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AB325358

Factor Xa Activity Assay Kit (Fluorometric)

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ab325358 Factor Xa Activity Assay Kit (Fluorimetric) utilizes the ability of Factor Xa to cleave a synthetic peptide substrate and release AMC (fluorophore), which can be easily quantified by fluorescence at Ex/Em = 368/460 nm. The fluorometric signal is directly proportional to the Factor Xa activity in samples. Our assay kit can detect as low as 1 ng, or 35 µU, of FXa activity in biological samples.

View Alternative Names

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

3 Images
Inhibition Assay - Factor Xa Activity Assay Kit (Fluorometric) (AB325358)
  • Inhib

Supplier Data

Inhibition Assay - Factor Xa Activity Assay Kit (Fluorometric) (AB325358)

FXa activity was measured in EDTA-treated human plasma samples in the presence and absence of an FXa inhibitor, GGACK Dihydrochloride.

S = Factor Xa Substrate, I = Inhibitor.

Biological Activity - Factor Xa Activity Assay Kit (Fluorometric) (AB325358)
  • Biological Activity

Supplier Data

Biological Activity - Factor Xa Activity Assay Kit (Fluorometric) (AB325358)

Standard curve of AMC (in pmole/well), measured by fluorescence Ex/Em = 368/460 nm. One µmole of AMC corresponds to the hydrolysis of one µmole of Factor Xa substrate.

Other - Factor Xa Activity Assay Kit (Fluorometric) (AB325358)
  • Other

Supplier Data

Other - Factor Xa Activity Assay Kit (Fluorometric) (AB325358)

Abcam’s Factor Xa Activity Assay Kit utilizes the ability of Factor Xa to cleave a synthetic substrate, releasing a fluorophore (AMC), which can be quantified by fluorescence at Ex/Em = 368/460 nm.

Key facts

Detection method

Fluorescent

Assay type

Quantitative

Sensitivity

> 1 ng

Assay time

40m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Factor Xa (FXa) is the activated form of the coagulation factor X (Stuart-Power factor, thrombokinase, prothrombinase, thromboplastin, E.C.3.4.21.6). Factor X, a serine endopeptidase plays an important role at several stages of the coagulation pathway. It acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor antithrombin to inactivate several coagulation factors including factor Xa. Abcam's Factor Xa activity assay kit utilizes the ability of Factor Xa to cleave a synthetic peptide substrate and release AMC (fluorophore), which can be easily quantified by fluorescence at Ex/Em = 368/460 nm. The fluorometric signal is directly proportional to the Factor Xa activity in samples. Our assay kit can detect as low as 1 ng, or 35 µU, of FXa activity in biological samples.

Heparin-treated plasma is not recommended for the assay. This is because Heparin binds to plasma cofactor antithrombin to inactivate several coagulation factors, including Factor Xa.

•    Thaw kit components and aliquot components susceptible to freeze/thaws; get equipment ready.
•    Prepare standard curve and positive control reactions.
•    Prepare samples in duplicate (find optimal dilutions to fit standard curve readings).
•    Set up plate for standard, positive control, and samples (50 µL).
•    Prepare FXa Reaction Mix.

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What's included?

{ "values": { "1x100Tests": { "sellingSize": "1 x 100 Tests", "publicAssetCode":"ab325358-1x100Tests", "assetComponentDetails": [ { "size":"1 x 5 µL", "name":"FXa Enzyme", "number":"AB325358-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard (1 mM AMC in DMSO)", "number":"AB325358-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"Fxa Substrate", "number":"AB325358-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"FXa Assay Buffer", "number":"AB325358-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"FXa Dilution Buffer", "number":"AB325358-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).
See full target information F10
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