Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.
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- Nature communications 14:66442023Glycerol-weighted chemical exchange saturation transfer nanoprobes allow FH dual-modality magnetic resonance imaging-guided cancer radiotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong A,Haoyu Wang,Chaoqun Nie,Zhaoguo Han,Meifang Zhou,Olagbaju Oluwatosin Atinuke,Kaiqi Wang,Xiance Wang,Shuang Liu,Jingshi Zhao,Wenju Qiao,Xiaohong Sun,Lina Wu,Xilin SunPubMed 37863898
- Nature 621:138-1452023Xiphoid nucleus of the midline thalamus controls cold-induced food seeking.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeeraj K Lal,Phuong Le,Samarth Aggarwal,Alan Zhang,Kristina Wang,Tianbo Qi,Zhengyuan Pang,Dong Yang,Victoria Nudell,Gene W Yeo,Alexander S Banks,Li YePubMed 37587337
- Antioxidants (Basel, Switzerland) 12:2023Effect of Crocetin on Basal Lipolysis in 3T3-L1 Adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco J Cimas,Miguel Ángel De la Cruz-Morcillo,Carmen Cifuentes,Natalia Moratalla-López,Gonzalo L Alonso,Eduardo Nava,Sílvia LlorensPubMed 37371984
- Human molecular genetics 32:2219-22282023Breast cancer cell-derived exosome-delivered microRNA-155 targets UBQLN1 in adipocytes and facilitates cancer cachexia-related fat loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Sun,Z Wang,F Yao,K Sun,Z Li,S Sun,C LiPubMed 37017334
- Metabolites 13:2023Luteolin-7-O-rutinoside Protects RIN-5F Cells from High-Glucose-Induced Toxicity, Improves Glucose Homeostasis in L6 Myotubes, and Prevents Onset of Type 2 Diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPandurangan Subash-Babu,Sahar Abdulaziz AlSedairy,Manal Abdulaziz Binobead,Ali A AlshatwiPubMed 36837888
- Cells 11:2022Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Wang,Lieke E J van Iersel,Charlotte E Pelgrim,Jingyi Lu,Ingrid van Ark,Thea Leusink-Muis,Harry R Gosker,Ramon C J Langen,Annemie M W J Schols,Josep M Argilés,Ardy van Helvoort,Aletta D Kraneveld,Johan Garssen,Paul A J Henricks,Gert Folkerts,Saskia BraberPubMed 36139468
- Adipocyte 11:487-5002022Cancer cell-derived exosomal miR-425-3p induces white adipocyte atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnwen Liu,Wenxia Pan,Shutong Zhuang,Yuanzhi Tang,Haitao ZhangPubMed 35941833
- Scientific reports 12:66122022Capsaicin directly promotes adipocyte browning in the chemical compound-induced brown adipocytes converted from human dermal fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukimasa Takeda,Ping DaiPubMed 35459786
- Frontiers in pharmacology 13:8528582022P2X7 Activation Enhances Lipid Accumulation During Adipocytes Differentiation Through Suppressing the Expression of Sirtuin-3, Sirtuin-5, and Browning Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChien-Hsieh Chiang,Ching-Yuan Cheng,Yi-Ting Lien,Kuo-Chin Huang,Wan-Wan LinPubMed 35462937
- Physiological reports 10:e151462022Glycerol contained in vaping liquids affects the liver and aspects of energy homeostasis in a sex-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAriane Lechasseur,Mathilde Mouchiroud,Félix Tremblay,Gabrielle Bouffard,Nadia Milad,Marie Pineault,Michaël Maranda-Robitaille,Joanie Routhier,Marie-Josée Beaulieu,Sophie Aubin,Mathieu Laplante,Mathieu C MorissettePubMed 35075822
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
1 - 10000 µM
- Assay time
40m
- Sensitivity
> 1 µM
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Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
1 - 10000 µM
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 1 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.
In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 μM range) of glycerol.
Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K630 Free Glycerol Colorimetric/Fluorometric Assay Kit. K630-100 is the same size as the 100 test size of ab65337.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glycerol also known as glycerol anhydrous or glycérol is a simple polyol compound with a molecular weight of approximately 92.09 g/mol. Glycerol is expressed widely in various tissues and plays a considerable role as a backbone in the structure of triglycerides and phospholipids thereby impacting lipid biology significantly. It acts mechanically in lipid metabolism by serving as a substrate for glycerolipid synthesis and provides energy through its conversion to glucose or dihydroxyacetone phosphate.
- Biological function summary
Glycerol metabolism is significant in energy storage and lipid regulation. It does not function in isolation but is part of various lipid complexes that regulate lipid homeostasis. Through its involvement it aids in the formation of essential lipid components that are important for membrane structure and function. The ability of cells to store energy efficiently is largely dependent on glycerol's capability to integrate into and support these lipid structures.
- Pathways
Glycerol participates in glycerolipid metabolism and gluconeogenesis. The glycolytic pathway is especially important as glycerol serves as a potential precursor for glucose production connecting it to energy supply chains within the organism. Proteins like glycerol kinase and glycerol-3-phosphate dehydrogenase play critical roles in these conversions and metabolic processes underlying how glycerol fits into broader biological functions.
- Associated diseases and disorders
Glycerol has connections to metabolic conditions such as glycerol kinase deficiency and nonalcoholic fatty liver disease. Its metabolism's rate and efficiency can influence lipid accumulation and related metabolic syndromes. The enzyme glycerol kinase which phosphorylates glycerol to form glycerol-3-phosphate associates directly with glycerol's relevance in these diseases. Understanding these connections may lead to insights into managing or alleviating related metabolic disease symptoms.
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5 product images
Functional Studies - Free Glycerol Assay Kit (ab65337)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Free Glycerol Assay Kit (ab65337)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Free Glycerol Assay Kit (ab65337)
Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.
Functional Studies - Free Glycerol Assay Kit (ab65337)
Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.
Functional Studies - Free Glycerol Assay Kit (ab65337)
Free Glycerol Assay Kit - Functional Studies.
a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).
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