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Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based (quantitative)
- Reactive species
Mammals
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Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Cell-based (quantitative)
- Reactive species
Mammals
- Assay Platform
Flow cytometer
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.
The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.
The dye is designed to label cells at Ex/Em =410/450 nm excited with a violet laser at Ex=405 nm.
**Related assays**Review the to learn about kits to perform a , and .
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