Fluo-4 Assay Kit (Calcium) (ab228555) provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization.
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- JCI insight 8:2023Specific regulation of mechanical nociception by Gβ5 involves GABA-B receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMritunjay Pandey,Jian-Hua Zhang,Poorni R Adikaram,Claire Kittock,Nicole Lue,Adam Awe,Katherine Degner,Nirmal Jacob,Jenna Staples,Rachel Thomas,Allison B Kohnen,Sundar Ganesan,Juraj Kabat,Ching-Kang Chen,William F SimondsPubMed 37219953
- Theranostics 11:7379-73902021TRPV2-spike protein interaction mediates the entry of SARS-CoV-2 into macrophages in febrile conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinrui Xu,Yuquan Yang,Zhaoyuan Hou,Hao Jia,Yujiong WangPubMed 34158856
- Cell cycle (Georgetown, Tex.) 19:3639-36492020Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhu Zeng,Haixin Ma,Jing Chen,Nina Huang,Yudan Zhang,Yufei Su,Huifang ZhangPubMed 33323026
- Cell communication and signaling : CCS 18:862020Regulators of G-protein signaling, RGS2 and RGS4, inhibit protease-activated receptor 4-mediated signaling by forming a complex with the receptor and Gα in live cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukeyoung Kim et. al.PubMed 32517689
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
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Target data
Alternative names
Ca++, Ca2+
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Fluo-4 Assay Kit (Calcium) (ab228555) provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization.
Key facts
- Detection method
- Fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Fluo-4 Assay Kit (Calcium) (ab228555) provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. The kit offers an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels.
The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
This product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR™, FDSS, BMG NOVOstar™, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fluo-4 AM which can cross cell membrane. Once inside the cell, the lipohilic blocking groups of Fluo-4 AM are cleaved by non-specific cell esterase, resulting in a negatively charged Fluo-4 dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-4.
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Cellular Activity - Fluo-4 Assay Kit (Calcium) (ab228555)
ATP dose response was measured in CHO-K1 cells with Fluo-4 Assay Kit (Calcium).
CHO-K1 cells were seeded overnight at 60,000 cells/100 μL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 μL of dye-loading solution using the Fluo-4 Assay Kit (Calcium) for 1 hour at 37oC, and then at room temperature for another 15 minutes. ATP (50μL/well) was added by Flexstation 3 to achieve the final indicated concentrations.
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