AB176768

Free Fatty Acid Uptake Assay Kit (Fluorometric)

Name: Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768) | Abcam
Brand: Abcam Limited
SKU: AB176768
Price: 555 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(28 Publications)

Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768) provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters.

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Functional Studies - Free Fatty Acid Uptake Assay Kit (Fluorometric) (AB176768)

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Functional Studies - Free Fatty Acid Uptake Assay Kit (Fluorometric) (AB176768)

Excitation and Emission Spectra of Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768)

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Functional Studies - Free Fatty Acid Uptake Assay Kit (Fluorometric) (AB176768)

Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.

Cells were plated at 50,000 cells/100μL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37 °C, 5% CO₂ incubator for 30 min. At the end of the incubation time, 100 μL of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a plate reader using bottom read mode. A – fibroblasts (Control); B – fibroblasts (Insulin); C – adipocytes (Control); D– adipocytes (Insulin).

Key facts

Detection method

Fluorescent

Sample types

Adherent cells

Assay type

Cell-based (quantitative)

Assay time

Assay Platform

Microplate reader

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Product details

Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768) provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. The kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate. This fatty acid uptake assay kit can be performed on any fluorescence microplate reader with a bottom-read mode at Ex/Em = 485/515 nm or FITC channel. The assay can be performed in 96-well or 384-well microtiter plates in a simple mix-and-read procedure, and easily adapted for high throughput screening applications.

Fatty acid uptake is an important therapeutic target for the treatment of many human diseases such as obesity, type 2 diabetes and hepatic steatosis.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab176768-100Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Assay Buffer", "number":"AB176768-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"DMSO", "number":"AB176768-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"TF2-C12 Fatty Acid", "number":"AB176768-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

See full target information Free Fatty Acid

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4743 PubMed40399268

2025

Exercise-induced CLCF1 attenuates age-related muscle and bone decline in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Sook Kang,Jung Ha Kim,Min Ju Kim,Byungkuk Min,Seung-Min Lee,Ga-Yeon Go,Ji-Won Kim,Seongwan Kim,Ju Yeon Kwak,Sung-Wook Chun,Wook Song,Hyo Youl Moon,Sun Gun Chung,Dong-Ho Park,Ji Hoon Park,Chuna Kim,Kwang-Pyo Lee,Eun-Soo Kwon,Nacksung Kim,Ki-Sun Kwon,Yong Ryoul Yang

Experimental & molecular medicine 57:349-363 PubMed39900792

2025

Golgi condensation causes intestinal lipid accumulation through HIF-1α-mediated GM130 ubiquitination by NEDD4.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunsoo Kim,Channy Park,Xiaofan Wei,Arun Chhetri,Laxman Manandhar,Gyuho Jang,Jaetaek Hwang,Batchingis Chinbold,Chagtsalmaa Chuluunbaatar,Hyug Moo Kwon,Raekil Park

International journal of molecular sciences 25: PubMed38791126

2024

Upregulation of Hepatic Glutathione S-Transferase Alpha 1 Ameliorates Metabolic Dysfunction-Associated Steatosis by Degrading Fatty Acid Binding Protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Jiang,Hu Li,Mei Tang,Lei Lei,Hong-Ying Li,Biao Dong,Jian-Rui Li,Xue-Kai Wang,Han Sun,Jia-Yu Li,Jing-Chen Xu,Yue Gong,Jian-Dong Jiang,Zong-Gen Peng

Nature communications 15:2869 PubMed38693144

2024

A patient-based iPSC-derived hepatocyte model of alcohol-associated cirrhosis reveals bioenergetic insights into disease pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bani Mukhopadhyay,Cheryl Marietta,Pei-Hong Shen,Abdul Oiseni,Faridoddin Mirshahi,Maria Mazzu,Colin Hodgkinson,Eli Winkler,Qiaoping Yuan,Daniel Miranda,George Kunos,Arun J Sanyal,David Goldman

Translational oncology 45:101941 PubMed38692197

2024

MAPKAP1 orchestrates macrophage polarization and lipid metabolism in fatty liver-enhanced colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Huang,Zhenqiu Yu,Dejun Cui,Fawang Du

Cell reports. Medicine 4:101234 PubMed37852179

2023

Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Tang,Jin Xu,Wei Wang,Qingcai Meng,Chenghao Shao,Yiyin Zhang,Yubin Lei,Zifeng Zhang,Yuan Liu,Qiong Du,Xiangjie Sun,Di Wu,Chen Liang,Jie Hua,Bo Zhang,Xianjun Yu,Si Shi

Journal of lipid research 63:100221 PubMed35526561

2022

Neuronal growth regulator 1 promotes adipocyte lipid trafficking via interaction with CD36.

Applications

Unspecified application

Species

Unspecified reactive species

Ara Yoo,Yeonhee Joo,Yeongmi Cheon,Sung Joong Lee,Soojin Lee

British journal of pharmacology 179:1411-1432 PubMed34783017

2022

A novel HSF1 activator ameliorates non-alcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Rao,Chan Li,Yu-Tao Hu,Yao-Hao Xu,Bing-Bing Song,Shi-Yao Guo,Zhi Jiang,Dan-Dan Zhao,Shuo-Bin Chen,Jia-Heng Tan,Shi-Liang Huang,Qing-Jiang Li,Xiao-Jun Wang,Ying-Jun Zhang,Ji-Ming Ye,Zhi-Shu Huang

Cell death & disease 12:1108 PubMed34839347

2021

SLC25A1 promotes tumor growth and survival by reprogramming energy metabolism in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yang,Jiaxing He,Bo Zhang,Zhansheng Zhang,Guozhan Jia,Shiqi Liu,Tao Wu,Xianli He,Nan Wang

Journal for immunotherapy of cancer 9: PubMed34145030

2021

S100A4 enhances protumor macrophage polarization by control of PPAR-γ-dependent induction of fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangqing Liu,Huilei Zhang,Yanan Li,Yana Zhang,Yangyang Bian,Yanqiong Zeng,Xiaohan Yao,Jiajia Wan,Xu Chen,Jianru Li,Zhaoqing Wang,Zhihai Qin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Free Fatty Acid Uptake Assay Kit (Fluorometric)

Functional Studies - Free Fatty Acid Uptake Assay Kit (Fluorometric) (AB176768)

Functional Studies - Free Fatty Acid Uptake Assay Kit (Fluorometric) (AB176768)

Key facts

Detection method

Sample types

Assay type

Assay time

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (28)

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