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AB112125

F-actin Staining Kit - Green Fluorescence - Cytopainter

3

(1 Review)

|

(18 Publications)

F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments.

View Alternative Names

Beta-actin, ACTB

1 Images
Functional Studies - F-actin Staining Kit - Green Fluorescence - Cytopainter (AB112125)
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Supplier Data

Functional Studies - F-actin Staining Kit - Green Fluorescence - Cytopainter (AB112125)

Left Panel : Cells labeled with 1X Green Fluorescent Phalloidin Conjugate for 30 minutes only.
Right Panel : Cells treated with phalloidin for 10 minutes, then stained with Green Fluorescent Phalloidin Conjugate for 30 minutes.

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Cell-based (qualitative)

Assay Platform

Fluorescence microscope

Product details

F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

ab112125 is designed to stain F-actins in fixed cells with green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins. When used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.

ab112125 provides all the essential components with an optimized staining protocol, which is robust requiring minimal hands-on time. It is an excellent tool for preserving the fluorescent images of particular cells. The phalloidin conjugate has spectral properties similar to those of FITC (Ex/Em = 500/520 nm). It can also be used for fluorescence microscope demonstrations using FITC filter (Ex/Em = 490/525 nm).

ab112125 should be stored dessicated.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.
Biological function summary

The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.

Pathways

F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.

Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.

Product protocols

Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
See full target information ACTB

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 16:1558603 PubMed40177487

2025

promotes osteoclast differentiation through DUSP4-MAPK signaling in osseous echinococcosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haohao Sun,Yaqing Liu,Yiping Huang,Kangjun Xiong,Zhendong Zhang,Weishan Wang,Yi Dai,Jing Li,Qi Li,Sibo Wang,Chenhui Shi

Science advances 11:eadq2881 PubMed40009679

2025

Local soft niches in mechanically heterogeneous primary tumors promote brain metastasis via mechanotransduction-mediated HDAC3 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Tang,Yufan Zheng,Guanshuo Hu,Ying Xin,Keming Li,Cunyu Zhang,Xi Chen,Bai Zhang,Xueyi Li,Bing Hu,Qiong Jia,Yong-Ping Zheng,Mo Yang,Youhua Tan

Frontiers in pharmacology 16:1541869 PubMed39963243

2025

ELABELA promotes the migration and homing of bone marrow mesenchymal stem cells to myocardial injury sites through the ERK1/2/miR-299a-5p/Exo70 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Yu Hou,Hao Wu,Shuang-Mei Li,Xiao-Jing Li,Shu-Jun Yang,Xu-Xiang Chen,Chang-Qing Zhou,Hui-Bao Long,Hai-Dong Wu,Jia-Ying Fu,Ya-Jie Guo,Tong Wang

Materials today. Bio 28:101213 PubMed39280110

2024

Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ oligomers into phagocytic clearable plaques for Alzheimer's disease treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Arjun Sabu,Yu Ching Huang,Ramalingam Sharmila,Chih-Yuan Sun,Min-Ying Shen,Hsin-Cheng Chiu

Toxics 12: PubMed39195658

2024

Actin Dysregulation Mediates Nephrotoxicity of Cassiae Semen Aqueous Extracts.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlan Yang,Sheng Xiao,Ludi Li,An Zhu,Wusheng Xiao,Qi Wang

Research (Washington, D.C.) 7:0331 PubMed38550779

2024

DNA-PKcs Phosphorylates Cofilin2 to Induce Endothelial Dysfunction and Microcirculatory Disorder in Endotoxemic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzhen Du,Pingjun Zhu,Yukun Li,Jiachi Yu,Tian Xia,Xing Chang,Hang Zhu,Ruibing Li,Qingyong He

International journal of molecular sciences 24: PubMed38139018

2023

MELAS-Derived Neurons Functionally Improve by Mitochondrial Transfer from Highly Purified Mesenchymal Stem Cells (REC).

Applications

Unspecified application

Species

Unspecified reactive species

Lu Liu,Jiahao Yang,Yoshinori Otani,Takahiro Shiga,Akihiro Yamaguchi,Yasuaki Oda,Miho Hattori,Tsukimi Goto,Shuichi Ishibashi,Yuki Kawashima-Sonoyama,Takaya Ishihara,Yumi Matsuzaki,Wado Akamatsu,Masashi Fujitani,Takeshi Taketani

iScience 26:105993 PubMed36798430

2023

The mechanosensitive ion channel Piezo1 modulates the migration and immune response of microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhu,Jinghui Guo,Yong Wu,Ting Lei,Jiejun Zhu,Hui Chen,Shashwati Kala,Kin Fung Wong,Chi Pong Cheung,Xiaohui Huang,Xinyi Zhao,Minyi Yang,Lei Sun

iScience 25:105019 PubMed36105586

2022

Partial prevention of glucocorticoid-induced osteocyte deterioration in young male mice with osteocrin gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Courtney M Mazur,Christian D Castro Andrade,Nicha Tokavanich,Tadatoshi Sato,Michael Bruce,Daniel J Brooks,Mary L Bouxsein,Jialiang S Wang,Marc N Wein

Frontiers in immunology 13:883178 PubMed36032170

2022

Evasion of NK cell immune surveillance the vimentin-mediated cytoskeleton remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Jei-Ming Peng,Ching-Feng Chiu,Jai-Hong Cheng,Hui-Ying Liu,Yin-Lun Chang,Jia-Wun Luo,Yu-Ting Weng,Hao-Lun Luo
View all publications
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