ECL Western Blotting Substrate Kit

The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.

Our ECL kits include this kit ab65623 and our high sensitivity ECL substrate kits:
- High Sensitivity ECL Substrate Kit ab133406 to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band 

Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.

This product is manufactured by BioVision, an Abcam company and was previously called K820 ECL Western Blotting Substrate Kit. K820-500 is the same size as the 500 test size of ab65623.

Reagents provided are sufficient for 400 cm² of membrane.