Skip to main content

Fluo-8 Calcium Assay Kit - Medium Removal (ab112128) is a fluorescence-based assay for detecting intracellular calcium mobilization.

Be the first to review this product! Submit a review

Images

Functional Studies - Fluo-8 Calcium Assay Kit - Medium Removal (AB112128), expandable thumbnail

Publications

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based
Reactive species
Mammals

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Alternative names

Ca++, Ca2+

What's included?

1 x 96 Tests
Components
10X Pluronic® F127 Plus
1 x 1 mL
Fluo-8
1 x 1 Vial
HHBS
1 x 9 mL

Recommended products

Fluo-8 Calcium Assay Kit - Medium Removal (ab112128) is a fluorescence-based assay for detecting intracellular calcium mobilization.

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based
Reactive species
Mammals
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's Fluo-8 Calcium Assay Kit - Medium Removal (ab112128) is a fluorescence-based assay for detecting intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fluo-8 which can cross the cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-8 are cleaved by an esterase, resulting in a negatively charged fluorescent dye that stays inside the cell. Its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increases the fluorescence of Fluo-8. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement make Fluo-8 the brightest green calcium indicator available in the marker, and it is an ideal tool for the measurement of cellular calcium through HTS screening.

ab112128 provides an optimized assay method for monitoring the G-protein-coupled receptors and calcium channels using HTS instrumentation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

Fluo-8 products available

Fluo-8 Calcium Flux Assay Kits are available in No Wash and Medium Removal variants:
- No wash kit Fluo-8 Calcium Flux Assay Kit Fluo-8 Calcium Flux Assay Kit - No Wash ab112129 is generally preferred as the workflow is simpler. It is also recommended for cell lines that are only weakly adherent and risk losing adherence when washing.

- Medium removal kit Fluo-8 Calcium Flux Assay Kit ab112128 is recommended for the testing of compounds, receptors and calcium channels that are sensitive to the presence of cell culture media or serum. Alternatively, for cells that can tolerate it, consider using a low serum % in culture media and use No Wash Fluo-8 kit Fluo-8 Calcium Flux Assay Kit - No Wash ab112129.

Fluo-8 is also available as a standalone biochemical Fluo-8 AM, green fluorescent calcium binding dye ab142773.

This product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR™, FDSS, BMG NOVOstar™, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.

If you would like to quantify calcium concentration in vitro using cell extracts, we recommend using Calcium Detection Kit (Colorimetric) (Calcium Assay Kit (Colorimetric) ab102505) or Calcium Quantification Assay Kit (Calcium Assay kit (Fluorometric) ab112115), as they provide stock standards for comparison.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - Fluo-8 Calcium Assay Kit - Medium Removal (ab112128), expandable thumbnail

    Functional Studies - Fluo-8 Calcium Assay Kit - Medium Removal (ab112128)

    Carbachol Dose Response was measured in HEK293 cells with ab112128 and a Fluo-4 assay kit. HEK293 cells were seeded overnight at 40,000 cells/100 µL/well in a black wall/clear bottom 96-well plate. The growth medium was removed, and the cells were incubated with 100 µL of dye-loading solution using ab112128 or the Fluo-4 kit for 1 hour at room temperature. Carbachol (25 µL/well) was added to achieve the final indicated concentrations. The EC50 of ab112128 is about 1.2 µM.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com