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AB111748

Formate Assay Kit

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(5 Publications)

Formate Assay Kit ab111748 is a simple and direct colorimetric assay for detecting formate in biological samples.
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Functional Studies - Formate Assay Kit (AB111748)
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Supplier Data

Functional Studies - Formate Assay Kit (AB111748)

Example of standard curve obtained using ab111748

Schematic Diagram - Formate Assay Kit (AB111748)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Formate Assay Kit (AB111748)

Representative image of Formate Assay Kit ab111748

Components shown from left to right :

- Formate Standard

- Developer Solution III

- Enzyme Mix IX

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Assay time

1h

Assay Platform

Microplate reader

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Product details

Formate Assay Kit ab111748 is a simple and direct colorimetric assay for detecting formate in biological samples.

Formate assay principle
In the formate assay protocol, formate is oxidized to generate a product resulting in color formation (450 nm) proportional to formate concentration. The amount of formate in the sample is then determined by comparing the color to a standard curve.

Formate assay protocol summary
- Add samples, standards and positive control to wells
- Add formate reagent
- Incubate for 60 minutes at at 37°C.
- analyse with plate reader.

Other Notes
This product was previously called K653 Biovision Formate Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab111748-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Enzyme Mix IX", "number":"AB111748-CMP02", "productcode":"" }, { "size":"1 x 0.1 mL", "name":"Formate Standard", "number":"AB111748-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB111748-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB111748-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Formate a simple anion derived from formic acid plays a significant role in metabolic processes. Known by its systematic name methanoate formate is a small molecule with a molar mass of 46.03 g/mol. It is found across various tissues where it participates in one-carbon metabolism. Cells generate formate through the breakdown of certain amino acids a pathway present in both mitochondria and cytosol. These sites of expression indicate the key involvement of formate in cellular energy dynamics.
Biological function summary

Formate acts as an important intermediary in one-carbon units transfer. It participates in the folate cycle where it serves as a donor of one-carbon units in the synthesis of purines and thymidine. Formate does not work alone; it often functions as part of a complex with other molecules involved in one-carbon metabolism pathways. The transfer of one-carbon units is essential for nucleotide biosynthesis and has implications in cellular proliferation and repair processes.

Pathways

Formate takes part actively in the folate and methionine cycles. These pathways play roles in cellular growth and methylation processes. Related proteins include methylenetetrahydrofolate dehydrogenase which facilitates formate's conversion into other active metabolic forms. Formate's role connects with pivotal cellular pathways that maintain homeostasis and growth implicating its significance in the broader metabolic network.

Formate's metabolism links to conditions such as folate deficiency and cancer. Deficient folate levels may lead to impaired nucleotide synthesis with consequences for DNA repair mechanisms while altered formate metabolism is observed in certain cancers hinting at increased proliferation requirements. Proteins like dihydrofolate reductase which interacts with folate metabolism relate formate's biological activity to these disease states. Understanding the roles of formate and its associated proteins can offer insights into potential therapeutic targets.
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Product protocols

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Target data

See full target information Formate
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Metabolites 14: PubMed38248848

2024

Dysbiotic Vaginal Microbiota Induces Preterm Birth Cascade via Pathogenic Molecules in the Vagina.

Applications

Unspecified application

Species

Unspecified reactive species

AbuZar Ansari,Young-Ah You,Gain Lee,Soo Min Kim,Sun Wha Park,Young Min Hur,Young Ju Kim

Applied and environmental microbiology 89:e0147423 PubMed37966269

2023

NADP or CO reduction by -encoded hydrogenase through interaction with formate dehydrogenase 3 in the hyperthermophilic archaeon NA1.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-In Yang,Hae-Chang Jung,Hyun-Myung Oh,Bo Gyoung Choi,Hyun Sook Lee,Sung Gyun Kang

Frontiers in aging neuroscience 14:1046066 PubMed36533170

2022

Systematic evaluation of urinary formic acid as a new potential biomarker for Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Wang,Ying Wang,Jinhang Zhu,Yihui Guan,Fang Xie,Xiao Cai,Jiale Deng,Yan Wei,Rongqiao He,Zhuo Fang,Qihao Guo

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34244426

2021

p53 deficiency induces MTHFD2 transcription to promote cell proliferation and restrain DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Gen Li,Jun Wu,Le Li,Peng Jiang

Cell death & disease 11:310 PubMed32366892

2020

Formate induces a metabolic switch in nucleotide and energy metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Kristell Oizel,Jacqueline Tait-Mulder,Jorge Fernandez-de-Cossio-Diaz,Matthias Pietzke,Holly Brunton,Sergio Lilla,Sandeep Dhayade,Dimitri Athineos,Giovanny Rodriguez Blanco,David Sumpton,Gillian M Mackay,Karen Blyth,Sara R Zanivan,Johannes Meiser,Alexei Vazquez
View all publications
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