AB65337

Free Glycerol Assay Kit

Name: Free Glycerol Assay Kit. Colorimetric/Fluorometric. (ab65337) | Abcam
Brand: Abcam Limited
SKU: AB65337
Price: 785 USD
Availability: InStock

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(45 Publications)

Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to quantify free glycerol concentration. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.

- Cited in over 35 publications

6 Images

Functional Studies - Free Glycerol Assay Kit (AB65337)

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Lab

Functional Studies - Free Glycerol Assay Kit (AB65337)

Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.

FuncS

Supplier Data

Functional Studies - Free Glycerol Assay Kit (AB65337)

Free Glycerol Assay Kit - Functional Studies.

a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).

FuncS

Lab

Functional Studies - Free Glycerol Assay Kit (AB65337)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

FuncS

Lab

Functional Studies - Free Glycerol Assay Kit (AB65337)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

FuncS

Lab

Functional Studies - Free Glycerol Assay Kit (AB65337)

Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.

Other - Free Glycerol Assay Kit (AB65337)

Other

Supplier Data

Other - Free Glycerol Assay Kit (AB65337)

Representative image of Free Glycerol Assay Kit ab65337

Components shown from left to right :

- OxiRed Probe

- Glycerol Standard

- Enzyme Mix VI

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Results type

Quantitative

Sensitivity

> 1 µM

Range

1 - 10000 µM

Assay time

40m

Assay Platform

Microplate reader

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Product details

Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.

In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).

This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 μM range) of glycerol.

Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K630 Free Glycerol Colorimetric/Fluorometric Assay Kit. K630-100 is the same size as the 100 test size of ab65337.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65337-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65337-CMP03", "productcode":"AB309437" }, { "size":"1 x 200 µL", "name":"Glycerol Standard", "number":"AB65337-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enzyme Mix VI", "number":"AB65337-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB65337-CMP01", "productcode":"AB323940" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol also known as glycerol anhydrous or glycérol is a simple polyol compound with a molecular weight of approximately 92.09 g/mol. Glycerol is expressed widely in various tissues and plays a considerable role as a backbone in the structure of triglycerides and phospholipids thereby impacting lipid biology significantly. It acts mechanically in lipid metabolism by serving as a substrate for glycerolipid synthesis and provides energy through its conversion to glucose or dihydroxyacetone phosphate.

Biological function summary

Glycerol metabolism is significant in energy storage and lipid regulation. It does not function in isolation but is part of various lipid complexes that regulate lipid homeostasis. Through its involvement it aids in the formation of essential lipid components that are important for membrane structure and function. The ability of cells to store energy efficiently is largely dependent on glycerol's capability to integrate into and support these lipid structures.

Pathways

Glycerol participates in glycerolipid metabolism and gluconeogenesis. The glycolytic pathway is especially important as glycerol serves as a potential precursor for glucose production connecting it to energy supply chains within the organism. Proteins like glycerol kinase and glycerol-3-phosphate dehydrogenase play critical roles in these conversions and metabolic processes underlying how glycerol fits into broader biological functions.

Glycerol has connections to metabolic conditions such as glycerol kinase deficiency and nonalcoholic fatty liver disease. Its metabolism's rate and efficiency can influence lipid accumulation and related metabolic syndromes. The enzyme glycerol kinase which phosphorylates glycerol to form glycerol-3-phosphate associates directly with glycerol's relevance in these diseases. Understanding these connections may lead to insights into managing or alleviating related metabolic disease symptoms.

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Product protocols

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Target data

See full target information Glycerol

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:200 PubMed40571693

2025

D-lactate and glycerol as potential biomarkers of sorafenib activity in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Pedretti,Francesca Palermo,Miriana Braghin,Gabriele Imperato,Pasquale Tomaiuolo,Meral Celikag,Marta Boccazzi,Veronica Vallelonga,Lorenzo Da Dalt,Giuseppe Danilo Norata,Giorgia Marisi,Ilario Giovanni Rapposelli,Andrea Casadei-Gardini,Serena Ghisletti,Maurizio Crestani,Emma De Fabiani,Nico Mitro

Nature 643:192-200 PubMed39695227

2024

Lithocholic acid phenocopies anti-ageing effects of calorie restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Qu,Yan Chen,Yu Wang,Shating Long,Weiche Wang,Heng-Ye Yang,Mengqi Li,Xiao Tian,Xiaoyan Wei,Yan-Hui Liu,Shengrong Xu,Cixiong Zhang,Mingxia Zhu,Sin Man Lam,Jianfeng Wu,Chuyu Yun,Junjie Chen,Shengye Xue,Baoding Zhang,Zhong-Zheng Zheng,Hai-Long Piao,Changtao Jiang,Hao Guo,Guanghou Shui,Xianming Deng,Chen-Song Zhang,Sheng-Cai Lin

Nutrients 16: PubMed39339774

2024

Royal Jelly Exerts a Potent Anti-Obesity Effect in Rats by Activating Lipolysis and Suppressing Adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa Hasanain Felemban,Ghedeir M Alshammari,Abu ElGasim Ahmed Yagoub,Ali Saleh,Mohammed Abdo Yahya

Antioxidants (Basel, Switzerland) 13: PubMed39334757

2024

L. (Safflower) Flower Extract Attenuates Hepatic Injury and Steatosis in a Rat Model of Type 2 Diabetes Mellitus via Nrf2-Dependent Hypoglycemic, Antioxidant, and Hypolipidemic Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Nuha Saad Alshareef,Sahar Abdulaziz AlSedairy,Laila Naif Al-Harbi,Ghedeir M Alshammari,Mohammed Abdo Yahya

Nature communications 15:6768 PubMed39117652

2024

Light-responsive adipose-hypothalamus axis controls metabolic regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Tadataka Tsuji,Vladimir Tolstikov,Yang Zhang,Tian Lian Huang,Henrique Camara,Meghan Halpin,Niven R Narain,King-Wai Yau,Matthew D Lynes,Michael A Kiebish,Yu-Hua Tseng

Scientific reports 14:18252 PubMed39107469

2024

Functional roles of pantothenic acid, riboflavin, thiamine, and choline in adipocyte browning in chemically induced human brown adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yukimasa Takeda,Ping Dai

Scientific reports 14:10789 PubMed38734719

2024

Angiotensin II participates in mitochondrial thermogenic functions via the activation of glycolysis in chemically induced human brown adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yukimasa Takeda,Toshikazu Yoshikawa,Ping Dai

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400426 PubMed38666466

2024

Adaptive Metabolic Responses Facilitate Blood-Brain Barrier Repair in Ischemic Stroke via BHB-Mediated Epigenetic Modification of ZO-1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Li,Yilin Liu,Jihao Wu,Xiong Chen,Qiying Lu,Kai Xia,Congyuan Liu,Xin Sui,Yixuan Liu,Yiling Wang,Yuan Qiu,Jinsi Chen,Yi Wang,Ruijun Li,Yucheng Ba,Jiayun Fang,Weijun Huang,Zhengqi Lu,Yanbing Li,Xinxue Liao,Andy Peng Xiang,Yinong Huang

International journal of molecular sciences 25: PubMed38473981

2024

Skin Anti-Aging Potential through Whitening and Wrinkle Improvement Using Fermented Oil Derived from Larvae.

Applications

Unspecified application

Species

Unspecified reactive species

Dooseon Hwang,Tae-Won Goo,Seung Hun Lee,Eun-Young Yun

Obesity (Silver Spring, Md.) 32:352-362 PubMed38018497

2023

Follistatin-like 1 is a myokine regulating lipid mobilization during endurance exercise and recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Sun Nam,Su-Jeong Park,Chul Woo Ahn,Eun-Suk Cho,Hee-Joo Kim,YuSik Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Free Glycerol Assay Kit

Functional Studies - Free Glycerol Assay Kit (AB65337)

Functional Studies - Free Glycerol Assay Kit (AB65337)

Functional Studies - Free Glycerol Assay Kit (AB65337)

Functional Studies - Free Glycerol Assay Kit (AB65337)

Functional Studies - Free Glycerol Assay Kit (AB65337)

Other - Free Glycerol Assay Kit (AB65337)

Key facts

Detection method

Sample types

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (45)

Product promise