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AB176738

Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter

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Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.
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Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (AB176738)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (AB176738)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (ab176738). Jurkat cells were treated and stained with Violet 450 Viability Dye, then fixed in 3.7% formaldehyde and analysed by flow cytometry. Live (Red), staurosporine treated (Green) and heat-treated (Blue) cells were distinguished with Pacific Blue channel.

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (AB176738)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (AB176738)

HeLa cells fixed with formaldehyde and stained with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) (ab176738) in a Costar black wall/clear bottom 96-well plate.

Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (AB176738)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter (AB176738)

Excitation and Emission spectra of CytoPainter Fixable Cell Viability Assay Kit.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) | Cytopainter (ab176738) is used to evaluate the viability of mammalian cells by flow cytometry.

The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.

The dye is designed to label cells at Ex/Em =410/450 nm excited with a violet laser at Ex=405 nm.

**Related assays**Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176738-200Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176738-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Tracking dye Violet 450", "number":"AB176738-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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