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AB241037

Factor XIa Assay Kit

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The Factor XIa Assay Kit (ab241037) utilizes the ability of factor XIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD 405 nm).

View Alternative Names

Coagulation factor XI, FXI, Plasma thromboplastin antecedent, PTA, F11

4 Images
Functional Studies - Factor XIa Assay Kit (AB241037)
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Functional Studies - Factor XIa Assay Kit (AB241037)

Standard curve for pNA (n = 3) was used to estimate FXIa activity in plasma (n = 5). Assays were performed according to the kit protocol.

Functional Studies - Factor XIa Assay Kit (AB241037)
  • FuncS

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Functional Studies - Factor XIa Assay Kit (AB241037)

Kinetic progressive curves for different amounts of FXIa Enzyme.

Functional Studies - Factor XIa Assay Kit (AB241037)
  • FuncS

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Functional Studies - Factor XIa Assay Kit (AB241037)

Kinetic progressive curves for different amounts Activated Plasma Samples.

Functional Studies - Factor XIa Assay Kit (AB241037)
  • FuncS

Unknown

Functional Studies - Factor XIa Assay Kit (AB241037)

Standard curve for pNA.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Purified protein

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Secondary Antibodies

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Primary Antibodies

AB232726

Anti-Factor XIa antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIa antibody (AB232726)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Factor XIa Assay Kit (ab241037) utilizes the ability of factor XIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD 405 nm).

The kit is easy-to-use and can detect Factor XIa (as low as 1 mPEU) from plasma and purified protein samples.

This product is manufactured by BioVision, an Abcam company and was previously called K973 Factor XIa Activity Assay Kit (Colorimetric). K973-100 is the same size as the 100 test size of ab241037.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241037-100Test", "assetComponentDetails": [ { "size":"1 x 20 µL", "name":"pNA Standard I", "number":"AB241037-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"PK Inhibitor", "number":"AB241037-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Factor XIa", "number":"AB241037-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"FXIIa Inhibitor", "number":"AB241037-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"FXIa Substrate", "number":"AB241037-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"FXIa Assay Buffer", "number":"AB241037-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"FXIa Activator", "number":"AB241037-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XIa also known as Coagulation Factor XIa or FXIa plays an important role in the blood coagulation cascade. This serine protease has a molecular weight of approximately 160 kDa and derives from the zymogen Factor XI. Factor XIa is synthesized in the liver and circulates in the blood plasma. In its active form it cleaves the Arg369-Ile370 bond in Factor IX leading to the further activation of the coagulation pathway.
Biological function summary

Factor XIa is essential for hemostasis. It forms a part of the intrinsic pathway complex that contributes to the amplification of thrombin generation. This activity strengthens the formation of a stable fibrin clot which is critical to prevent excessive bleeding. Factor XIa is also involved in cellular response mechanisms beyond coagulation such as inflammatory processes.

Pathways

Factor XIa is a significant component of the intrinsic coagulation pathway and the contact activation system. It works closely with proteins like Factor IX and kallikrein facilitating the transformation of inactive proteins to their active states in a stepwise manner. These interactions support hemostatic balance and help maintain proper clot formation while preventing thrombosis.

Factor XIa has connections to thrombotic conditions such as deep vein thrombosis and ischemic stroke. Excessive activation of FXIa can contribute to pathological clot formation. Inhibitors like anti-XIa reduce activity and are explored for therapeutic interventions in these conditions. Additionally congenital Factor XI deficiency often called Hemophilia C presents a bleeding disorder that highlights FXIa's significance in coagulation.
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Product protocols

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Target data

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
See full target information F11
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websiteProtocolBooklet
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Product promise

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