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Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry.

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Images

Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (AB176741), expandable thumbnail
  • Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (AB176741), expandable thumbnail
  • Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (AB176741), expandable thumbnail

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based (quantitative)
Reactive species
Mammals, Human

Reactivity data

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

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What's included?

200 Test
Components
DMSO
1 x 200 µL
Tracking dye Blue
1 x 1 Vial

Recommended products

Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry.

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based (quantitative)
Reactive species
Mammals, Human
Assay Platform
Flow cytometer

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation.

Fixable Cell Viability Assay Kit (Fluorometric - Blue) | Cytopainter (ab176741) is designed to label cells at Ex/ Em =353/442 nm excited with a laser at 335 nm.

**Related assays** Review the to learn about kits to perform a , and .

Product promise

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Full details and terms and conditions can be found here:
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3 product images

  • Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741), expandable thumbnail

    Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741)

    Spectrum for ab176741.

  • Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741), expandable thumbnail

    Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741)

    Fluorescent analysis of HeLa cells fixed with formaldehyde and labeled with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue) in a Costar black walled/clear bottom 96-well plate.

  • Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741), expandable thumbnail

    Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741)

    Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741). Jurkat cells were treated and stained by Blue Viability Dye. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry as described above. Live (Green) and Dead (heat-treated, Red) cells were distinguished with Pacific Blue channel. The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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