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AB176743

Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation)

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Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (ab176743) is used to evaluate the viability of mammalian cells by flow cytometry.
3 Images
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (AB176743)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (AB176743)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 488 nm) - Cytopainter (ab176743). Jurkat cells were heat- treated at 60°C or left untreated, and stained by Orange Viability Dye. Live and heat-treated cells were imaged with fluorescence microscope using TRITC filter.

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (AB176743)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (AB176743)

Excitation and Emission Spectra of CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Orange) (ab176743)

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (AB176743)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (AB176743)

Fluorescent imaging of HeLa cells fixed with formaldehyde and labeled with CytoPainter Fixable Cell Viability Assay Kit (Fluorometric-Orange) (ab176743) in a black wall/ clear bottom 96 well plate.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Fluorescence Microscopy": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Fixable Cell Viability Assay Kit (Orange Fluorescence, for 488 nm or 561 nm excitation) (ab176743) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.

The dye is designed to label cells at Ex/ Em = 547/573 nm. The Excitation source is 488nm or 561 nm.

**Related assays** Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176743-200Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Tracking dye Orange", "number":"AB176743-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176743-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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