F-actin Staining Kit - Red Fluorescence - Cytopainter

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - F-actin Staining Kit - Red Fluorescence - Cytopainter (AB112127)

F-actin staining (red) in chondrocytes in growth plate cartilage. Mouse femur bone was fixed with 4% PFA for 72 hours, decalcified and cut into 8-10 μm sections in frozen. Tissue was then stained for 60 minutes with ab112127 before a final wash in PBS.

This image is courtesy of an anonymous Abreview

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - F-actin Staining Kit - Red Fluorescence - Cytopainter (AB112127)

HeLa cells were fixed with 4% PFA for 10 minutes, rinsed with PBS and stained for 60 minutes with ab112127 before a final wash in PBS prior to mounting slides. Images obtained with an Olympus BX61 microscope using a Texas Red filter (595/613nm) - 50ms exposure.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - F-actin Staining Kit - Red Fluorescence - Cytopainter (AB112127)

Images of CPA cells fixed with formaldehyde and stained with ab112127 in a black 96-well plate Left image : Cells labeled with 1X Red Fluorescent Phalloidin Conjugate for 30 min only. Right image : Cells treated with phalloidin for 10 min, then stained with Red Fluorescent Phalloidin Conjugate for 30 min.