Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry.
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Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation.
Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is designed to label cells at Ex/ Em =353/442 nm excited with a laser at 335 nm.
**Related assays** Review the to learn about kits to perform a , and .
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3 product images
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741)
Spectrum for ab176741.
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741)
Fluorescent analysis of HeLa cells fixed with formaldehyde and labeled with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue) in a Costar black walled/clear bottom 96-well plate.
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741)
Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741). Jurkat cells were treated and stained by Blue Viability Dye. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry as described above. Live (Green) and Dead (heat-treated, Red) cells were distinguished with Pacific Blue channel. The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.
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