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Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) | Cytopainter (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.

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Images

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (AB176740), expandable thumbnail
  • Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (AB176740), expandable thumbnail

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based (quantitative)
Reactive species
Mammals

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What's included?

200 Test
Components
DMSO
1 x 200 µL
Tracking dye Violet 550
1 x 1 Vial

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Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) | Cytopainter (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based (quantitative)
Reactive species
Mammals
Assay Platform
Flow cytometer

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) | Cytopainter (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.

This particular kit is designed to uniformly label fixed mammalian cells in blue fluorescence for flow cytometry applications with violet laser excitation. The kit uses a proprietary blue fluorescent dye that is more fluorescent upon bonding to cellular components. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.

The dye is designed to label cells at Ex/Em = 398/550 nm excited with a violet laser at 405 nm.

**Related assays**Review the to learn about kits to perform a , and .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (ab176740), expandable thumbnail

    Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (ab176740)

    HeLa cells fixed with formaldehyde and stained with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) (ab176740) in a Costar black wall/clear bottom 96-well plate.

  • Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (ab176740), expandable thumbnail

    Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (ab176740)

    Spectrum for ab176740.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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