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AB287857

Fatty Acid Uptake Assay Kit

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(1 Publication)

Fatty Acid Uptake Assay Kit (ab287857) enables rapid determination of real-time fatty acid uptake kinetics in live cells expressing fatty acid transporter proteins.
3 Images
Functional Studies - Fatty Acid Uptake Assay Kit (AB287857)
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Functional Studies - Fatty Acid Uptake Assay Kit (AB287857)

Endpoint fluorescence in 3T3-L1 adipocytes in the presence of ascending doses of insulin (0, 25 and 250 nM), as well as a mixture of insulin and wortmannin (250 nM + 5 μM). Fluorescence was measured 60 minutes after the addition of Uptake Reaction Mix. Data are mean RFU ± SEM of 4 independent plates, with each data point representing the mean of quadruplicate wells.

Functional Studies - Fatty Acid Uptake Assay Kit (AB287857)
  • FuncS

Unknown

Functional Studies - Fatty Acid Uptake Assay Kit (AB287857)

Kinetics of fatty acid uptake in serum-starved 3T3-L1 fibroblasts and differentiated 3T3-L1 adipocytes treated with insulin (25 and 250 nM) or insulin plus wortmannin (250 nM + 5 μM), a PI3-Kinase inhibitor that blocks insulin signaling pathways.

Fluorescence Microscopy - Fatty Acid Uptake Assay Kit (AB287857)
  • Fluorescence Microscopy

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Fluorescence Microscopy - Fatty Acid Uptake Assay Kit (AB287857)

Fluorescence imaging showing the extent of fluorescent fatty acid analogue uptake after 60 minutes. (i) undifferentiated 3T3-L1 fibroblasts, (ii-iv) differentiated 3T3-L1 adipocytes pretreated with (ii) vehicle (medium w/ 0.1% DMSO), (iii) 250 nM insulin or (iv) 250 nM insulin and 5 μM wortmannin. Fluorescence images were obtained with a Nikon TE2000 inverted microscope using a 20X Plan Fluor objective and a FITC filter cube.

Key facts

Detection method

Fluorescent

Sample types

Adherent cells

Reacts with

Mammals

Assay type

Cell-based

Assay Platform

Microplate (12 x 8 well strips)

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Product details

Fatty Acid Uptake Assay Kit (ab287857) enables rapid determination of real-time fatty acid uptake kinetics in live cells expressing fatty acid transporter proteins. The assay uses a highly fluorescent long-chain fatty acid analogue (Ex/Em = 488/523 nm) that acts as a substrate for transmembrane fatty acid transporters (FATPs) and accumulates in intracellular lipid droplets. A proprietary non-toxic membrane-impermeable quenching agent eliminates any fluorescence arising from the extracellular space, ensuring specific measurement of intracellular fatty acid accumulation without requiring any tedious wash steps. The assay is highly sensitive, has a simple "mix-and-read" protocol and is highthroughput adaptable for screening of fatty acid uptake-modulating test compounds or treatment conditions. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab287857-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"FA Uptake Assay Buffer", "number":"AB287857-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"200X Fluorescent Fatty Acid Probe", "number":"AB287857-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X Extracellular Quenching Solution", "number":"AB287857-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:409 PubMed38992688

2024

Targeting CSF1R in myeloid-derived suppressor cells: insights into its immunomodulatory functions in colorectal cancer and therapeutic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tong,Shifeng Qiao,Zhe Dong,Xiaohui Zhao,Xiaxia Du,Wei Niu
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Product promise

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