Fixable Cell Viability Assay Kit (Fluorometric - Green) | Cytopainter (ab176742) is used to evaluate the viability of mammalian cells by flow cytometry.
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Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Fixable Cell Viability Assay Kit (Fluorometric - Green) | Cytopainter (ab176742) is used to evaluate the viability of mammalian cells by flow cytometry.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Fixable Cell Viability Assay Kit (Fluorometric - Green) | Cytopainter (ab176742) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation. The approximate fluorescence excitation is 498 nm and emission maximium is 521 nm. The Excitation source is 488 nm.
**Related assays** Review the to learn about kits to perform a , and .
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4 product images
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 488 nm) - Cytopainter (ab176742)
Detection of Jurkat cell viability by CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green).
Detection of Jurkat cell viability using Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green) (ab176742). Jurkat cells were treated and stained with Tracking Dye Green. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 488 nm /520 nm (FL1). The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 488 nm) - Cytopainter (ab176742)
CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green).
Fluorescent imaging of HeLa cells using ab176742. HeLa cells were treated and stained with Tracking Dye Green. The cells were fixed in 3.7% formaldehyde and analyzed by fluorescence microscopy.
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 488 nm) - Cytopainter (ab176742)
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 488 nm) - Cytopainter (ab176742)
Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 488 nm) - Cytopainter (ab176742). Jurkat cells were treated and stained by Green Viability Dye, then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue), staurosporine treated (Green) and heat-treated (Dead, Red) cells were distinguished with FITC channel.
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