Factor XIIa Assay Kit ab241041 uses the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm).
Images
Key facts
- Detection method
Colorimetric
- Sample types
Plasma
- Reactive species
Mammals
Target data
Function
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Alternative names
Coagulation factor XII, Hageman factor, HAF, F12
What's included?
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Factor XIIa Assay Kit ab241041 uses the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm).
Alternative names
Coagulation factor XII, Hageman factor, HAF, F12
Key facts
- Detection method
Colorimetric
- Sample types
Plasma
- Reactive species
Mammals
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
The Factor XIIa Assay Kit (ab241041) utilizes the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma samples. This kit also measures Factor XII. In the sample preparation, the FXII is converted to FXIIa by the FXII activator.
This product is manufactured by BioVision, an Abcam company and was previously called K994 Factor XIIa Activity Assay Kit (Colorimetric). K994-100 is the same size as the 100 test size of ab241041.
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4 product images
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
Kinetic progressive curves for different amounts of FXIIa Enzyme
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
Kinetic progressive curves for different amounts of Activated Plasma Samples are shown.
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
Standard curve for pNA.
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
PK activity. Standard curve for pNA (n = 3) was used to estimate FXIIa activity in Normal Pooled Human Plasma and FXII-deficient Human Plasma (n = 3) (D). Assays were performed according to the kit protocol.
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