Enterokinase Activity Assay Kit (Colorimetric)

Product details

Enterokinase Activity Assay Kit (Colorimetric) (ab324118) offers a sensitive assay for quantifying enterokinase activity.

Enterokinase assay principle
The enterokinase assay uses an enzyme substrate that can be cleaved by enterokinase, which specifically recognizes and cleaves at the Asp-Asp-Asp-Asp-Lys site. After cleavage by enterokinase, the enterokinase substrate can be detected by with the addition of EK Yellow in an absorbance microplate reader at 405 nm.

Enterokinase assay protocol summary
- add samples and enterokinase positive control to wells
- add working solution and incubate for 30-60 minutes at 37°C
- analyze with plate reader

Other Notes
Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system. Enterokinase converts trypsinogen into its active form trypsin, resulting in the subsequent activation of pancreatic digestive enzymes. The deficiency of enterokinase results in intestinal digestion impairment. The inhibition of enterokinase may have anti-tumor effects through suppressing proteases involved in carcinogenesis and metastasis.