Fixable Cell Viability Assay Kit (Deep Red Fluorescence, for 633 nm excitation)
Be the first to review this product! Submit a review
|
(1 Publication)
- Flow Cyt
Supplier Data
Flow Cytometry - Fixable Cell Viability Assay Kit (Deep Red Fluorescence, for 633 nm excitation) (AB176745)
Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (ab176745). Jurkat cells were treated and stained by Deep Red Viability Dye, then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue), staurosporine treated (Green) and heat-treated (Red) cells were distinguished with APC channel.
- Flow Cyt
Supplier Data
Flow Cytometry - Fixable Cell Viability Assay Kit (Deep Red Fluorescence, for 633 nm excitation) (AB176745)
Jurkat cells were treated and stained with Tracking Dye Deep Red. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 630 nm / 660 nm (FL4). The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells
- Fluorescence Microscopy
Supplier Data
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Deep Red Fluorescence, for 633 nm excitation) (AB176745)
Excitation and Emission Spectra of CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) (ab176745)
- Fluorescent Cell Imaging
Supplier Data
Fluorescent Cell Imaging - Fixable Cell Viability Assay Kit (Deep Red Fluorescence, for 633 nm excitation) (AB176745)
Fluorescent imaging of HeLa cells fixed with formaldehyde and labeled with ab176745 in a black wall/ clear bottom 96 well plate
Reactivity data
Product details
Fixable Cell Viability Assay Kit (Deep Red Fluorescence, for 633 nm excitation) (ab176745) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation. The approximate fluorescence excitation is 649 nm and emission maximium is 660 nm. The Excitation source is 633 nm.
The dye is designed to label cells at Ex/ Em = 649/660 nm. The Excitation source is 633 nm.
**Related assays** Review the to learn about kits to perform a , and .
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
ACS chemical neuroscience 15:3473-3481 PubMed39307974
2024
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Assay Kits
AB176738
Fixable Cell Viability Assay Kit - (Blue Fluorescence for 405 nm excitation)
- 0
- 0
Assay Kits
AB176742
Fixable Cell Viability Assay Kit (Green Fluorescence, for 488 nm excitation)
- 0
- 0
Assay Kits
AB176739
Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation)
- 0
- 0
Assay Kits
AB176743
Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com