F-actin Staining Kit - Green Fluorescence - Cytopainter
3
(1 Review)
|
(18 Publications)
F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments.
View Alternative Names
Beta-actin, ACTB
- FuncS
Supplier Data
Functional Studies - F-actin Staining Kit - Green Fluorescence - Cytopainter (AB112125)
Left Panel : Cells labeled with 1X Green Fluorescent Phalloidin Conjugate for 30 minutes only.
Right Panel : Cells treated with phalloidin for 10 minutes, then stained with Green Fluorescent Phalloidin Conjugate for 30 minutes.
Product details
F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112125 is designed to stain F-actins in fixed cells with green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins. When used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
ab112125 provides all the essential components with an optimized staining protocol, which is robust requiring minimal hands-on time. It is an excellent tool for preserving the fluorescent images of particular cells. The phalloidin conjugate has spectral properties similar to those of FITC (Ex/Em = 500/520 nm). It can also be used for fluorescence microscope demonstrations using FITC filter (Ex/Em = 490/525 nm).
ab112125 should be stored dessicated.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.
Pathways
F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (18)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in microbiology 16:1558603 PubMed40177487
2025
Applications
Unspecified application
Species
Unspecified reactive species
Science advances 11:eadq2881 PubMed40009679
2025
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in pharmacology 16:1541869 PubMed39963243
2025
Applications
Unspecified application
Species
Unspecified reactive species
Materials today. Bio 28:101213 PubMed39280110
2024
Applications
Unspecified application
Species
Unspecified reactive species
Toxics 12: PubMed39195658
2024
Applications
Unspecified application
Species
Unspecified reactive species
Research (Washington, D.C.) 7:0331 PubMed38550779
2024
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 24: PubMed38139018
2023
Applications
Unspecified application
Species
Unspecified reactive species
iScience 26:105993 PubMed36798430
2023
Applications
Unspecified application
Species
Unspecified reactive species
iScience 25:105019 PubMed36105586
2022
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in immunology 13:883178 PubMed36032170
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com