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AB176744

Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation)

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(3 Publications)

Abcam's Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (ab176744) is used to evaluate the viability of mammalian cells by flow cytometry.
3 Images
Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (AB176744)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (AB176744)

Detection of Jurkat cell viability by Fixable Cell Viability Assay Kit (Fluorometric - Red Ex 561 nm) - Cytopainter (ab176744). Jurkat cells were heat- treated at 60°C or left untreated, and stained by Red Viability Dye. Live and heat-treated cells were imaged with fluorescence microscope using Texas Red filter.

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (AB176744)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (AB176744)

HeLa cells fixed with formaldehyde and labeled with CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Red) (ab176744) in a black wall/ clear bottom 96 well plate.

Flow Cytometry - Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (AB176744)
  • Flow Cyt

Supplier Data

Flow Cytometry - Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (AB176744)

Excitation and Emission spectra for CytoPainter Fixable Cell Viability Assay Kit (Fluorometric-Red) (ab176744).

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Flow cytometer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Fluorescence Microscopy": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fixable Cell Viability Assay Kit (Red Fluorescence, for 561 nm excitation) (ab176744) is used to evaluate the viability of mammalian cells by flow cytometry.

The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 fold and can be completely preserved after fixation.

The dye is designed to label cells at Ex/ Em = 583/603 nm. The Excitation source is 561 nm.

**Related assays** Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab176744-200Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"DMSO", "number":"AB176744-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Tracking dye Red", "number":"AB176744-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadq6077 PubMed40267187

2025

Loss of intracellular ATP affects axoplasmic viscosity and pathological protein aggregation in mammalian neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Laurent Guillaud,Anna Garanzini,Sarah Zakhia,Sandra De la Fuente,Dimitar Dimitrov,Susan Boerner,Marco Terenzio

Molecular & cellular proteomics : MCP 22:100653 PubMed37739344

2023

FMRP Long-Range Transport and Degradation Are Mediated by Dynlrb1 in Sensory Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Emad El-Agamy,Laurent Guillaud,Keiko Kono,Yibo Wu,Marco Terenzio

Nature communications 6:6582 PubMed25791197

2015

Transfer hydrogenation catalysis in cells as a new approach to anticancer drug design.

Applications

Unspecified application

Species

Unspecified reactive species

Joan J Soldevila-Barreda,Isolda Romero-Canelón,Abraha Habtemariam,Peter J Sadler
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