JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325574

Fibronectin Cell Adhesion Assay (Fluorometric)

Be the first to review this product! Submit a review

|

(0 Publication)

ab325574 Fibronectin Cell Adhesion Assay (Fluorometric) kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 Human Fibronectin-coated wells, 8 BSA-coated wells).
2 Images
Functional Studies - Fibronectin Cell Adhesion Assay (Fluorometric) (AB325574)
  • FuncS

Supplier Data

Functional Studies - Fibronectin Cell Adhesion Assay (Fluorometric) (AB325574)

ECM-mediated Cell Adhesion. Serum starved cells were allowed to attach to ECM-coated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells were lysed and quantified by GR Dye as described in Assay Protocol.

This figure demonstrates typical results with ab325574 Fibronectin Cell Adhesion Assay (Fluorometric). One should use the data below for reference only. This data should not be used to interpret actual results.

Functional Studies - Fibronectin Cell Adhesion Assay (Fluorometric) (AB325574)
  • FuncS

Supplier Data

Functional Studies - Fibronectin Cell Adhesion Assay (Fluorometric) (AB325574)

Quantitation of Human HT-1080. HT-1080 cells were titrated in 1X PBS, then subsequently lysed and detected with 1X Lysis Buffer/GR Dye (225 µL of cell suspension was mixed with 75 µL of 4X Lysis Buffer and 1 µL of GR dye).

This figure demonstrates typical results with ab325574 Fibronectin Cell Adhesion Assay (Fluorometric). One should use the data below for reference only. This data should not be used to interpret actual results.

Key facts

Detection method

Fluorometric

Assay type

Cell-based

Results type

Quantitative

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cellular Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival.

ab325574 Fibronectin Cell Adhesion Assay (Fluorometric) kit utilizes a Fibronectin-coated 48-well plate. First, cells are seeded onto the coated substrate, where the adherent cells are captured. Next, unbound cells are removed with consecutive washes. Finally, the adherent cells are lysed and subsequently detected with GR Dye.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "48Tests": { "sellingSize": "48 Tests", "publicAssetCode":"ab325574-48Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"4X Lysis Solution", "number":"AB325574-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"GR Dye", "number":"AB325574-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibronectin Adhesion Plate", "number":"AB325574-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com