Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710) is an assay where FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- iScience 27:1092762024Chronic stress alters hepatic metabolism and thermodynamic respiratory efficiency affecting epigenetics in C57BL/6 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Nikolic,Pia Fahlbusch,Nele-Kathrien Riffelmann,Natalie Wahlers,Sylvia Jacob,Sonja Hartwig,Ulrike Kettel,Martina Schiller,Matthias Dille,Hadi Al-Hasani,Jörg Kotzka,Birgit KnebelPubMed 38450153
- Molecular biology of the cell 32:br102021Riboflavin transporter SLC52A1, a target of p53, suppresses cellular senescence by activating mitochondrial complex II.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaiki Nagano,Yuto Awai,Shione Kuwaba,Taiichi Osumi,Kentaro Mio,Tetsushi Iwasaki,Shinji KamadaPubMed 34524871
- Nature communications 11:20822020LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas K Vinckier,Nisha A Patel,Ryan J Geusz,Allen Wang,Jinzhao Wang,Ileana Matta,Austin R Harrington,Matthew Wortham,Nichole Wetton,Jianxun Wang,Ulupi S Jhala,Michael G Rosenfeld,Christopher W Benner,Hung-Ping Shih,Maike SanderPubMed 32350257
- eLife 9:2020Loss of flavin adenine dinucleotide (FAD) impairs sperm function and male reproductive advantage in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-An Yen,Dana L Ruter,Christian D Turner,Shanshan Pang,Sean P CurranPubMed 32022684
- PloS one 14:e02267572019Autofluorescence spectroscopy in redox monitoring across cell confluencies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerrick Yong et. al.PubMed 31851724
- Cell chemical biology 26:745-755.e72019Multi-metal Restriction by Calprotectin Impacts De Novo Flavin Biosynthesis in Acinetobacter baumannii.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiefei Wang et. al.PubMed 30905682
- Life science alliance 2:2019d-amino acid oxidase promotes cellular senescence via the production of reactive oxygen species.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaiki Nagano et. al.PubMed 30659069
- Clinical and experimental dental research 4:174-1812018The luminance ratio of autofluorescence in a xenograft mouse model is stable through tumor growth stages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShigeki Sumi,Naoki Umemura,Makoto Adachi,Takahisa Ohta,Kosuke Naganawa,Harumi Kawaki,Eiji Takayama,Nobuo Kondoh,Shinichiro SumitomoPubMed 30386639
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Tissue Lysate, Plasma, Cell culture media, Serum, Cell Lysate
- Assay type
Enzyme activity
- Reactive species
Mammals
- Sensitivity
< 1 nmol/well
What's included?
Recommended products
Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710) is an assay where FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Tissue Lysate, Plasma, Cell culture media, Serum, Cell Lysate
- Assay type
Enzyme activity
- Reactive species
Mammals
- Assay Platform
Microplate reader
- Sensitivity
< 1 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710) is an assay where FAD functions as the cofactor of an oxidase which catalyze the formation of a product that reacts with OxiRed probe generating color and fluorescence. FAD can be detected by either colorimetric (OD=570 nm) or fluorometric (Ex/Em=535/587 nm) methods. The kit provides a rapid, simple, ultra-sensitive, and reliable test suitable for high throughput assay of FAD. The lower limit of detection is less than 1 nM FAD.
FAD assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader until absorbance reaches 1.8 at 570 nm (or stop before then)
This product is manufactured by BioVision, an Abcam company and was previously called K357 FAD Colorimetric/Fluorometric Assay Kit. K357-100 is the same size as the 100 test size of ab204710.
Flavin Adenine Dinucleotide (FAD) is a redox cofactor which plays an important role in metabolism. FAD exists in different redox states and cycles between FAD, FADH and FADH2. The primary sources of reduced FAD in eukaryotic metabolism are the citric acid cycle and the beta oxidation reaction pathways.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Flavin Adenine Dinucleotide (FAD) sometimes referred to as flavins or FAD flavin is a coenzyme that plays a mechanical role by accepting and donating electrons in various biochemical reactions. It has a molecular mass of about 785.55 g/mol. FAD is widely expressed in cells particularly in the mitochondria where it participates in important redox reactions necessary for cellular energy production. The coenzyme is essential in processes that convert nutrients into energy facilitating cellular metabolism.
- Biological function summary
FAD functions to transfer electrons in key metabolic reactions. It often operates as part of larger enzyme complexes like succinate dehydrogenase which is involved in the citric acid cycle and electron transport chain. In these complexes FAD acts as a redox-active cofactor cycling between its oxidized form (FAD) and its reduced form (FADH2 or FAD/FADH). This conversion is essential for maintaining the flow of electrons during cellular respiration enabling efficient ATP production.
- Pathways
FAD is a critical component of the citric acid cycle and the electron transport chain two major metabolic pathways. In the citric acid cycle FADH2 is generated during the oxidation of succinate to fumarate aiding in the transfer of electrons to the electron transport chain. Along this pathway FAD interacts with proteins such as succinate dehydrogenase and other flavoproteins ensuring the efficient generation of ATP. These pathways highlight FAD's role in sustaining cellular respiration and energy homeostasis.
- Associated diseases and disorders
Disruptions in FAD-related enzymes can contribute to metabolic disorders like Multiple Acyl-CoA Dehydrogenase Deficiency (MADD) where FAD-linked enzyme deficiencies lead to impaired fatty acid oxidation. Additionally FAD's role in energy production implicates it in mitochondrial diseases with conditions such as Leigh syndrome arising from defects in FAD-dependent pathways and related proteins like succinate dehydrogenase. These associations highlight the importance of FAD as a cofactor in maintaining metabolic and mitochondrial health.
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2 product images
Functional Studies - Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710)
Typical FAD Standard Curve (Fluorometric Assay).
Functional Studies - Flavin Adenine Dinucleotide (FAD) Assay Kit (ab204710)
Typical FAD Standard Curve (Colorimetric Assay).
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