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AB65343

Ethanol Assay Kit

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(50 Publications)

Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.

- Cited in over 40 publications
6 Images
Functional Studies - Ethanol Assay Kit (AB65343)
  • FuncS

Lab

Functional Studies - Ethanol Assay Kit (AB65343)

0.5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured fluorimetrically (background subtracted, duplicates; +/- SD).

Functional Studies - Ethanol Assay Kit (AB65343)
  • FuncS

Supplier Data

Functional Studies - Ethanol Assay Kit (AB65343)

Ethanol Standard Curve (Background subtracted). Expected background (0 nmole EtOH standard) for the standard curve can be around ~0.6-0.7 OD, with a typical dynamic range of 0.7-1.2 after background subtraction. Ethanol standard was diluted as described and the assay was performed according to the kit instructions.

Functional Studies - Ethanol Assay Kit (AB65343)
  • FuncS

Lab

Functional Studies - Ethanol Assay Kit (AB65343)

Standard curves with background signal subtracted (duplicates; +/- SD).

Functional Studies - Ethanol Assay Kit (AB65343)
  • FuncS

PubMed

Functional Studies - Ethanol Assay Kit (AB65343)

Ethanol in blood plasma was analyzed using ethanol assay kit (ab65343). Ethanol concentrations were determined 60 minutes after urethane administration in P0/1 mice and rats.

Zehendner C.M et al., PLoS One 8(5), Fig 5. Doi: 10.1371/journal.pone.0062628 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Ethanol Assay Kit (AB65343)
  • FuncS

Lab

Functional Studies - Ethanol Assay Kit (AB65343)

5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured colourimetrically (background subtracted, duplicates; +/- SD).

Schematic Diagram - Ethanol Assay Kit (AB65343)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Ethanol Assay Kit (AB65343)

Representative image of Ethanol Assay Kit ab65343

Components shown from left to right :

- OxiRed Probe

- Ethanol Standard

- Ethanol Enzyme Mix

- Wash Buffer I

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Food samples, Drinks

Assay type

Quantitative

Sensitivity

> 10 nM

Range

2 - 200 µM

Assay time

1h

Assay Platform

Microplate reader

Product details

Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.

In the ethanol assay protocl, alcohol oxidase oxidizes ethanol to generate H2O2 which reacts with a probe to generate color (absorbance max 570 nm) and fluorescence (Ex/Em 535/587 nm).

The kit detects 0.1-10 ppm alcohol (~10-800 nM).

Ethanol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K620 Ethanol Colorimetric/Fluorometric Assay Kit. K620-100 is the same size as the 100 test size of ab65343.

PLEASE NOTE: Extreme care should be taken to ensure that no alcohol vapors (ethanol, methanol, propanol) are in the laboratory air where this assay is to be performed. Alcohol vapors in the air will be rapidly absorbed by kit components resulting in very high background making the kit unusable. Laboratories where HPLC equipment and solvents are standing or where alcohol is used to wipe down laboratory benches or equipment are inappropriate locations to perform this assay.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "2000Test": { "sellingSize": "2000 Test", "publicAssetCode":"ab65343-2000Test", "assetComponentDetails": [ { "size":"20 x 1 Vial", "name":"Ethanol Enzyme Mix", "number":"AB65343-CMP02" }, { "size":"20 x 25 mL", "name":"Wash Buffer I", "number":"AB65343-CMP01" }, { "size":"20 x 0.5 mL", "name":"Ethanol Standard", "number":"AB65343-CMP04" }, { "size":"20 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65343-CMP03" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65343-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Ethanol Enzyme Mix", "number":"AB65343-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Wash Buffer I", "number":"AB65343-CMP01", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"Ethanol Standard", "number":"AB65343-CMP04", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65343-CMP03", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ethanol also known as ethyl alcohol is a small volatile molecule with a molecular weight of 46.07 g/mol. Ethanol naturally occurs in the fermentation process of yeast and is present in alcoholic beverages. In cellular environments it acts ubiquitously affecting lipid membranes and interacting with various protein structures. Ethanol is not a protein but a small organic compound that exerts mechanical effects on cell membranes and proteins due to its lipophilic nature.
Biological function summary

Ethanol plays a significant role in central nervous system modulation and energy metabolism. It is not a part of a complex but affects neurotransmitter systems by interacting with ion channels and receptors such as GABA and NMDA receptors. The presence of ethanol in biological systems can disrupt normal neural signaling leading to altered mood and cognitive function which is often observed in alcohol consumption.

Pathways

Ethanol impacts several critical biological processes including the central nervous system and metabolic pathways. It influences the gamma-aminobutyric acid (GABA) pathway by enhancing inhibitory neurotransmission leading to sedative effects. Ethanol also participates in the metabolic pathways related to the conversion of alcohol to acetaldehyde and then to acetic acid involving enzymes such as alcohol dehydrogenase and aldehyde dehydrogenase. This metabolic process connects ethanol to energy production and lipid metabolism.

Ethanol consumption is closely linked to alcohol use disorders and liver disease. Chronic exposure to ethanol can lead to liver cirrhosis a condition marked by the progressive destruction of liver tissue. The interaction of ethanol with proteins like cytochrome P450 enzymes can exacerbate liver damage. Ethanol's role in altering GABA receptor activity is also associated with addiction and dependency as it enhances the inhibitory effects leading to the development of tolerance and withdrawal symptoms.

Product protocols

Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 19:1600725 PubMed40535611

2025

Influence of chronic alcohol consumption on cerebral ischemia/reperfusion injury in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Utsab Subedi,Pushpa Subedi,Asia Rogers,Xiao-Hong Lu,Manikandan Panchatcharam,Hong Sun

Nutrients 16: PubMed39408229

2024

Effects of GSH on Alcohol Metabolism and Hangover Improvement in Humans: A Randomized Double-Blind Placebo-Controlled Crossover Clinical Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Gunju Song,Hyein Han,Seyoung Park,Soonok Sa,Wookyung Chung,Boo Yong Lee

Frontiers in behavioral neuroscience 18:1418544 PubMed39206388

2024

Individual corticosterone response to intermittent swim stress predicts a shift in economic demand for ethanol from pre-stress to post-stress in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher L Robison,Victoria Madore,Nicole Cova,Robert C Drugan,Sergios Charntikov

Pharmaceuticals (Basel, Switzerland) 17: PubMed39204192

2024

A Compound Containing Aldehyde Dehydrogenase Relieves the Effects of Alcohol Consumption and Hangover Symptoms in Healthy Men: An Open-Labeled Comparative Study.

Applications

Unspecified application

Species

Unspecified reactive species

In-Kyung Jeong,Anna Han,Ji Eun Jun,You-Cheol Hwang,Kyu Jeung Ahn,Ho Yeon Chung,Bo Seung Kang,Se-Young Choung

Cells 13: PubMed38891060

2024

Mitochondrial Aldehyde Dehydrogenase 2 (ALDH2) Protects against Binge Alcohol-Mediated Gut and Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bipul Ray,Wiramon Rungratanawanich,Karli R LeFort,Saravana Babu Chidambaram,Byoung-Joon Song

International journal of molecular sciences 25: PubMed38542275

2024

TBI and Tau Loss of Function Both Affect Naïve Ethanol Sensitivity in .

Applications

Unspecified application

Species

Unspecified reactive species

Valbona Hoxha,Gaurav Shrestha,Nayab Baloch,Sara Collevechio,Raegan Laszczyk,Gregg Roman

Frontiers in behavioral neuroscience 17:1291128 PubMed38098500

2023

Assessment of ethanol and nicotine interactions using a reinforcer demand modeling with grouped and individual levels of analyses in a long-access self-administration model using male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher L Robison,Nicole Cova,Victoria Madore,Tyler Allen,Scott Barrett,Sergios Charntikov

Nature communications 14:5971 PubMed37749090

2023

A common East-Asian ALDH2 mutation causes metabolic disorders and the therapeutic effect of ALDH2 activators.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Cheng Chang,Hsiao-Lin Lee,Wenjin Yang,Meng-Lun Hsieh,Cai-Cin Liu,Tung-Yuan Lee,Jing-Yong Huang,Jiun-Yi Nong,Fu-An Li,Hsiao-Li Chuang,Zhi-Zhong Ding,Wei-Lun Su,Li-Yun Chueh,Yi-Ting Tsai,Che-Hong Chen,Daria Mochly-Rosen,Lee-Ming Chuang

Cellular and molecular gastroenterology and hepatology 16:685-709 PubMed37460041

2023

Inhibition of Abelson Tyrosine-Protein Kinase 2 Suppresses the Development of Alcohol-Associated Liver Disease by Decreasing PPARgamma Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Malnassy,Claudia R Keating,Shaimaa Gad,Bryan Bridgeman,Aldeb Perera,Wei Hou,Scott J Cotler,Xianzhong Ding,Mashkoor Choudhry,Zhaoli Sun,Anthony J Koleske,Wei Qiu

Alcohol (Fayetteville, N.Y.) 110:65-81 PubMed36898643

2023

Dietary soy prevents fetal demise, intrauterine growth restriction, craniofacial dysmorphic features, and impairments in placentation linked to gestational alcohol exposure: Pivotal role of insulin and insulin-like growth factor signaling networks.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qi,Fusun Gundogan,Jeffrey Gilligan,Suzanne de la Monte
View all publications
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