensitivity) (AB133408), expandable thumbnail
  • Western blot - ECL Substrate Kit (Very High Sensitivity) (AB133408), expandable thumbnail

Publications

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

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What's included?

20 mL
Components
Luminol/enhancer solution
1 x 10 mL
Peroxide Chemiluminescent Detection Reagent
1 x 10 mL

Recommended products

Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging. It is designed for the detection of proteins from 4.6pg to 4.7ng of protein per band.

ECL Substrate Kit ab133408 produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from the ECL substrate is linear with respect to protein amount over a broad range of concentrations, displaying no substrate depletion at high protein loads, allowing the user to take full advantage of the linear range of the CCD detection method.

The ECL substrate is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative.

Our ECL kits include our popular ECL Substrate Kit ECL Western Blotting Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
- High Sensitivity ECL Substrate Kit ECL Substrate Kit (High Sensitivity) ab133406 to detect 23pg-187ng of protein per band
- this kit (Very High Sensitivity ECL Substrate Kit ab133408) to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ECL Substrate Kit (Ultra High Sensitivity) ab133409 to detect 1.2pg-2ng of protein per band

Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.

This product was previously called Optiblot ECL Max Detect Kit (4.6pg-4.7ng).

Use:
- 100ml kit for 1000cm2 membrane
- 200ml kit for 2000cm2 membrane

The primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - ECL Substrate Kit (Very High Sensitivity) (ab133408), expandable thumbnail

    Western blot - ECL Substrate Kit (Very High Sensitivity) (ab133408)

    Western Blot 1/50000 primary antibody, 1/5000 secondary antibody, 10 second exposure.

    Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (Anti-COX2 / Cyclooxygenase 2 antibody ab15191) at 1/50000 dilution and with a rabbit secondary (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Max Detect.

    Exposure time: 10 seconds

  • Western blot - ECL Substrate Kit (Very High Sensitivity) (ab133408), expandable thumbnail

    Western blot - ECL Substrate Kit (Very High Sensitivity) (ab133408)

    Western Blot 1/25000 primary antibody, 1/5000 secondary antibody, 10 second exposure.

    Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (Anti-COX2 / Cyclooxygenase 2 antibody ab15191) at 1/25000 dilution and with a rabbit secondary (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Max Detect.

    Exposure time: 10 seconds

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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