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AB176741

Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation)

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Abcam's Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry.
3 Images
Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 335 nm) - Cytopainter (ab176741). Jurkat cells were treated and stained by Blue Viability Dye. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry as described above. Live (Green) and Dead (heat-treated, Red) cells were distinguished with Pacific Blue channel. The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.

Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)

Spectrum for ab176741.

Fluorescence Microscopy - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (AB176741)

Fluorescent analysis of HeLa cells fixed with formaldehyde and labeled with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue) in a Costar black walled/clear bottom 96-well plate.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (ab176741) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation.

Fixable Cell Viability Assay Kit ( Blue Fluorescence, for 335 nm excitation) (ab176741) is designed to label cells at Ex/ Em =353/442 nm excited with a laser at 335 nm.

**Related assays** Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176741-200Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Tracking dye Blue", "number":"AB176741-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176741-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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