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AB272524

Formaldehyde Assay Kit

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(1 Publication)

Formaldehyde Assay Kit (ab272524) is a mix-and-read assay with a single 30-min incubation at room temperature. Readout on any fluorometric (Ex/Em 370/470 nm) plate reader.
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Functional Studies - Formaldehyde Assay Kit (AB272524)
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Supplier Data

Functional Studies - Formaldehyde Assay Kit (AB272524)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Fluorescent

Sample types

Urine, Plasma, Serum, Other biological fluids, Cell Lysate

Results type

Quantitative

Sensitivity

= 1.5 µM

Assay Platform

Microplate

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Product details

Formaldehyde Assay Kit (ab272524) provides a convenient fluorimetric means to measure formaldehyde in biological samples.

In the Formaldehyde assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia. The resulting fluorescent product is then quantified fluorimetrically (Ex/Em 370/470nm)

Formaldehyde assay protocol summary
Prepare all reagents and samples as instructed.
- Add samples and standards to wells
- Add working reagent
- Incubate for 30 minutes at RT protected from light.
- analyse on plate reader

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Formaldehyde also known as methanal is the simplest form of aldehyde with a molecular mass of 30.03 g/mol. It naturally occurs as a gas and dissolves in water to form formalin. Inside living organisms formaldehyde is endogenously produced and can be expressed in various tissues including liver and kidneys. Formaldehyde influences several cellular processes by modifying proteins and nucleic acids through the formation of cross-links affecting their function and stability.
Biological function summary

Formaldehyde plays a significant role in cell metabolism and gene regulation. It participates in the one-carbon cycle as part of the detoxification system. The enzyme formaldehyde dehydrogenase aids in its conversion to formate which cells can utilize for nucleotide synthesis and methylation reactions. This association makes formaldehyde a part of the folate-mediated one-carbon metabolic pathway essential for DNA synthesis and repair.

Pathways

Formaldehyde is involved in metabolic processes like the methionine and serine cycles which are critical for amino acid interconversion and methyl group donation. It shares significant interaction with proteins such as S-adenosylmethionine (SAM) known for its role as a major methyl donor in various biological reactions. These interactions highlight formaldehyde's importance in maintaining cellular homeostasis and epigenetic regulation.

Formaldehyde exposure and metabolism have been linked to certain types of cancers and neurodegenerative diseases. Its accumulation can lead to DNA and protein damage contributing to carcinogenesis and diseases like Alzheimer's where tangles and plaques play a major role. Proteins such as amyloid-beta which are involved in these disorders can be affected by formaldehyde-induced modifications further implicating its influence in disease pathogenesis.
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Product protocols

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Target data

See full target information Formaldehyde
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology 12: PubMed37626940

2023

High-Throughput Assay of Cytochrome P450-Dependent Drug Demethylation Reactions and Its Use to Re-Evaluate the Pathways of Ketamine Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Nadezhda Y Davydova,David A Hutner,Kari A Gaither,Dilip Kumar Singh,Bhagwat Prasad,Dmitri R Davydov
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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