Fixable Cell Viability Assay Kit (Fluorometric - Red Ex 561 nm) - Cytopainter
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(2 Publications)
- Fluorescence Microscopy
Supplier Data
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Red Ex 561 nm) - Cytopainter (AB176744)
Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Red Ex 561 nm) - Cytopainter (ab176744). Jurkat cells were heat- treated at 60°C or left untreated, and stained by Red Viability Dye. Live and heat-treated cells were imaged with fluorescence microscope using Texas Red filter.
- Fluorescence Microscopy
Supplier Data
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Fluorometric - Red Ex 561 nm) - Cytopainter (AB176744)
HeLa cells fixed with formaldehyde and labeled with CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Red) (ab176744) in a black wall/ clear bottom 96 well plate.
- Flow Cyt
Supplier Data
Flow Cytometry - Fixable Cell Viability Assay Kit (Fluorometric - Red Ex 561 nm) - Cytopainter (AB176744)
Excitation and Emission spectra for CytoPainter Fixable Cell Viability Assay Kit (Fluorometric-Red) (ab176744).
Reactivity data
Product details
Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Red) (ab176744) is used to evaluate the viability of mammalian cells by flow cytometry.
The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.
The dye is designed to label cells at Ex/ Em = 583/603 nm. The Excitation source is 561 nm.
**Related assays** Review the to learn about kits to perform a , and .
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Publications (2)
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Molecular & cellular proteomics : MCP 22:100653 PubMed37739344
2023
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Nature communications 6:6582 PubMed25791197
2015
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Species
Unspecified reactive species
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