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Elastic Stain Kit ab150667 uses a modified Verhoeff's Van Gieson / EVG stain to stain elastic fibers / elastin in tissue sections in histology.

- Use for connective tissue fiber analysis
- Suitable for Formalin-Fixed Paraffin-Embedded (FFPE) sections
- Includes ready-to-use solutions of Cresyl Echt Violet, Luxol Fast Blue, Lithium carbonate and 70% alcohol
- Cited in over 40 publications

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Images

Histology - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (AB150667), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (AB150667), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (AB150667), expandable thumbnail

Publications

What's included?

100 Test
Components
Ferric Chloride (2%) Differentiating Solution
1 x 125 mL
Ferric Chloride Solution (10%)
1 x 125 mL
Hematoxylin solution (5%)
1 x 250 mL
Lugol's Iodine Solution
1 x 125 mL
Sodium Thiosulfate Solution (5%)
1 x 125 mL
Van Gieson's Solution
1 x 125 mL

Recommended products

Elastic Stain Kit ab150667 uses a modified Verhoeff's Van Gieson / EVG stain to stain elastic fibers / elastin in tissue sections in histology.

- Use for connective tissue fiber analysis
- Suitable for Formalin-Fixed Paraffin-Embedded (FFPE) sections
- Includes ready-to-use solutions of Cresyl Echt Violet, Luxol Fast Blue, Lithium carbonate and 70% alcohol
- Cited in over 40 publications

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient

Notes

Elastic Stain Kit ab150667 uses a modified Verhoeff's Van Gieson / EVG stain to stain elastic fibers / elastin in tissue sections in histology. Staining of elastic tissue is useful in cases of emphysema (atrophy of elastic tissue), arteriosclerosis (thinning and loss of elastic fibers) and various other vascular diseases.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation
Elastic fibers Black to Blue/Black
NucleiBlue to Black
CollagenRed
Muscle & Other Yellow

ControlTissue: Lung or any vascular tissue.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Elastin also known as tropoelastin is an important protein in connective tissues contributing to the elasticity and flexibility of tissues like skin lungs and arteries. Elastin has a molecular weight of approximately 70 kDa. This protein is primarily expressed in organs that require elastic recoil for normal function. The highest concentrations of elastin can be found in elastic fibers which are critical components of the extracellular matrix of tissues like blood vessels lungs and skin. Elastin fibers can be visualized using specific staining techniques in histological studies such as elastin histology or elastic tissue stains.

Biological function summary

Elastin provides structural integrity and elasticity to connective tissues. It functions as a major component of elastic fibers which are complex structures formed in association with other proteins including fibrillin and microfibril-associated glycoproteins. These components work together to form a supportive mesh in the extracellular matrix. Elastin’s unique properties allow tissues to extend and return to their original shape maintaining tissue flexibility and resilience. Elastic fibers observed through various elastin staining methods help notice elastin’s distribution and organization within tissues.

Pathways

Elastin involves mainly in the extracellular matrix organization and the vascular smooth muscle contraction pathways. Elastin interacts with several proteins such as fibrillin-1 forming complex networks essential for maintaining vascular integrity and elasticity. Matrix metalloproteinases for instance play roles in the remodeling of the extracellular matrix involving elastin degradation and renewal processes. The regulation and interaction of these pathways are key to understanding how elastin supports normal tissue architecture and function.

Associated diseases and disorders

Elastin associates with conditions like cutis laxa and Williams syndrome. Mutations in the elastin gene can lead to cutis laxa a disorder that affects the connective tissue causing loose saggy skin. Williams syndrome is linked to elastin gene deletions and affects cardiovascular connective tissue and neurodevelopmental aspects. These disorders highlight elastin's importance in maintaining healthy tissue structure and function. Associated proteins like fibrillin and lysyl oxidase contribute to understanding these conditions further linking elastin with broader connective tissue abnormalities.

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Histology - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (ab150667), expandable thumbnail

    Histology - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (ab150667)

    ab150667 (Elastic (connective tissue stain)) staining elastic fibres (blue/black) and collagen (red/pink) in formalin fixed paraffin embedded normal human lung. Muscle and other tissues appear yellow.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (ab150667), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (ab150667)

    ab150667 Elastic Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human artery.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (ab150667), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Elastic Stain Kit (Verhoeff Van Gieson / EVG Stain) (ab150667)

    Staining of Uterus with ab150667 - Elastic Stain Kit (Modified Verhoff's).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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