EdU Assay / EdU Staining Proliferation Kit (iFluor 488)

Product details

How the assay works

The EdU Assay / EdU Staining Proliferation Kit (iFluor 488) detects cell proliferation by measuring the amount of 5-ethynyl-2'-deoxyuridine (EdU) incorporated into DNA during the S-phase of the cell cycle. The iFluor 488 dye (Ex/Em: 491/520 nm) has spectral properties almost identical to those of FITC and alternative green fluorophores.

EdU staining protocol summary (wash cells between each step):

- Add EdU solution to cells to be stained
- Incubate cells for 2-4 hrs under optimal growth conditions
- Add fixative solution and incubate for 15 min
- Add permeabilization buffer and incubate for 15/20 min
- Add reaction mix to fluorescently label EdU and incubate for 30 min
- Analyze with flow cytometer / fluorescence microscope

Related and recommended products

See an alternative EdU Assay:
EdU Assay / EdU Staining Proliferation Kit (iFluor 647) ab222421

Previously called EdU Proliferation Assay Kit (iFluor 488). The most accurate method to measure DNA proliferation is by directly measuring DNA synthesis. The most common method for this uses antibody-based detection of the nucleoside analog bromo-deoxyuridine (BrdU). EdU (5-ethynyl-2’-deoxyuridine), a thymidine analog that is an alternative to BrdU, is also used in DNA proliferation assays that are simpler and faster than the BrdU assay. NB: EdU is also available as free molecule as ab146186 (EdU). In EdU staining, EdU is incorporated into newly synthesized DNA by cells within a sample. A fluorescent azide, such as iFluor-488, is then added. The fluorescent azide is small enough to diffuse freely through native tissues and DNA, and it covalently cross-links to the EdU in a 'click' chemistry reaction. The main advantages of EdU staining over using BrdU are: - no harsh DNA hydrolysis / DNA denaturing step is required with EdU staining (unlike in the BrdU assay where it is used to give the BrdU antibody access to BrdU within the DNA) - EdU staining is faster, and has less steps, than BrdU staining

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.