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AB176740

Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation)

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Abcam's Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.
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Flow Cytometry - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (AB176740)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (AB176740)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (ab176740). Jurkat cells were treated and stained with V550 Viability Dye, then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Red), staurosporine treated (Green) and heat-treated (Blue) cells were distinguished with Pacific Orange channel.

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (AB176740)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (AB176740)

HeLa cells fixed with formaldehyde and stained with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) (ab176740) in a Costar black wall/clear bottom 96-well plate.

Flow Cytometry - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (AB176740)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (AB176740)

Excitation and Emission spectra for CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) (ab176740).

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fixable Cell Viability Assay Kit (Orange Fluorescence, for 405 nm excitation) (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.

This particular kit is designed to uniformly label fixed mammalian cells in blue fluorescence for flow cytometry applications with violet laser excitation. The kit uses a proprietary blue fluorescent dye that is more fluorescent upon bonding to cellular components. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.

The dye is designed to label cells at Ex/Em = 398/550 nm excited with a violet laser at 405 nm.

**Related assays** Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176740-200Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176740-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Tracking dye Violet 550", "number":"AB176740-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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