- Cited in over 40 publications
- FuncS
Lab
Functional Studies - Ethanol Assay Kit (AB65343)
0.5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured fluorimetrically (background subtracted, duplicates; +/- SD).
- FuncS
Supplier Data
Functional Studies - Ethanol Assay Kit (AB65343)
Ethanol Standard Curve (Background subtracted). Expected background (0 nmole EtOH standard) for the standard curve can be around ~0.6-0.7 OD, with a typical dynamic range of 0.7-1.2 after background subtraction. Ethanol standard was diluted as described and the assay was performed according to the kit instructions.
- FuncS
Lab
Functional Studies - Ethanol Assay Kit (AB65343)
Standard curves with background signal subtracted (duplicates; +/- SD).
- FuncS
PubMed
Functional Studies - Ethanol Assay Kit (AB65343)
Ethanol in blood plasma was analyzed using ethanol assay kit (ab65343). Ethanol concentrations were determined 60 minutes after urethane administration in P0/1 mice and rats.
Zehendner C.M et al., PLoS One 8(5), Fig 5. Doi: 10.1371/journal.pone.0062628 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
- FuncS
Lab
Functional Studies - Ethanol Assay Kit (AB65343)
5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured colourimetrically (background subtracted, duplicates; +/- SD).
- Schematic Diagram
Supplier Data
Schematic Diagram - Ethanol Assay Kit (AB65343)
Representative image of Ethanol Assay Kit ab65343
Components shown from left to right :
- OxiRed Probe
- Ethanol Standard
- Ethanol Enzyme Mix
- Wash Buffer I
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.
In the ethanol assay protocl, alcohol oxidase oxidizes ethanol to generate H2O2 which reacts with a probe to generate color (absorbance max 570 nm) and fluorescence (Ex/Em 535/587 nm).
The kit detects 0.1-10 ppm alcohol (~10-800 nM).
Ethanol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K620 Ethanol Colorimetric/Fluorometric Assay Kit. K620-100 is the same size as the 100 test size of ab65343.
PLEASE NOTE: Extreme care should be taken to ensure that no alcohol vapors (ethanol, methanol, propanol) are in the laboratory air where this assay is to be performed. Alcohol vapors in the air will be rapidly absorbed by kit components resulting in very high background making the kit unusable. Laboratories where HPLC equipment and solvents are standing or where alcohol is used to wipe down laboratory benches or equipment are inappropriate locations to perform this assay.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Ethanol plays a significant role in central nervous system modulation and energy metabolism. It is not a part of a complex but affects neurotransmitter systems by interacting with ion channels and receptors such as GABA and NMDA receptors. The presence of ethanol in biological systems can disrupt normal neural signaling leading to altered mood and cognitive function which is often observed in alcohol consumption.
Pathways
Ethanol impacts several critical biological processes including the central nervous system and metabolic pathways. It influences the gamma-aminobutyric acid (GABA) pathway by enhancing inhibitory neurotransmission leading to sedative effects. Ethanol also participates in the metabolic pathways related to the conversion of alcohol to acetaldehyde and then to acetic acid involving enzymes such as alcohol dehydrogenase and aldehyde dehydrogenase. This metabolic process connects ethanol to energy production and lipid metabolism.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (50)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in cellular neuroscience 19:1600725 PubMed40535611
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nutrients 16: PubMed39408229
2024
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in behavioral neuroscience 18:1418544 PubMed39206388
2024
Applications
Unspecified application
Species
Unspecified reactive species
Pharmaceuticals (Basel, Switzerland) 17: PubMed39204192
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cells 13: PubMed38891060
2024
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 25: PubMed38542275
2024
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in behavioral neuroscience 17:1291128 PubMed38098500
2023
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 14:5971 PubMed37749090
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cellular and molecular gastroenterology and hepatology 16:685-709 PubMed37460041
2023
Applications
Unspecified application
Species
Unspecified reactive species
Alcohol (Fayetteville, N.Y.) 110:65-81 PubMed36898643
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com