AB228555

Fluo-4 Assay Kit (Calcium)

Name: Fluo-4 Assay Kit (Calcium) (ab228555) | Abcam
Brand: Abcam Limited
SKU: AB228555
Price: 565 USD
Availability: InStock

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(5 Publications)

Fluo-4 Assay Kit (Calcium) (ab228555) provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization.

View Alternative Names

Ca++, Ca2+

1 Images

Cellular Activity - Fluo-4 Assay Kit (Calcium) (AB228555)

Cellular Activity

Supplier Data

Cellular Activity - Fluo-4 Assay Kit (Calcium) (AB228555)

ATP dose response was measured in CHO-K1 cells with Fluo-4 Assay Kit (Calcium).

CHO-K1 cells were seeded overnight at 60,000 cells/100 μL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 μL of dye-loading solution using the Fluo-4 Assay Kit (Calcium) for 1 hour at 37^oC, and then at room temperature for another 15 minutes. ATP (50μL/well) was added by Flexstation 3 to achieve the final indicated concentrations.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay Platform

Microplate reader

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Product details

Fluo-4 Assay Kit (Calcium) (ab228555) provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. The kit offers an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels.

The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.

This product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR™, FDSS, BMG NOVOstar™, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fluo-4 AM which can cross cell membrane. Once inside the cell, the lipohilic blocking groups of Fluo-4 AM are cleaved by non-specific cell esterase, resulting in a negatively charged Fluo-4 dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-4.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

See full target information Calcium

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

F1000Research 12:47 PubMed39816762

2025

Regulation of MAP2, GFAP, and calcium in the CA3 Region Following Kainic Acid Exposure to organotypic hippocampal slice culture.

Applications

Unspecified application

Species

Unspecified reactive species

Machlusil Husna,Kusworini Handono,Hidayat Sujuti,Aulanni'am Aulanni'am,Ettie Rukmigarsari

JCI insight 8: PubMed37219953

2023

Specific regulation of mechanical nociception by Gβ5 involves GABA-B receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Mritunjay Pandey,Jian-Hua Zhang,Poorni R Adikaram,Claire Kittock,Nicole Lue,Adam Awe,Katherine Degner,Nirmal Jacob,Jenna Staples,Rachel Thomas,Allison B Kohnen,Sundar Ganesan,Juraj Kabat,Ching-Kang Chen,William F Simonds

Theranostics 11:7379-7390 PubMed34158856

2021

TRPV2-spike protein interaction mediates the entry of SARS-CoV-2 into macrophages in febrile conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Jinrui Xu,Yuquan Yang,Zhaoyuan Hou,Hao Jia,Yujiong Wang

Cell cycle (Georgetown, Tex.) 19:3639-3649 PubMed33323026

2020

Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Zeng,Haixin Ma,Jing Chen,Nina Huang,Yudan Zhang,Yufei Su,Huifang Zhang

Cell communication and signaling : CCS 18:86 PubMed32517689

2020

Regulators of G-protein signaling, RGS2 and RGS4, inhibit protease-activated receptor 4-mediated signaling by forming a complex with the receptor and Gα in live cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yukeyoung Kim,Sungho Ghil

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Fluo-4 Assay Kit (Calcium)

Cellular Activity - Fluo-4 Assay Kit (Calcium) (AB228555)

Key facts

Detection method

Sample types

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

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