AB117994

ENO1 Assay Kit (alpha Enolase, Human)

Name: ENO1 Assay Kit (alpha Enolase, Human) (ab117994) | Abcam
Brand: Abcam Limited
SKU: AB117994

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(8 Publications)

ENO1 Assay Kit (alpha Enolase, Human) ab117994 is used to determine enolase 1 (ENO1) activity in a sample.

View Alternative Names

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE

4 Images

Functional Studies - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

FuncS

Supplier Data

Functional Studies - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Example of dilution series prepared from HepG2 cells extract.

FuncS

Supplier Data

Functional Studies - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Scheme of reactions used in this kit. Abbreviations are as follows : enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).

FuncS

Supplier Data

Functional Studies - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL. The relative ENO activity was determined from HepG2 standard curve as described in the protocol. The data show that the activity of the neuron-specific ENO2 can’t be detected.

Immunocytochemistry/ Immunofluorescence - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Enzyme activity

Sensitivity

= 125 ng/mL

Range

125 - 2000 ng/mL

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ENO1 Assay Kit (alpha Enolase, Human) ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 63" }, { "sample": "Serum", "range": null, "average": "= 140" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab117994-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Reagent Mix", "number":"AB117994-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"10X Blocking Buffer", "number":"AB117994-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Buffer", "number":"AB117994-CMP02", "productcode":"" }, { "size":"1 x 187.5 Unit", "name":"LDH (Lactate Dehydrogenase)", "number":"AB117994-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Extraction Buffer (ab260490)", "number":"AB117994-CMP03", "productcode":"" }, { "size":"1 x 24 mL", "name":"Base Buffer", "number":"AB117994-CMP06", "productcode":"" }, { "size":"1 x 187.5 Unit", "name":"PK (Pyruvate Kinase)", "number":"AB117994-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate 96 antibody coated wells in 12 strips", "number":"AB117994-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Enolase 1 or alpha-enolase is an important enzyme in the glycolytic pathway. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. We also refer to it as an enolase reaction. ENO1 protein has a molecular mass of approximately 47 kDa and is expressed in most tissues but found most abundantly in the brain muscle and liver. Additionally ENO1 can exist in both cytosolic and plasma membrane-associated forms playing functional roles in different cellular contexts.

Biological function summary

The ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.

Pathways

ENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.

ENO1 is associated with cancer and neurodegenerative diseases. Overexpression or aberrant regulation of ENO1 often correlates with tumor progression and metastasis in cancers such as lung and breast cancer. In neurodegenerative disorders like Alzheimer's disease abnormal functioning of ENO1 can impact brain energy metabolism and neuron degeneration. ENO1's interaction with other proteins such as beta-enolase further connects it to these conditions influencing disease mechanisms and patient outcomes.

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Product protocols

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Target data

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 1369209, PubMed : 29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

See full target information ENO1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110911 PubMed39351192

2024

Sirtuin 1/sirtuin 3 are robust lysine delactylases and sirtuin 1-mediated delactylation regulates glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Runhua Du,Yanmei Gao,Cong Yan,Xuelian Ren,Shankang Qi,Guobin Liu,Xinlong Guo,Xiaohan Song,Hanmin Wang,Jingxin Rao,Yi Zang,Mingyue Zheng,Jia Li,He Huang

Cell & bioscience 14:74 PubMed38849850

2024

Tumour-specific phosphorylation of serine 419 drives alpha-enolase (ENO1) nuclear export in triple negative breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Morgan L Marshall,Kim Yc Fung,David A Jans,Kylie M Wagstaff

EMBO reports 22:e50967 PubMed33372411

2020

Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Yang,Ying Yuan,Hongfeng Yuan,Jiapei Wang,Haolin Yun,Yu Geng,Man Zhao,Linhan Li,Yejing Weng,Zixian Liu,Jinyan Feng,Yanan Bu,Lei Liu,Bingnan Wang,Xiaodong Zhang

Cancers 12: PubMed32013122

2020

Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyi Zhang,Jie Gao,Chuanjing Cheng,Man Zhang,Wenjuan Liu,Xiaoyao Ma,Wei Lei,Erwei Hao,Xiaotao Hou,Yuanyuan Hou,Gang Bai

The Journal of investigative dermatology 139:818-826 PubMed30393078

2018

AHR Regulates Metabolic Reprogramming to Promote SIRT1-Dependent Keratinocyte Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Carrie Hayes Sutter,Kristin M Olesen,Jyoti Bhuju,Zibiao Guo,Thomas R Sutter

Molecular cell 70:663-678.e6 PubMed29775581

2018

p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

He Huang,Shuang Tang,Ming Ji,Zhanyun Tang,Miho Shimada,Xiaojing Liu,Shankang Qi,Jason W Locasale,Robert G Roeder,Yingming Zhao,Xiaoling Li

Frontiers in cellular and infection microbiology 6:153 PubMed27900285

2016

A T4SS Effector Targets Host Cell Alpha-Enolase Contributing to Intracellular Lifestyle.

Applications

Unspecified application

Species

Unspecified reactive species

María I Marchesini,Susana M Morrone Seijo,Francisco F Guaimas,Diego J Comerci

PloS one 8:e66045 PubMed23823123

2013

Systematic Analysis Reveals Elongation Factor 2 and α-Enolase as Novel Interaction Partners of AKT2.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Bottermann,Michael Reinartz,Marian Barsoum,Sebastian Kötter,Axel Gödecke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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ENO1 Assay Kit (alpha Enolase, Human)

Functional Studies - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Functional Studies - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Functional Studies - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Immunocytochemistry/ Immunofluorescence - ENO1 Assay Kit (alpha Enolase, Human) (AB117994)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise