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AB176739

Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation)

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Abcam's  Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (ab176739) is used to evaluate the viability of mammalian cells by flow cytometry.
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Flow Cytometry - Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (AB176739)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (AB176739)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 405 nm) - CytoPainter (ab176739). Jurkat cells were treated and stained with V510 Viability Dye, then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue), staurosporine treated (Green) and heat-treated (Red) cells were distinguished with AmCyan channel.

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (AB176739)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (AB176739)

HeLa cells fixed with formaldehyde and stained with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 405 nm) (ab176739) in a Costar black wall/clear bottom 96-well plate.

Flow Cytometry - Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (AB176739)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (AB176739)

Excitation and Emission spectra of Tracking dye Violet 500 (ab176739).

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fixable Cell Viability Assay Kit (Green Fluorescence, for 405 nm excitation) (ab176739) is used to evaluate the viability of mammalian cells by flow cytometry.

The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.

The dye is designed to label cells at Ex/Em =408/512 nm excited with a violet laser at Ex=405 nm.

**Related assays** Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176739-200Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176739-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Tracking dye Violet 500", "number":"AB176739-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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