Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.
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- Frontiers in behavioral neuroscience 17:12911282023Assessment of ethanol and nicotine interactions using a reinforcer demand modeling with grouped and individual levels of analyses in a long-access self-administration model using male rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher L Robison,Nicole Cova,Victoria Madore,Tyler Allen,Scott Barrett,Sergios CharntikovPubMed 38098500
- Nature communications 14:59712023A common East-Asian ALDH2 mutation causes metabolic disorders and the therapeutic effect of ALDH2 activators.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Cheng Chang,Hsiao-Lin Lee,Wenjin Yang,Meng-Lun Hsieh,Cai-Cin Liu,Tung-Yuan Lee,Jing-Yong Huang,Jiun-Yi Nong,Fu-An Li,Hsiao-Li Chuang,Zhi-Zhong Ding,Wei-Lun Su,Li-Yun Chueh,Yi-Ting Tsai,Che-Hong Chen,Daria Mochly-Rosen,Lee-Ming ChuangPubMed 37749090
- Cell host & microbe 31:389-404.e72023Candida albicans-specific Th17 cell-mediated response contributes to alcohol-associated liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuling Zeng,Elisa Rosati,Carina Saggau,Berith Messner,Huikuan Chu,Yi Duan,Phillipp Hartmann,Yanhan Wang,Shengyun Ma,Wendy Jia Men Huang,Jihyung Lee,Sung Min Lee,Raquel Carvalho-Gontijo,Vivian Zhang,Joseph P Hoffmann,Jay K Kolls,Eyal Raz,David A Brenner,Tatiana Kisseleva,Salomé LeibundGut-Landmann,Petra Bacher,Peter Stärkel,Bernd SchnablPubMed 36893735
- American journal of physiology. Gastrointestinal and liver physiology 323:G375-G3862022Urolithin A attenuates severity of chronic pancreatitis associated with continued alcohol intake by inhibiting PI3K/AKT/mTOR signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiddharth Mehra,Supriya Srinivasan,Samara Singh,Zhiqun Zhou,Vanessa Garrido,Iago De Castro Silva,Tulasigeri M Totiger,Austin R Dosch,Xizi Dai,Rajinder K Dawra,Venkatakrishna Rao Jala,Chanjuan Shi,Jashodeep Datta,Michael VanSaun,Nipun Merchant,Nagaraj NagathihalliPubMed 36098401
- Frontiers in immunology 13:8847192022Ethanol Intoxication Impairs Respiratory Function and Bacterial Clearance and Is Associated With Neutrophil Accumulation in the Lung After Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHolly J Hulsebus,Kevin M Najarro,Rachel H McMahan,Devin M Boe,David J Orlicky,Elizabeth J KovacsPubMed 35603143
- iScience 25:1038292022A positive feed-forward loop between and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi-Yao Hsueh,Qiang Huang,Hongli Gong,Yujie Shen,Ji Sun,Hui-Ching Lau,Duo Zhang,Di Tang,Chunping Wu,Yang Guo,Huiying Huang,Pengyu Cao,Lei Tao,Ming Zhang,Liang ZhouPubMed 35198889
- Nutrients 13:2021Combined Water Extracts from Oxidation-Treated Leaves and Branches of Has Anti-Hangover and Liver Protective Effects in Binge Alcohol Intake of Male Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihyun Je,Miyoung Song,Ji Hyeong Baek,Jae Soon Kang,Hye Jin Chung,Kwonsu Lee,Sang Won Park,Hyun Joon KimPubMed 34959956
- Foods (Basel, Switzerland) 10:2021Ethanol-Induced Hepatotoxicity and Alcohol Metabolism Regulation by GABA-Enriched Fermented Root Extract in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaila Boby,Eon-Bee Lee,Muhammad Aleem Abbas,Na-Hye Park,Sam-Pin Lee,Md Sekendar Ali,Seung-Jin Lee,Seung-Chun ParkPubMed 34681429
- Plant & cell physiology 62:1460-14772021Physiological Role of Aerobic Fermentation Constitutively Expressed in an Aluminum-Tolerant Cell Line of Tobacco (Nicotiana tabacum).Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshiyuki Tsuchiya,Takuji Nakamura,Yohei Izumi,Keiki Okazaki,Takuro Shinano,Yasutaka Kubo,Maki Katsuhara,Takayuki Sasaki,Yoko YamamotoPubMed 34184745
- Frontiers in molecular neuroscience 14:6827752021Moderate Ethanol Pre-treatment Mitigates ICH-Induced Injury via ER Stress Modulation in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter Bor-Chian Lin,Po-Kai Wang,Cheng-Yoong Pang,Wei-Fen Hu,Andy Po-Yi Tsai,Adrian L Oblak,Hock-Kean LiewPubMed 34248500
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Food samples, Drinks
- Assay type
Quantitative
- Range
2 - 200 µM
- Assay time
1h
- Sensitivity
> 10 nM
Target data
What's included?
Recommended products
Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Food samples, Drinks
- Assay type
Quantitative
- Range
2 - 200 µM
- Assay time
1h
- Assay Platform
Microplate reader
- Sensitivity
> 10 nM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.
In the ethanol assay protocl, alcohol oxidase oxidizes ethanol to generate H2O2 which reacts with a probe to generate color (absorbance max 570 nm) and fluorescence (Ex/Em 535/587 nm).
The kit detects 0.1-10 ppm alcohol (~10-800 nM).
Ethanol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K620 Ethanol Colorimetric/Fluorometric Assay Kit. K620-100 is the same size as the 100 test size of ab65343.
PLEASE NOTE: Extreme care should be taken to ensure that no alcohol vapors (ethanol, methanol, propanol) are in the laboratory air where this assay is to be performed. Alcohol vapors in the air will be rapidly absorbed by kit components resulting in very high background making the kit unusable. Laboratories where HPLC equipment and solvents are standing or where alcohol is used to wipe down laboratory benches or equipment are inappropriate locations to perform this assay.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ethanol also known as ethyl alcohol is a small volatile molecule with a molecular weight of 46.07 g/mol. Ethanol naturally occurs in the fermentation process of yeast and is present in alcoholic beverages. In cellular environments it acts ubiquitously affecting lipid membranes and interacting with various protein structures. Ethanol is not a protein but a small organic compound that exerts mechanical effects on cell membranes and proteins due to its lipophilic nature.
- Biological function summary
Ethanol plays a significant role in central nervous system modulation and energy metabolism. It is not a part of a complex but affects neurotransmitter systems by interacting with ion channels and receptors such as GABA and NMDA receptors. The presence of ethanol in biological systems can disrupt normal neural signaling leading to altered mood and cognitive function which is often observed in alcohol consumption.
- Pathways
Ethanol impacts several critical biological processes including the central nervous system and metabolic pathways. It influences the gamma-aminobutyric acid (GABA) pathway by enhancing inhibitory neurotransmission leading to sedative effects. Ethanol also participates in the metabolic pathways related to the conversion of alcohol to acetaldehyde and then to acetic acid involving enzymes such as alcohol dehydrogenase and aldehyde dehydrogenase. This metabolic process connects ethanol to energy production and lipid metabolism.
- Associated diseases and disorders
Ethanol consumption is closely linked to alcohol use disorders and liver disease. Chronic exposure to ethanol can lead to liver cirrhosis a condition marked by the progressive destruction of liver tissue. The interaction of ethanol with proteins like cytochrome P450 enzymes can exacerbate liver damage. Ethanol's role in altering GABA receptor activity is also associated with addiction and dependency as it enhances the inhibitory effects leading to the development of tolerance and withdrawal symptoms.
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5 product images
Zehendner C.M et al., PLoS One 8(5), Fig 5. Doi: 10.1371/journal.pone.0062628 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Ethanol Assay Kit (ab65343)
Ethanol in blood plasma was analyzed using ethanol assay kit (ab65343). Ethanol concentrations were determined 60 minutes after urethane administration in P0/1 mice and rats.
Functional Studies - Ethanol Assay Kit (ab65343)
0.5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured fluorimetrically (background subtracted, duplicates; +/- SD).
Functional Studies - Ethanol Assay Kit (ab65343)
5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured colourimetrically (background subtracted, duplicates; +/- SD).
Functional Studies - Ethanol Assay Kit (ab65343)
Standard curves with background signal subtracted (duplicates; +/- SD).
Functional Studies - Ethanol Assay Kit (ab65343)
Ethanol Standard Curve (Background subtracted). Expected background (0 nmole EtOH standard) for the standard curve can be around ~0.6-0.7 OD, with a typical dynamic range of 0.7-1.2 after background subtraction. Ethanol standard was diluted as described and the assay was performed according to the kit instructions.
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