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AB252895

Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric)

4

(1 Review)

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(3 Publications)

In Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (ab252895), FAAH hydrolyzes a non-fluorescent substrate releasing 7-amino-4-methylcoumarin (AMC), a fluorophore, which can be easily measured at Ex/Em= 360/465 nm.

View Alternative Names

FAAH1, FAAH, Fatty-acid amide hydrolase 1, Anandamide amidohydrolase 1, Fatty acid ester hydrolase, Oleamide hydrolase 1

3 Images
Functional Studies - Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (AB252895)
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Supplier Data

Functional Studies - Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (AB252895)

Kinetic measurement of FAAH activity.

Lysates prepared from blank (no sample), rat liver microsome (RLM : 32 μg) and rat liver lysate (RLL : 80 μg) and FAAH Positive Control.

Functional Studies - Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (AB252895)
  • FuncS

Supplier Data

Functional Studies - Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (AB252895)

AMC standard curve.

Functional Studies - Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (AB252895)
  • FuncS

Supplier Data

Functional Studies - Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (AB252895)

FAAH specific activity.

Activity of two rat liver microsome preparations (RLM1 and RLM2) and rat liver lysate (RLL : 80 μg).

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Microsomes, Tissue Homogenate, Adherent cells

Sensitivity

= 0.1 µU

Assay Platform

Microplate

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Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)

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Primary Antibodies

AB259323

Anti-Cannabinoid Receptor I antibody [EPR23934-20]

RabMAb|Recombinant

Western blot - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (AB259323)

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Primary Antibodies

AB128917

Anti-FAAH1 antibody [EPR7549]

RabMAb|Recombinant

Western blot - Anti-FAAH1 antibody [EPR7549] (AB128917)

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Assay Kits

AB65393

LDH Assay Kit (Cytotoxicity)

Biochemical assay - LDH Assay Kit (Cytotoxicity) (AB65393)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

In Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric) (ab252895), FAAH hydrolyzes a non-fluorescent substrate releasing 7-amino-4-methylcoumarin (AMC), a fluorophore, which can be easily measured at Ex/Em= 360/465 nm. The kit provides a specific inhibitor that can be used to compensate for potential non-specfic background in unknown samples. The stable fluorescence signal is positively correlated to FAAH enzymatic activity in samples. The kit offers a rapid, simple, sensitive, reproducible assay and is suitable for detecting FAAH activity as low as 0.1 μU.

This product is manufactured by BioVision, an Abcam company and was previously called K434 Fatty Acid Amide Hydrolase (FAAH) Activity Assay Kit (Fluorometric). K434-100 is the same size as the 100 test size of ab252895.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab252895-100Test", "assetComponentDetails": [ { "size":"1 x 40 µL", "name":"FAAH Positive Control", "number":"AB252895-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"FAAH Substrate", "number":"AB252895-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"FAAH Inhibitor", "number":"AB252895-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 59", "number":"AB252895-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard", "number":"AB252895-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fatty acid amide hydrolase 1 (FAAH1) often referred to simply as FAAH is an enzyme that plays a critical role in the degradation of fatty acid amides such as anandamide in the body. This enzyme weighing approximately 63 kDa is a part of the amidase signature family and is characterized by its amidase hydrolase activity. FAAH1 is predominantly expressed in the nervous system liver and gastrointestinal tract where it hydrolyses amide bonds facilitating the termination of signaling pathways initiated by neurotransmitter-like fatty acid amides.
Biological function summary

FAAH1 is essential for maintaining homeostasis by modulating levels of bioactive lipids. It exerts control over endocannabinoid signaling by breaking down anandamide a lipid derivative functioning as a neurotransmitter. FAAH1 does not act as part of a larger protein complex. Instead it operates alone to regulate the duration and intensity of signaling rather than relying on direct interactions within a complex. The enzyme's function impacts the neurochemical milieu in tissues where it is actively expressed thereby affecting mood pain sensation and more.

Pathways

FAAH1 has a significant role in the endocannabinoid signaling pathway and fatty acid metabolism. Within these pathways it modulates signaling by hydrolysing bioactive amides like anandamide influencing overall lipid signaling. FAAH1 activities intersect with several other proteins such as cannabinoid receptors which bind the substrates that FAAH1 eventually degrades. These interactions are vital in controlling signal transduction processes that regulate physiological responses including stress and analgesia.

FAAH1 has been implicated in conditions like chronic pain and anxiety disorders. Altered FAAH1 function or expression can lead to dysregulation of endocannabinoid signaling contributing to the pathophysiology of these conditions. The enzyme's modulation of anandamide levels connects it to proteins such as CB1 and CB2 cannabinoid receptors which mediate the effects of endocannabinoids in neurological and psychiatric disorders. Research into FAAH1 inhibitors as therapeutic agents highlights its importance in potential treatments for managing pain and anxiety.
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Product protocols

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Target data

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed : 17015445, PubMed : 19926788, PubMed : 9122178). Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed : 17015445, PubMed : 9122178). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed : 21049984). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).
See full target information FAAH
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36611887

2022

Kidney Proximal Tubule GLUT2-More than Meets the Eye.

Applications

Unspecified application

Species

Unspecified reactive species

Majdoleen Ahmad,Ifat Abramovich,Bella Agranovich,Alina Nemirovski,Eyal Gottlieb,Liad Hinden,Joseph Tam

Nature communications 13:6941 PubMed36396957

2022

The endocannabinoid anandamide is an airway relaxant in health and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Simon,Thomas von Einem,Alexander Seidinger,Michaela Matthey,Laura Bindila,Daniela Wenzel

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 48:897-907 PubMed36114286

2022

Anandamide and 2-arachidonoylglycerol differentially modulate autistic-like traits in a genetic model of autism based on FMR1 deletion in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Schiavi,Antonia Manduca,Emilia Carbone,Valeria Buzzelli,Alessandro Rava,Alessandro Feo,Fabrizio Ascone,Maria Morena,Patrizia Campolongo,Matthew N Hill,Viviana Trezza
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