Fixable Cell Viability Assay Kit (Fluorometric - Orange) | Cytopainter (ab176743) is used to evaluate the viability of mammalian cells by flow cytometry.
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Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Fluorescence Microscopy | Reactivity Reacts | Dilution info - | Notes - |
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Fixable Cell Viability Assay Kit (Fluorometric - Orange) | Cytopainter (ab176743) is used to evaluate the viability of mammalian cells by flow cytometry.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Cell-based (quantitative)
- Reactive species
- Mammals, Human
- Assay Platform
- Flow cytometer
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Fixable Cell Viability Assay Kit (Fluorometric - Orange) | Cytopainter (ab176743) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.
The dye is designed to label cells at Ex/ Em = 547/573 nm. The Excitation source is 488nm or 561 nm.
**Related assays** Review the to learn about kits to perform a , and .
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3 product images
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 488 nm) - Cytopainter (ab176743)
Fluorescent imaging of HeLa cells fixed with formaldehyde and labeled with CytoPainter Fixable Cell Viability Assay Kit (Fluorometric-Orange) (ab176743) in a black wall/ clear bottom 96 well plate.
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 488 nm) - Cytopainter (ab176743)
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 488 nm) - Cytopainter (ab176743)
Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 488 nm) - Cytopainter (ab176743). Jurkat cells were heat- treated at 60°C or left untreated, and stained by Orange Viability Dye. Live and heat-treated cells were imaged with fluorescence microscope using TRITC filter.
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