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AB155899

Free Glycerol Assay Kit II (Colorimetric)

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(1 Publication)

Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays.
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Functional Studies - Free Glycerol Assay Kit II (Colorimetric) (AB155899)
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Supplier Data

Functional Studies - Free Glycerol Assay Kit II (Colorimetric) (AB155899)

Glycerol Standard Curve [a]. Measurement of free glycerol in bovine liver (50 µg), Jurkat cells (40 µg), HeLa cells (30 µg), fish egg (58 µg) & human serum (2 µl) [b]. Assays were performed following kit protocol.

Other - Free Glycerol Assay Kit II (Colorimetric) (AB155899)
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Supplier Data

Other - Free Glycerol Assay Kit II (Colorimetric) (AB155899)

Representative image of Free Glycerol Assay Kit II (Colorimetric) ab155899

Components shown from left to right :

- Glycerol Standard

- Developer Solution III

- Glycerol Enzymatic Developer

- Glycerol Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue, Suspension cells, Tissue Homogenate, Cell culture supernatant, Serum, Adherent cells

Results type

Quantitative

Sensitivity

< 20 µM

Range

2 - 10 nmol/well

Assay time

1h 30m

Assay Platform

Microplate reader

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Product details

Abcam's Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, Glycerol in the presence of Glycerol Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. Free Glycerol Assay Kit II is simple, rapid and high-throughput adaptable. This assay kit can detect less than 20 μM of free Glycerol in various biological samples.

Detection method: colorimetric – based on enzyme activity

This product is manufactured by BioVision, an Abcam company and was previously called K634 Free Glycerol Colorimetric Assay Kit II. K634-100 is the same size as the 100 test size of ab155899.

Glycerol is a central component of most lipids. It acts as a backbone for triglycerides and phospholipids, which play an important role in metabolism and cell membrane structure. Due to its low toxicity, glycerol is widely used in the pharmaceutical, food and cosmetic industries.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab155899-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Glycerol Enzymatic Developer", "number":"AB155899-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Glycerol Assay Buffer", "number":"AB155899-CMP01", "productcode":"" }, { "size":"1 x 1 Each", "name":"Developer Solution III", "number":"AB155899-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"Glycerol Standard", "number":"AB155899-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol also known as glycerol anhydrous or glycérol is a simple polyol compound with a molecular weight of approximately 92.09 g/mol. Glycerol is expressed widely in various tissues and plays a considerable role as a backbone in the structure of triglycerides and phospholipids thereby impacting lipid biology significantly. It acts mechanically in lipid metabolism by serving as a substrate for glycerolipid synthesis and provides energy through its conversion to glucose or dihydroxyacetone phosphate.
Biological function summary

Glycerol metabolism is significant in energy storage and lipid regulation. It does not function in isolation but is part of various lipid complexes that regulate lipid homeostasis. Through its involvement it aids in the formation of essential lipid components that are important for membrane structure and function. The ability of cells to store energy efficiently is largely dependent on glycerol's capability to integrate into and support these lipid structures.

Pathways

Glycerol participates in glycerolipid metabolism and gluconeogenesis. The glycolytic pathway is especially important as glycerol serves as a potential precursor for glucose production connecting it to energy supply chains within the organism. Proteins like glycerol kinase and glycerol-3-phosphate dehydrogenase play critical roles in these conversions and metabolic processes underlying how glycerol fits into broader biological functions.

Glycerol has connections to metabolic conditions such as glycerol kinase deficiency and nonalcoholic fatty liver disease. Its metabolism's rate and efficiency can influence lipid accumulation and related metabolic syndromes. The enzyme glycerol kinase which phosphorylates glycerol to form glycerol-3-phosphate associates directly with glycerol's relevance in these diseases. Understanding these connections may lead to insights into managing or alleviating related metabolic disease symptoms.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2018:6053727 PubMed30533173

2018

Geniposide Protects against Obesity-Related Cardiac Injury through AMPK- and Sirt1-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Guo Ma,Chun-Yan Kong,Peng Song,Xin Zhang,Yu-Pei Yuan,Qi-Zhu Tang
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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