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AB325573

Fibronectin Cell Adhesion Assay (Colorimetric)

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ab325573 Fibronectin Cell Adhesion Assay (Colorimetric) kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 Human Fibronectin-coated wells, 8 BSA-coated wells).
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Functional Studies - Fibronectin Cell Adhesion Assay (Colorimetric) (AB325573)
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Functional Studies - Fibronectin Cell Adhesion Assay (Colorimetric) (AB325573)

ECM-mediated Cell Adhesion. Serum starved cells were allowed to attach to ECM-coated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells were stained (left panel picture) and quantified at OD 560nm after extraction (right panel figure).

This figure demonstrates typical results with ab325573 Fibronectin Cell Adhesion Assay (Colorimetric). One should use the data below for reference only. This data should not be used to interpret actual results.

Key facts

Detection method

Colorimetric

Assay type

Cell-based

Results type

Quantitative

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cellular Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival.

ab325573 Fibronectin Cell Adhesion Assay (Colorimetric) kit utilizes a Fibronectin-coated 48-well plate. First, cells are seeded onto the coated substrate, where the adherent cells are captured. Next, unbound cells are washed away, and the adherent cells are fixed/stained. Finally, the stain is extracted and quantified colorimetrically.

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What's included?

{ "values": { "48Tests": { "sellingSize": "48 Tests", "publicAssetCode":"ab325573-48Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Fibronectin Adhesion Plate", "number":"AB325573-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Extraction Solution", "number":"AB325573-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Stain Solution", "number":"AB325573-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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