F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments.
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- International journal of molecular sciences 24:2023MELAS-Derived Neurons Functionally Improve by Mitochondrial Transfer from Highly Purified Mesenchymal Stem Cells (REC).Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Liu,Jiahao Yang,Yoshinori Otani,Takahiro Shiga,Akihiro Yamaguchi,Yasuaki Oda,Miho Hattori,Tsukimi Goto,Shuichi Ishibashi,Yuki Kawashima-Sonoyama,Takaya Ishihara,Yumi Matsuzaki,Wado Akamatsu,Masashi Fujitani,Takeshi TaketaniPubMed 38139018
- iScience 26:1059932023The mechanosensitive ion channel Piezo1 modulates the migration and immune response of microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhu,Jinghui Guo,Yong Wu,Ting Lei,Jiejun Zhu,Hui Chen,Shashwati Kala,Kin Fung Wong,Chi Pong Cheung,Xiaohui Huang,Xinyi Zhao,Minyi Yang,Lei SunPubMed 36798430
- iScience 25:1050192022Partial prevention of glucocorticoid-induced osteocyte deterioration in young male mice with osteocrin gene therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCourtney M Mazur,Christian D Castro Andrade,Nicha Tokavanich,Tadatoshi Sato,Michael Bruce,Daniel J Brooks,Mary L Bouxsein,Jialiang S Wang,Marc N WeinPubMed 36105586
- Frontiers in immunology 13:8831782022Evasion of NK cell immune surveillance the vimentin-mediated cytoskeleton remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJei-Ming Peng,Ching-Feng Chiu,Jai-Hong Cheng,Hui-Ying Liu,Yin-Lun Chang,Jia-Wun Luo,Yu-Ting Weng,Hao-Lun LuoPubMed 36032170
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e224242022Nephrin expression in human epidermal keratinocytes and its implication in poor wound closure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Young Kim,Eun Jung Lee,Jimyung Seo,Yangsin Lee,Yuri Ahn,Sujin Park,Yu Jeong Bae,Jinu Lee,Beom Jin Lim,Doyoung Kim,Jin Won Cho,Sang Ho OhPubMed 35747929
- Pathogens (Basel, Switzerland) 11:2022Extracellular Vesicles from Induce IL-8 Response in THP-1 Macrophage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSakaorat Lertjuthaporn,Jinjuta Somkird,Kittima Lekmanee,Anyapat Atipimonpat,Kasama Sukapirom,Hathai Sawasdipokin,Supathra Tiewcharoen,Kovit Pattanapanyasat,Ladawan KhowawisetsutPubMed 35745486
- Journal of cell science 135:2022Fluid shear stress regulates the survival of circulating tumor cells via nuclear expansion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZichen Xu,Keming Li,Ying Xin,Kai Tang,Mo Yang,Guixue Wang,Youhua TanPubMed 35510498
- Oxidative medicine and cellular longevity 2021:93979602021VIP Stabilizes the Cytoskeleton of Schlemm's Canal Endothelia via Reducing Caspase-3 Mediated ZO-1 Endolysosomal Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaotong Lou,Qianxue Mou,Bowen Zhao,Jingqiu Huang,Ke Yao,Zhaoxia Luo,Meng Ye,Yuanyuan Hu,Qiming Duan,Xing Li,Zheng Wen,Zhiqi Chen,Hong Zhang,Yin ZhaoPubMed 34552687
- Scientific reports 10:117642020Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErfan Dashtimoghadam et. al.PubMed 32678204
- Frontiers in immunology 9:9492018Epithelial C5aR1 Signaling Enhances Uropathogenic Adhesion to Human Renal Tubular Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Song et. al.PubMed 29765378
Key facts
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Cell-based (qualitative)
- Reactive species
- Mammals
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Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
What's included?
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F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments.
Key facts
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Cell-based (qualitative)
- Reactive species
- Mammals
- Assay Platform
- Fluorescence microscope
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112125 is designed to stain F-actins in fixed cells with green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins. When used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
ab112125 provides all the essential components with an optimized staining protocol, which is robust requiring minimal hands-on time. It is an excellent tool for preserving the fluorescent images of particular cells. The phalloidin conjugate has spectral properties similar to those of FITC (Ex/Em = 500/520 nm). It can also be used for fluorescence microscope demonstrations using FITC filter (Ex/Em = 490/525 nm).
ab112125 should be stored dessicated.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.
- Biological function summary
The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.
- Pathways
F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.
- Associated diseases and disorders
Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.
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Functional Studies - F-actin Staining Kit - Green Fluorescence - Cytopainter (ab112125)
Left Panel: Cells labeled with 1X Green Fluorescent Phalloidin Conjugate for 30 minutes only.
Right Panel: Cells treated with phalloidin for 10 minutes, then stained with Green Fluorescent Phalloidin Conjugate
for 30 minutes.
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