In the Enolase Assay Kit (ab241024) enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product.
View Alternative Names
ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE
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Functional Studies - Enolase Assay Kit (AB241024)
Enolase Activity in rat muscle lysate (1 μg), HeLa lysate (5 μg) and positive control. Assays were performed following the kit protocol.
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Functional Studies - Enolase Assay Kit (AB241024)
H2O2 Standard Curve for Fluorometric Assay.
H2O2 Standard Curve for Fluorometric Assay.
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Functional Studies - Enolase Assay Kit (AB241024)
H2O2 Standard Curve for Colorimetric Assay.
H2O2 Standard Curve for Colorimetric Assay.
Reactivity data
Product details
In the Enolase Assay Kit (ab241024) enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with the probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm).
This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K691 Enolase Activity Colorimetric/Fluorometric Assay Kit. K691-100 is the same size as the 100 test size of ab241024.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Supplementary information
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Biological function summary
The ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.
Pathways
ENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.
Product protocols
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Target data
Publications (4)
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Molecular neurobiology 62:10006-10019 PubMed40180688
2025
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Biomedicines 11: PubMed37189694
2023
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Evidence-based complementary and alternative medicine : eCAM 2022:9302403 PubMed35502178
2022
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Cancer cell 37:599-617.e7 PubMed32243837
2020
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