Factor XIIa Assay Kit ab241041 uses the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm).
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma
- Reactive species
- Mammals
Target data
Function
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (PubMed:2019570, PubMed:21304106, PubMed:8427954).
Alternative names
Coagulation factor XII, Hageman factor, HAF, F12
What's included?
Recommended products
Factor XIIa Assay Kit ab241041 uses the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm).
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma
- Reactive species
- Mammals
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Factor XIIa Assay Kit (ab241041) utilizes the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma samples. This kit also measures Factor XII. In the sample preparation, the FXII is converted to FXIIa by the FXII activator.
This product is manufactured by BioVision, an Abcam company and was previously called K994 Factor XIIa Activity Assay Kit (Colorimetric). K994-100 is the same size as the 100 test size of ab241041.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
Kinetic progressive curves for different amounts of FXIIa Enzyme
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
Kinetic progressive curves for different amounts of Activated Plasma Samples are shown.
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
Standard curve for pNA.
Functional Studies - Factor XII / XIIa Assay Kit (ab241041)
PK activity. Standard curve for pNA (n = 3) was used to estimate FXIIa activity in Normal Pooled Human Plasma and FXII-deficient Human Plasma (n = 3) (D). Assays were performed according to the kit protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com