FRAP Assay Kit / Ferric Reducing Antioxidant Power Assay Kit ab234626 is used to measure the antioxidant capacity of biological samples. Readout on any colorimetric (594 nm) plate reader.
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- Antioxidants (Basel, Switzerland) 13:2024Obesogenic Diet in Mice Leads to Inflammation and Oxidative Stress in the Mother in Association with Sex-Specific Changes in Fetal Development, Inflammatory Markers and Placental Transcriptome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlejandro A Candia,Samantha C Lean,Cindy X W Zhang,Daniel R McKeating,Anna Cochrane,Edina Gulacsi,Emilio A Herrera,Bernardo J Krause,Amanda N Sferruzzi-PerriPubMed 38671859
- Cancers 15:2023Discovery of Novel Bioactive Tanshinones and Carnosol Analogues against Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel A González-Cardenete,Natalia González-Zapata,Lucinda Boyd,Fatima RivasPubMed 36831660
- ACS omega 6:35334-353412021Efficient Citrus () Byproduct Extract-Based Approach for WiKim31 Shelf-Life Extension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHo Myeong Kim,Seul-Gi Jeong,In Min Hwang,Hae Woong ParkPubMed 34984265
- International journal of molecular sciences 22:2021Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSydney L Lane,Jason C Parks,Jennifer E Russ,Shaihla A Khan,William B Schoolcraft,Ye Yuan,Mandy G Katz-JaffePubMed 34884824
- Antioxidants (Basel, Switzerland) 10:2021UPLC-QTOF-MS/MS and GC-MS Characterization of Phytochemicals in Vegetable Juice Fermented Using Lactic Acid Bacteria from Kimchi and Their Antioxidant Potential.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoeun Lee,Jung Hee Song,Eun Ji Choi,Ye-Rang Yun,Ki Won Lee,Ji Yoon ChangPubMed 34829632
- International journal of molecular sciences 22:2021Phenolic Profiles of Red Wine Relate to Vascular Endothelial Benefits Mediated by SIRT1 and SIRT6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunzia D'Onofrio,Elisa Martino,Giuseppina Chianese,Francesca Coppola,Luigi Picariello,Luigi Moio,Maria Luisa Balestrieri,Angelita Gambuti,Martino ForinoPubMed 34073604
- ACS omega 6:8171-81782021Production, Characterization, and Antioxidant Activities of an Exopolysaccharide Extracted from Spent Media Wastewater after WiKim32 Fermentation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIn Seong Choi,Seung Hee Ko,Mo Eun Lee,Ho Myeong Kim,Jung Eun Yang,Seul-Gi Jeong,Kwang Ho Lee,Ji Yoon Chang,Jin-Cheol Kim,Hae Woong ParkPubMed 33817476
- International journal of applied & basic medical research 10:110-1162020Improvement of on Morphine Toxicity in Prefrontal Cortex in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Reza Salahshoor,Amir Abdolmaleki,Cyrus Jalili,Arash Ziapoor,Shiva RoshankhahPubMed 32566527
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Quantitative
- Sensitivity
- = 0.2 mM
What's included?
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FRAP Assay Kit / Ferric Reducing Antioxidant Power Assay Kit ab234626 is used to measure the antioxidant capacity of biological samples. Readout on any colorimetric (594 nm) plate reader.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Quantitative
- Assay Platform
- Microplate reader
- Sensitivity
- = 0.2 mM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
FRAP Assay Kit ab234626 provides a quick, sensitive and easy way for measuring the antioxidant capacity of various biological samples. FRAP assay stands for Ferric Reducing Antioxidant Power Assay.
The FRAP assay is high-throughput, adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.
FRAP assay principle
The assay measures the antioxidant potential in samples through the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the samples. Following the reduction of the ferric iron, a blue color develops that is read colorimetrically at 594 nm.
The antioxidant potential of samples is determined using a ferrous iron standard curve and results are expressed as Fe2+ equivalents (μM) or FRAP value.
FRAP assay protocol summary
- add samples and standards to wells
- add reaction mix
- measure absorbance at 594 nm in kinetic mode for 60 min at 37°C
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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3 product images
Functional Studies - FRAP Assay Kit (Ferric Reducing Antioxidant Power Assay) (ab234626)
Absorbance readings for positive control and 10 μl diluted solutions of grape juice (1:50 dilution with dH2O), strawberry methanolic extract (extract made from 50 mg of freeze-dried strawberries in 5 ml of extraction solvent and final solution diluted 1:80 times with dH2O) and green tea (brewed for 5 minutes and diluted 1:120 times with dH2O).
Functional Studies - FRAP Assay Kit (Ferric Reducing Antioxidant Power Assay) (ab234626)
Ferrous standard curve.
Functional Studies - FRAP Assay Kit (Ferric Reducing Antioxidant Power Assay) (ab234626)
Absorbance readings for mM Ferrous equivalents or FRAP of grape juice, strawberry and green tea.
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