Extracellular Oxygen Consumption Reagent ab197242 is an oxygen-sensitive fluorescent dye; its fluorescence is quenched by oxygen.
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- Adipocyte 11:562-5712022Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialin Sun,Ping Leng,Xiao Li,Qie Guo,Jun Zhao,Yu Liang,Xiaolei Zhang,Xue Yang,Jing LiPubMed 36053001
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- Redox biology 41:1018852021Selective modulation by PARP-1 of HIF-1α-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Manuel Martí,Angel Garcia-Diaz,Daniel Delgado-Bellido,Francisco O'Valle,Ariannys González-Flores,Onintza Carlevaris,José Manuel Rodríguez-Vargas,Jean Christophe Amé,Françoise Dantzer,George L King,Klaudia Dziedzic,Edurne Berra,E de Álava,A T Amaral,Ester M Hammond,F Javier OliverPubMed 33581682
- Theranostics 9:3293-33072019Ferroptosis Promotes Photodynamic Therapy: Supramolecular Photosensitizer-Inducer Nanodrug for Enhanced Cancer Treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhu et. al.PubMed 31244955
- Proceedings of the National Academy of Sciences of 114:8596-86012017Activin signaling mediates muscle-to-adipose communication in a mitochondria dysfunction-associated obesity model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Song et. al.PubMed 28739899
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue, Suspension cells, Purified mitochondria, Adherent cells
- Assay type
- Cell-based (quantitative)
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Extracellular Oxygen Consumption Reagent ab197242 is an oxygen-sensitive fluorescent dye; its fluorescence is quenched by oxygen.
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue, Suspension cells, Purified mitochondria, Adherent cells
- Assay type
- Cell-based (quantitative)
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Extracellular Oxygen Consumption Reagent ab197242 is an oxygen-sensitive fluorescent dye; its fluorescence is quenched by oxygen. The dye is used in a mix-and-read, 96-well fluorescence plate reader assay for the real-time kinetic analysis of extracellular oxygen consumption rates (OCR). The oxygen consumption rate is a measure of the cellular respiration rate, and of mitochondrial function.
The Extracellular Oxygen Consumption Reagent dye is used with an oil layer which is added on top of the assay medium to limit diffusion of oxygen into the assay medium. As mitochondrial respiration depletes the oxygen within the assay medium, quenching of the fluorescent dye is reduced, and the fluorescence signal increases proportionately.
The dye and a suitable oil are available together in Extracellular Oxygen Consumption Assay Kit Extracellular Oxygen Consumption Assay ab197243.
The extracellular oxygen consumption assay that the dye is used in, is optimized for isolated mitochondria and cell cultures, and can be used with tissues, enzyme preparations, and small organisms.
The dye excites at 360-380 nm (max 380) and emits at 630-680 nm (max 650).
Learn more about the full range of in live cells.Or review the full for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
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2 product images
Cellular Activity - Extracellular Oxygen Consumption Reagent (ab197242)
Typical lifetime profile of Extracellular O2 Consumption Assay for adherent cells, treated with different ETC compounds, including Antimycin A (recommended as a Negative Control). The effect of Glucose Oxidase as a positive Signal Control is illustrated schematically.
Cellular Activity - Extracellular Oxygen Consumption Reagent (ab197242)
Excitation and emission spectra of Extracellular O2 Consumption Reagent. Left panel shows normalized excitation (Ex = 360-400nm; Peak 380nm). Right panel shows emission (Em = 630 - 680nm; Peak 650nm) in oxygenated and deoxygenated conditions.
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