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AB176745

Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter

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Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) | Cytopainter (ab176745) is used to evaluate the viability of mammalian cells by flow cytometry.
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Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (ab176745). Jurkat cells were treated and stained by Deep Red Viability Dye, then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue), staurosporine treated (Green) and heat-treated (Red) cells were distinguished with APC channel.

Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)

Jurkat cells were treated and stained with Tracking Dye Deep Red. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 630 nm / 660 nm (FL4). The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)

Excitation and Emission Spectra of CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) (ab176745)

Fluorescent Cell Imaging - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)
  • Fluorescent Cell Imaging

Supplier Data

Fluorescent Cell Imaging - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (AB176745)

Fluorescent imaging of HeLa cells fixed with formaldehyde and labeled with ab176745 in a black wall/ clear bottom 96 well plate

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) | Cytopainter (ab176745) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation. The approximate fluorescence excitation is 649 nm and emission maximium is 660 nm. The Excitation source is 633 nm.

The dye is designed to label cells at Ex/ Em = 649/660 nm. The Excitation source is 633 nm.

**Related assays** Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176745-200Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Tracking dye Deep Red", "number":"AB176745-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176745-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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