Free Fatty Acid Assay Kit - Quantification

• Biochemical assay

Lab

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

Diagram showing the principles of the Free Fatty Acid assay method.

• Biochemical assay

PubMed

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

Triglyceride assay kit ab65336 and free fatty acid assay kit ab65341 used with heart tissue.

Han et al. used Triglyceride assay kit ab65336 and Free Fatty Acid assay kit ab65341 to investigate cardiac abnormalities in high fat diet fed mice when researching the role of the long non-coding RNA Lipid-Droplet Transporter (LIPTER).

The concentration of free fatty acids (FA) (f, n = 5 mice each group) were quantified using the Free Fatty Acid Assay kit (ab65341), and triglyceride (TG) concentrations (g, n = 7 mice each group) were quantified using the Triglyceride Quantification Assay Kit (ab65336).

Approximately 25 mg of mouse heart tissue per heart was collected and homogenized in 500 μl lipid extraction buffer (ab211044) using a Beadbug Homogenizer. After centrifugation, the supernatant was removed to a new tube. FA or TAG concentration was measured according to the manufacturer’s protocols. The FA and TAG concentrations in WT and genetically modified mouse hearts were normalized to the collected heart tissue weights.

• FuncS

Supplier Data

Functional Studies - Free Fatty Acid Assay Kit - Quantification (AB65341)

Colorimetric standard curve : mean of duplicates (+/-SD) with background readings subtracted.

• FuncS

Lab

Functional Studies - Free Fatty Acid Assay Kit - Quantification (AB65341)

Flourometric standard curve : mean of duplicates (+/-SD) with background readings subtracted.

• FuncS

Lab

Functional Studies - Free Fatty Acid Assay Kit - Quantification (AB65341)

Free Fatty Acid measured in biologicals showing concentration (μM).

• FuncS

PubMed

Functional Studies - Free Fatty Acid Assay Kit - Quantification (AB65341)

Plasma FFA levels were measured using ab65341 in male and female wild-type (WT) or AT₂KO mice either on normal diet (ND) or high fat diet (HFD).

Samuel P et al., PLoS One 8(1), Fig 7a. DOI: 10.1371/journal.pone.0048425. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

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Functional Studies - Free Fatty Acid Assay Kit - Quantification (AB65341)

Lipolysis were evaluated by measuring free fatty acids released into the culture medium of siCreg1 or si-Scramble 3T3-L1 cells using ab65341, which were treated with BMS-345541 (5 μmol/L), an NF-κB pathway inhibitor, or vehicle (DMSO) for 48 hours prior to lipolysis assessment. ns, no significance; and ***P<0.001.

Tian, Xiaoxiang et al., PloS one vol. 12,5 e0176873., Fig 6, doi:10.1371/journal.pone.0176873

• Biochemical assay

PubMed

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

Triglyceride assay kit ab65336 and free fatty acid assay kit ab65341 used with liver tissue and plasma samples respectively.

Samuel et al. used the triglyceride assay kit and free fatty acid assay kit to understand gender differences in metabolic syndrome disorders in angiotensin receptor AT2R knockout mice.

Plasma free fatty acid levels were measured by colorimetric method using the Abcam free fatty acid quantification kit.

Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD). For hepatic triglyceride measurement, livers were dissected and snap-frozen in liquid nitrogen and stored at −80°C. In brief, lipids were extracted by homogenizing 25 mg liver tissue in 1 ml 5% Triton-X100 in water, then slowly heated to 80°C in water bath for 5 min. The samples were cooled down and again heated to solubilize all triglycerides into solution. The samples were centrifuged for 5 min and supernatants were diluted 10 fold with dH2O for quantification.

• Biochemical assay

PubMed

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

Triglyceride assay kit ab65336 and Free Fatty Acid assay kit ab65341 used with mouse liver tissue.

Yue et al. used triglyceride assay kit ab65336 and free fatty acid assay kit ab65341 to investigate the protective effects of IL-22 on alleviating alcoholic hepatitis.

Administration of IL-22 reduces alcohol-induced lipid accumulation in mouse liver. PF (pair fed), AF (alcohol fed), AF+IL-22 (alcohol fed with IL-22 treatment).

(A) BODIPY 493/503 staining of mouse liver. Neutral lipids were stained in green, and nuclei were counterstained in blue. (B) Hepatic triglyceride (TG) and free fatty acid (FFA) levels.

Quantification assays of triglycerides (TG) and free fatty acids (FFA) in the liver were conducted using commercial kits from Abcam (Waltham, MA; ab65336 and ab65341) per the manufacturer’s instructions.

• Biochemical assay

PubMed

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

Triglyceride assay kit ab65336, Cholesterol assay kit ab65390, and Free Fatty Acid assay kit ab65341 used with rat serum and rat liver.

Li et al. used Triglyceride assay kit ab65336, Cholesterol assay kit ab65359, and Free Fatty Acid assay kit ab65341 to investigate the impact of using L. reuteri treatment to ameliorate the impact of biorhythm disorder-ignited dyslipidemia in long-term darkness treated rats.

The concentrations of triglycerides (TG), total cholesterol (CHOL), HDL-cholesterol (HDL-C), and free fatty acids (NEFA) in rat sera and liver tissues were detected using Triglyceride Quantification Assay Kit (ab65336), HDL and LDL/VLDL Cholesterol Assay Kit (ab65390). and Free Fatty Acid Quantification Assay Kit (ab65341), respectively. All procedures were carried out following the recommended instructions provided by the kit manufacturers.

• Other

Supplier Data

Other - Free Fatty Acid Assay Kit - Quantification (AB65341)

Representative image of Free Fatty Acid Assay Kit - Quantification ab65341

Components shown from left to right :

- OxiRed Probe

- ACS Reagent

- Palmitic Acid Standard

- Acyl CoA Enzyme Mix

- Enhancer I

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.