The EdU DNA Synthesis Monitoring Kit ab287859 (previously known as EZClick EdU DNA Synthesis Monitoring Kit, K946) utilizes a novel approach that relies on incorporation of 5-EdU (5-ethynyl-2'deoxyuridine) as nucleoside analog to thymidine into newly synthesized DNA directly in the cell culture.
Images
Key facts
- Detection method
- Flow cytometry-fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
What's included?
Recommended products
The EdU DNA Synthesis Monitoring Kit ab287859 (previously known as EZClick EdU DNA Synthesis Monitoring Kit, K946) utilizes a novel approach that relies on incorporation of 5-EdU (5-ethynyl-2'deoxyuridine) as nucleoside analog to thymidine into newly synthesized DNA directly in the cell culture.
Key facts
- Detection method
- Flow cytometry-fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
- Assay Platform
- Flow cytometer, Fluorescence microscope
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The EdU DNA Synthesis Monitoring Kit ab287859 (previously known as EZClick EdU DNA Synthesis Monitoring Kit, K946) utilizes a novel approach that relies on incorporation of 5-EdU (5-ethynyl-2’deoxyuridine) as nucleoside analog to thymidine into newly synthesized DNA directly in the cell culture. Incorporation of EdU into genomic DNA in S-phase is detected based on a click reaction between the alkyne moiety of EdU and fluorescent azide. Compared to historically used BrdU, click reaction is carried in mild conditions and flow cytometry/fluorescence microscopy can be used for assessment of proliferating cells in the population. Our kit provides sufficient materials for 100 assays based on the protocol below.
Applications:
- Detection of DNA synthesis in proliferating cells and assessment of cell cycle phase
- Screening for genotoxic compounds and effectors of cell division cycle
- Evaluating effects of anti-cancer drugs and genotoxic agents
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Flow Cytometry - EdU DNA Synthesis Monitoring Kit Flow Cytometry (ab287859)
EZClick EdU DNA Synthesis Monitoring Kit Flow Cytometry.
Analysis of newly synthesized DNA in proliferating cells by Jurkat (106 cells/ ml) cells
Jurkat cells incubated with vehicle (red) or in presence of 100 μg/ml Ganciclovir to suppress DNA biosynthesis (green). Fluorescence measured in FL-2 channel reflects decreased number of proliferating cells treated with Ganciclovir vs. control population
Flow Cytometry - EdU DNA Synthesis Monitoring Kit Flow Cytometry (ab287859)
EZClick EdU DNA Synthesis Monitoring Kit Flow Cytometry 2.
Total DNA content of proliferating cells cultured for 24 h without (green) and with Ganciclovir (red) detected with EZClick Total DNA Stain. Fluorescence was measured in FL-1 channel in the linear mode. Ganciclovir arrests proliferating cells in the S phase of the cell cycle. Fixation and Permeabilization followed by detection with EZClick Fluorescent Azide and counterstaining with EZClick Total DNA Stain was conducted according to the kit protocol.
Fluorescence Microscopy - EdU DNA Synthesis Monitoring Kit Flow Cytometry (ab287859)
EZClick EdU DNA Synthesis Monitoring Kit Fluorescence Microscopy.
Analysis of newly synthesized DNA in proliferating cells by HeLa (105 cells/mL)
Treatment with 10 mM Hydroxyurea (bottom panels) suppressed DNA biosynthesis by blocking DNA replication. Total DNA staining in top and bottom panels clearly confirms that red fluorescence is the result of EdU incorporation during cell proliferation
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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