ER Staining Kit - Red Fluorescence | Cytopainter (ab139482) contains an endoplasmic reticulum (ER)-selective red dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining in immunocytochemistry (ICC).
- Specifically designed for use with GFP-expressing cell lines (including BFPs, CFPs, YFPs)
- Suitable for co-labelling with other fluorescent dyes
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Publications
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- Human molecular genetics 32:3029-30392023Extension of the DNAJB2a isoform in a dominant neuromyopathy family.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaakko Sarparanta,Per Harald Jonson,Jens Reimann,Anna Vihola,Helena Luque,Sini Penttilä,Mridul Johari,Marco Savarese,Peter Hackman,Cornelia Kornblum,Bjarne UddPubMed 37070754
- PLoS biology 20:e30015162022Macrophages take up VLDL-sized emulsion particles through caveolae-mediated endocytosis and excrete part of the internalized triglycerides as fatty acids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Deng,Frank Vrieling,Rinke Stienstra,Guido J Hooiveld,Anouk L Feitsma,Sander KerstenPubMed 36026438
- The Journal of biological chemistry 298:1017272022Structural and functional analysis of the roles of influenza C virus membrane proteins in assembly and budding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Zhang,Tim Abel,Shuo Su,Andreas Herrmann,Kai Ludwig,Michael VeitPubMed 35157850
- Science advances 7:2021Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Feng,Jin Wang,Wei Yang,Jingyu Li,Xiaochen Lin,Fangzi Zha,Xiaolu Wang,Luyao Ma,Nga Ting Choi,Yusuke Mii,Shinji Takada,Michael S Y Huen,Yusong Guo,Liang Zhang,Bo GaoPubMed 33990333
- Cell death & disease 12:4072021FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaeyeon Jang,Yukyung Jun,Suyeon Kim,Eunjeong Kim,Yeonjoo Jung,Byung Jo Park,Jinseon Lee,Jhingook Kim,Sanghyuk Lee,Jaesang KimPubMed 33859174
- Scientific reports 10:72092020N-glycosylation of α-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric M Janezic,Sophia My-Linh Lauer,Robert George Williams,Michael Chungyoun,Kyung-Soon Lee,Edelmar Navaluna,Ho-Tak Lau,Shao-En Ong,Chris HaguePubMed 32350295
- Nature communications 11:20322020Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Bilska et. al.PubMed 32341344
- Scientific reports 10:65772020Hemocyte phagosomal proteome is dynamically shaped by cytoskeleton remodeling and interorganellar communication with endoplasmic reticulum during phagocytosis in a marine invertebrate, Crassostrea gigas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Mao et. al.PubMed 32313134
- BMC molecular and cell biology 21:222020Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEssak S Khan et. al.PubMed 32228452
- Biomolecules 10:2020Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessia Garufi et. al.PubMed 32138264
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
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ER Staining Kit - Red Fluorescence | Cytopainter (ab139482) contains an endoplasmic reticulum (ER)-selective red dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining in immunocytochemistry (ICC).
- Specifically designed for use with GFP-expressing cell lines (including BFPs, CFPs, YFPs)
- Suitable for co-labelling with other fluorescent dyes
Key facts
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based
- Reactive species
- Mammals
- Assay Platform
- Fluorescence microscope
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- -80°C
Notes
ER Staining Kit - Red Fluorescence | Cytopainter (ab139482) contains an endoplasmic reticulum-selective red dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. Micromolar concentrations of the red dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat and human bone osteosarcoma epithelial cell line, U2OS. (Ex/Em = 580/677 nm).
One important application of the ER staining kit is in fluorescence co-localization imaging with green fluorescent protein (GFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
The ER staining kit is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as fluorescein, or coumarin.
A nuclear counterstain is also included in the kit.
Review other dyes and kits for ER staining, or the live cell staining fluorescent dyes guide
Explore our range of organelle-specific antibodies and dyes and discover why our reagents are deeply trusted by cell biologists.
This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the Texas Red region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.
Previously called CytoPainter ER Staining Kit -Red Fluorescence.
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2 product images
Fluorescence Microscopy - ER Staining Kit - Red Fluorescence - Cytopainter (ab139482)
Fluorescent staining using CytoPainter ER Staining kit. Live HeLa cells stained with Red Detection Reagent (A), Hoechst dye (B) and resulting composite image (C).
Fluorescence Microscopy - ER Staining Kit - Red Fluorescence - Cytopainter (ab139482)
Fluorescence excitation and emission spectra for CytoPainter ER Staining kit. Fluorescence excitation and emission spectra for Red dye (panel A) and absorbance and fluorescent emission spectra for Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Buffer.
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