F-actin Staining Kit - Blue Fluorescence - Cytopainter

• Fluorescence Microscopy

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Fluorescence Microscopy - F-actin Staining Kit - Blue Fluorescence - Cytopainter (AB112124)

CytoPainter F-actin Labelling Kit - Blue Fluorescence (ab112124) - CPA cells were fixed with 4.0% formaldehyde in a Costar black 96-well plate.
Left panel : Cells were labelled with CytoPainter F-actin Labelling Kit - Blue for 30 minutes. Right panel : Cells were pre-treated for 10 minutes with phalloidin, prior staining with CytoPainter F-actin Labelling Kit - Blue for 30 minutes.

• Fluorescence Microscopy

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Fluorescence Microscopy - F-actin Staining Kit - Blue Fluorescence - Cytopainter (AB112124)

Image : Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)

Preparation :

• Fix in 3%PFA in PBS for 30 min at RT
• Incubate in 7.5% sucrose-PBS for 3h at RT
• Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
• Embed the EBs in tissue-Tek OCT compound
• Cut frozen sections to 4-20 µm thickness

Primary antibody : Rabbit anti laminin alpha 1, 1 : 400
Secondary antibody : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1 : 100
F-actin was stained with CytoPainter blue (ab112124), 1 : 100
Nuclei were counterstained with SYTO 16