Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.
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Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Human, Mammals
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Human, Mammals
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.
The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.
The dye is designed to label cells at Ex/Em =410/450 nm excited with a violet laser at Ex=405 nm.
**Related assays**Review the to learn about kits to perform a , and .
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3 product images
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (ab176738)
HeLa cells fixed with formaldehyde and stained with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) (ab176738) in a Costar black wall/clear bottom 96-well plate.
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (ab176738)
Excitation and Emission spectra of CytoPainter Fixable Cell Viability Assay Kit.
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (ab176738)
Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (ab176738). Jurkat cells were treated and stained with Violet 450 Viability Dye, then fixed in 3.7% formaldehyde and analysed by flow cytometry. Live (Red), staurosporine treated (Green) and heat-treated (Blue) cells were distinguished with Pacific Blue channel.
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