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AB118183

Fatty Acid Oxidation Assay Kit (flow cytometry)

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(13 Publications)

Fatty Acid Oxidation Assay Kit ab118183 contains antibodies against key enzymes of the mitochondrial fatty acid oxidation pathway for flow cytometry.

View Alternative Names

MCAD, Medium chain acyl-CoA dehydrogenase, MCADH, ACADM

7 Images
Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)

Expression levels of ACADVL was determined in primary fibroblasts by flow cytometry. Blue : control cell line. Red : deficient cell lines extracted from patients with well characterized mutations in each of the enzymes ACADVL : p[N122D]. Black : Background fluorescence was determined with the supplied negative control.

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)

After background subtraction, the ACADVL deficient cell line shows a 93% reduction in the level of the ACADVL protein.

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)

After background subtraction, the ACADM deficient cell line shows a 57% reduction in the level of the ACADM protein.

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)

Expression levels of ACADM was determined in primary fibroblasts by flow cytometry. Blue : control cell line. Red : deficient cell lines extracted from patients with well characterized mutations in each of the enzymes ACADM : p[K604E] Black : Background fluorescence was determined with the supplied negative control.

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)

After background subtraction, the LCHAD deficient cell line shows a 50% reduction in the level of the HADHA protein.

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)

Expression levels of HADHA was determined in primary fibroblasts by flow cytometry. Blue : control cell line. Red : deficient cell lines extracted from patients with well characterized mutations in each of the enzymes HADHB : p[R61H];[R247H]. Black : Background fluorescence was determined with the supplied negative control.

Immunocytochemistry/ Immunofluorescence - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Fatty Acid Oxidation Assay Kit (flow cytometry) (AB118183)

Antibody specificity demonstrated by immunocytochemistry. Cells were processes with the Flow cytometry protocol as explained above. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Quantitative

Assay Platform

Flow cytometer

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Product details

Fatty Acid Oxidation Assay Kit ab118183 contains antibodies against key enzymes of the mitochondrial fatty acid oxidation pathway for flow cytometry. The assay combines the power of single cell analysis obtained from flow cytometry with the specificity of antibody-based immunostaining to quantify protein levels in cultured cells. Cells are harvested and fixed/permeabilized in suspension, targets of interest are detected indirectly with highly specific well-characterized monoclonal antibodies that are then labeled with fluorescent antibodies

This product was previously called Fatty Acid Oxidation Human Flow Cytometry Kit.

Spin down the contents of the antibody vials upon receipt of the kit. Store all components upright at 4°C. This kit is stable for at least 6 months from receipt.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab118183-96Test", "assetComponentDetails": [ { "size":"1 x 0.12 mL", "name":"100X ACADM Primary Antibody", "number":"AB118183-CMP02", "productcode":"" }, { "size":"1 x 0.12 mL", "name":"100X Normal Mouse IgG", "number":"AB118183-CMP07", "productcode":"" }, { "size":"1 x 0.12 mL", "name":"100X ACADVL Primary Antibody", "number":"AB118183-CMP03", "productcode":"" }, { "size":"1 x 4 mL", "name":"400X Tween-20", "number":"AB118183-CMP04", "productcode":"" }, { "size":"1 x 0.12 mL", "name":"100X HADHA Primary Antibody", "number":"AB118183-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Blocking Solution", "number":"AB118183-CMP06", "productcode":"" }, { "size":"1 x 100 mL", "name":"10X Phosphate Buffered Saline", "number":"AB118183-CMP01", "productcode":"" }, { "size":"1 x 1.25 mL", "name":"100X Triton X-100", "number":"AB118183-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 1970566, PubMed : 21237683, PubMed : 2251268, PubMed : 8823175). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed : 2251268). Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed : 15159392, PubMed : 25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains (PubMed : 1970566, PubMed : 21237683, PubMed : 2251268, PubMed : 8823175).
See full target information ACADM

Additional targets

HADHA,ACADVL
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:175 PubMed39975955

2025

Hypoxia‑induced SREBP1‑mediated lipogenesis and autophagy promote cell survival via fatty acid oxidation in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Ha Jung,Yeseul Yang,Yongbaek Kim

Rheumatology (Oxford, England) 63:2578-2589 PubMed38837706

2024

FTY720 ameliorates experimental MPO-ANCA-associated vasculitis by regulating fatty acid oxidation via the neutrophil PPARα-CPT1a pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Xue Wang,Luo-Yi Wang,Xiang-Yu Han,Su-Fang Chen,Xiao-Jing Sun,Zhi-Ying Li,Mark A Little,Ming-Hui Zhao,Min Chen

Nature communications 14:2980 PubMed37221214

2023

Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong-Su Park,Jun-Hyeong Kim,Won-Chang Soh,Na-Young Kim,Kyung-Sik Lee,Chang-Hyun Kim,Ik-Joo Chung,Sunjae Lee,Hye-Ran Kim,Chang-Duk Jun

Cancer communications (London, England) 42:848-867 PubMed35904817

2022

PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Weixing Dai,Wenqiang Xiang,Lingyu Han,Zixu Yuan,Renjie Wang,Yanlei Ma,Yongzhi Yang,Sanjun Cai,Ye Xu,Shaobo Mo,Qingguo Li,Guoxiang Cai

iScience 24:102766 PubMed34286232

2021

Programmed PPAR-α downregulation induces inflammaging by suppressing fatty acid catabolism in monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Wang,Yan Yan,Zhengguo Zhang,Xiaohan Yao,Xixi Duan,Ziming Jiang,Junfeng An,Peiguo Zheng,Yijie Han,Hao Wu,Zhaoqing Wang,Rainer Glauben,Zhihai Qin

Frontiers in immunology 11:583276 PubMed33363533

2020

Corticosterone Enhances the AMPK-Mediated Immunosuppressive Phenotype of Testicular Macrophages During Uropathogenic Induced Orchitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengguo Zhang,Ziming Jiang,Yiming Zhang,Yu Zhang,Yan Yan,Sudhanshu Bhushan,Andreas Meinhardt,Zhihai Qin,Ming Wang

Journal of Zhejiang University. Science. B 21:885-896 PubMed33150772

2020

Novel ACADVL variants resulting in mitochondrial defects in long-chain acyl-CoA dehydrogenase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Chen,Fan Tong,Xiao-Yu Wu,Ling Zhu,Qiu-Zi Yi,Jing Zheng,Ru-Lai Yang,Zheng-Yan Zhao,Xiao-Hui Cang,Qiang Shu,Ping-Ping Jiang

Scientific reports 9:13188 PubMed31515494

2019

Assessment of temporal functional changes and miRNA profiling of human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Naresh Kumar,Julie A Dougherty,Heather R Manring,Ibrahim Elmadbouh,Muhamad Mergaye,Andras Czirok,Dona Greta Isai,Andriy E Belevych,Lianbo Yu,Paul M L Janssen,Paolo Fadda,Sandor Gyorke,Maegen A Ackermann,Mark G Angelos,Mahmood Khan

Cell communication and signaling : CCS 17:87 PubMed31358011

2019

Connecting lysosomes and mitochondria - a novel role for lipid metabolism in cancer cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Bartel,Helmut Pein,Bastian Popper,Sabine Schmitt,Sudha Janaki-Raman,Almut Schulze,Florian Lengauer,Andreas Koeberle,Oliver Werz,Hans Zischka,Rolf Müller,Angelika M Vollmar,Karin von Schwarzenberg

Carcinogenesis 39:826-837 PubMed29668854

2018

Prostate cancer chemoprevention by sulforaphane in a preclinical mouse model is associated with inhibition of fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Krishna B Singh,Su-Hyeong Kim,Eun-Ryeong Hahm,Subrata K Pore,Bruce L Jacobs,Shivendra V Singh
View all publications
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