JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB273329

Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(2 Publications)

Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (ab273329) provides a quick, reliable test that allows the measurement of Fructose-1,6-Bisphosphatase (FBP) enzymatic activity in various samples.

View Alternative Names

FBP, FBP1, FBPase 1, Liver FBPase

4 Images
Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)
  • FuncS

Supplier Data

Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)

Fructose-1,6-Bisphosphatase Activity assay pathway

Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)
  • FuncS

Supplier Data

Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)

Example data.

Kinetic measurement of Fructose-1,6-Biosphophatase activity from various samples.

Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)
  • FuncS

Supplier Data

Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)

Example data.

Specific FBP Activity was calculated in lysates prepared from rat liver (3.2μg protein), rat kidney (8μg protein) and HeLa lysate (4μg protein).

Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)
  • FuncS

Supplier Data

Functional Studies - Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (AB273329)

Example data.

F6P standard curve.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity (quantitative)

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (ab273329) provides a quick, reliable test that allows the measurement of Fructose-1,6-Bisphosphatase (FBP) enzymatic activity in various samples. In this assay, FBP hydrolyzes Fructose-1,6-Bisphosphate into Fructose-6-Phosphate (F6P). F6P is used in an enzyme coupled reaction which reduces a chromophore forming a product with a stable signal that can measured at OD = 450 nm.

The assay is simple, sensitive and can detect lower than 100 μU of FBP in variety of samples.

This product is manufactured by BioVision, an Abcam company and was previously called K590 Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric). K590-100 is the same size as the 100 test size of ab273329.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273329-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"NADPH Substrate Mix", "number":"AB273329-CMP06" }, { "size":"1 x 1 Vial", "name":"FBP Substrate", "number":"AB273329-CMP07" }, { "size":"1 x 1 Vial", "name":"FBP Positive Control", "number":"AB273329-CMP05" }, { "size":"1 x 1 Vial", "name":"F6P Standard", "number":"AB273329-CMP01" }, { "size":"1 x 1 Vial", "name":"Developer Mix P", "number":"AB273329-CMP04" }, { "size":"1 x 1 Vial", "name":"Converter Mix G", "number":"AB273329-CMP03" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB273329-CMP02" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273329-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"NADPH Substrate Mix", "number":"AB273329-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"FBP Substrate", "number":"AB273329-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"FBP Positive Control", "number":"AB273329-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"F6P Standard", "number":"AB273329-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix P", "number":"AB273329-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix G", "number":"AB273329-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB273329-CMP02", "productcode":"AB323940" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fructose-16-bisphosphatase (FBP1) and fructose-16-bisphosphatase 2 (FBP2) are enzymes that play an important role in gluconeogenesis. They catalyze the conversion of fructose-16-bisphosphate to fructose-6-phosphate an important metabolic step in glucose production. FBP1 sometimes called liver FBPase is a protein of approximately 37 kDa and it is mainly expressed in the liver kidneys and adipose tissue. FBP2 also known as muscle FBPase is primarily found in muscle tissues. These enzymes share similar enzymatic functions but differ in their regulation and tissue distribution.
Biological function summary

These enzymes help regulate glucose levels in the body. They function as part of a larger enzyme complex involved in energy homeostasis. FBP1 plays a significant role in balancing blood sugar levels during fasting by promoting gluconeogenesis in the liver. FBP2 on the other hand contributes to muscle energy management. The activity of these enzymes is tightly regulated by allosteric inhibitors and activators and they interact with other metabolic enzymes to maintain homeostasis.

Pathways

Fructose-16-bisphosphatase is involved in the gluconeogenesis pathway and is closely linked with the glycolysis pathway. In gluconeogenesis FBP1 and FBP2 convert fructose-16-bisphosphate to fructose-6-phosphate with dihydroxyacetone phosphate as an important relay. They work in tandem with other enzymes like phosphoenolpyruvate carboxykinase to facilitate glucose production. This makes them integral to metabolic pathways that provide glucose during periods of low carbohydrate intake.

Dysregulation of FBP1 and FBP2 contributes to metabolic disorders such as type 2 diabetes and hypoglycemia. Overactivity of FBP1 can lead to increased glucose production complicating glucose management in diabetes mellitus. On the other hand FBP2 mutations may impair muscle function and energy utilization. The proteins' imbalances also relate to insulin resistance as their activity influences insulin signaling pathways within metabolic disorders.
style
left-column

Product protocols

style
left-column

Target data

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.
See full target information FBP1

Additional targets

FBP2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 35:1009-1021.e9 PubMed37084733

2023

Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gu,Yahui Zhu,Kosuke Watari,Maiya Lee,Junlai Liu,Sofia Perez,Melinda Thai,Joshua E Mayfield,Bichen Zhang,Karina Cunha E Rocha,Fuming Li,Laura C Kim,Alexander C Jones,Igor H Wierzbicki,Xiao Liu,Alexandra C Newton,Tatiana Kisseleva,Jun Hee Lee,Wei Ying,David J Gonzalez,Alan R Saltiel,M Celeste Simon,Michael Karin

International journal of molecular sciences 24: PubMed36613872

2022

Protein Kinase CK2 Contributes to Glucose Homeostasis by Targeting Fructose-1,6-Bisphosphatase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Mandy Pack,Tim Nikolai Gulde,Michelle Victoria Völcker,Anne S Boewe,Selina Wrublewsky,Emmanuel Ampofo,Mathias Montenarh,Claudia Götz
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB284537

Fructose-1,6-Bisphosphate Assay Kit (Fluorometic)

Enzyme activity assay - Fructose-1,6-Bisphosphate Assay Kit (Fluorometic) (AB284537)

  • 0
  • 0
Assay Kits

AB196994

Aldolase Activity Assay Kit (Colorimetric)

Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)

  • 0
  • 0
Assay Kits

AB155898

Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric)

Functional Studies - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (AB155898)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com