Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) | Cytopainter (ab176745) is used to evaluate the viability of mammalian cells by flow cytometry.
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Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) | Cytopainter (ab176745) is used to evaluate the viability of mammalian cells by flow cytometry.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) | Cytopainter (ab176745) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation. The approximate fluorescence excitation is 649 nm and emission maximium is 660 nm. The Excitation source is 633 nm.
The dye is designed to label cells at Ex/ Em = 649/660 nm. The Excitation source is 633 nm.
**Related assays** Review the to learn about kits to perform a , and .
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4 product images
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (ab176745)
Jurkat cells were treated and stained with Tracking Dye Deep Red. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 630 nm / 660 nm (FL4). The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells
Fluorescent Cell Imaging - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (ab176745)
Fluorescent imaging of HeLa cells fixed with formaldehyde and labeled with ab176745 in a black wall/ clear bottom 96 well plate
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (ab176745)
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (ab176745)
Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Deep Red Ex 633 nm) - Cytopainter (ab176745). Jurkat cells were treated and stained by Deep Red Viability Dye, then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue), staurosporine treated (Green) and heat-treated (Red) cells were distinguished with APC channel.
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