In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (? = 450 nm) proportional to formate concentration.
Colorimetric
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
Quantitative
1h
In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (? = 450 nm) proportional to formate concentration.
Colorimetric
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
Quantitative
1h
Microplate reader
Blue Ice
-20°C
-20°C
-20°C
In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.
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This product is manufactured by BioVision, an Abcam company and was previously called K653 Formate Colorimetric Assay Kit. K653-100 is the same size as the 100 test size of ab111748.
Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.
This supplementary information is collated from multiple sources and compiled automatically.
Formate a simple anion derived from formic acid plays a significant role in metabolic processes. Known by its systematic name methanoate formate is a small molecule with a molar mass of 46.03 g/mol. It is found across various tissues where it participates in one-carbon metabolism. Cells generate formate through the breakdown of certain amino acids a pathway present in both mitochondria and cytosol. These sites of expression indicate the key involvement of formate in cellular energy dynamics.
Formate acts as an important intermediary in one-carbon units transfer. It participates in the folate cycle where it serves as a donor of one-carbon units in the synthesis of purines and thymidine. Formate does not work alone; it often functions as part of a complex with other molecules involved in one-carbon metabolism pathways. The transfer of one-carbon units is essential for nucleotide biosynthesis and has implications in cellular proliferation and repair processes.
Formate takes part actively in the folate and methionine cycles. These pathways play roles in cellular growth and methylation processes. Related proteins include methylenetetrahydrofolate dehydrogenase which facilitates formate's conversion into other active metabolic forms. Formate's role connects with pivotal cellular pathways that maintain homeostasis and growth implicating its significance in the broader metabolic network.
Formate's metabolism links to conditions such as folate deficiency and cancer. Deficient folate levels may lead to impaired nucleotide synthesis with consequences for DNA repair mechanisms while altered formate metabolism is observed in certain cancers hinting at increased proliferation requirements. Proteins like dihydrofolate reductase which interacts with folate metabolism relate formate's biological activity to these disease states. Understanding the roles of formate and its associated proteins can offer insights into potential therapeutic targets.
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Example of standard curve obtained using ab111748
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