EdU DNA Synthesis Monitoring Kit Flow Cytometry

• Flow Cyt

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Flow Cytometry - EdU DNA Synthesis Monitoring Kit Flow Cytometry (AB287859)

EZClick EdU DNA Synthesis Monitoring Kit Flow Cytometry 2.

Total DNA content of proliferating cells cultured for 24 h without (green) and with Ganciclovir (red) detected with EZClick Total DNA Stain. Fluorescence was measured in FL-1 channel in the linear mode. Ganciclovir arrests proliferating cells in the S phase of the cell cycle. Fixation and Permeabilization followed by detection with EZClick Fluorescent Azide and counterstaining with EZClick Total DNA Stain was conducted according to the kit protocol.

• Fluorescence Microscopy

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Fluorescence Microscopy - EdU DNA Synthesis Monitoring Kit Flow Cytometry (AB287859)

EZClick EdU DNA Synthesis Monitoring Kit Fluorescence Microscopy.

Analysis of newly synthesized DNA in proliferating cells by HeLa (105 cells/mL)

Treatment with 10 mM Hydroxyurea (bottom panels) suppressed DNA biosynthesis by blocking DNA replication. Total DNA staining in top and bottom panels clearly confirms that red fluorescence is the result of EdU incorporation during cell proliferation

• Flow Cyt

Supplier Data

Flow Cytometry - EdU DNA Synthesis Monitoring Kit Flow Cytometry (AB287859)

EZClick EdU DNA Synthesis Monitoring Kit Flow Cytometry.

Analysis of newly synthesized DNA in proliferating cells by Jurkat (106 cells/ ml) cells

Jurkat cells incubated with vehicle (red) or in presence of 100 μg/ml Ganciclovir to suppress DNA biosynthesis (green). Fluorescence measured in FL-2 channel reflects decreased number of proliferating cells treated with Ganciclovir vs. control population