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AB284537

Fructose-1,6-Bisphosphate Assay Kit (Fluorometic)

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(1 Publication)

Fructose-1,6-Bisphosphate Assay Kit (ab284537) provides a quick and easy way to measure Fructose-1,6-Bisphosphate (F1,6BP) in a variety of sample types.
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Enzyme activity assay - Fructose-1,6-Bisphosphate Assay Kit (Fluorometic) (AB284537)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Fructose-1,6-Bisphosphate Assay Kit (Fluorometic) (AB284537)

Fructose-1,6-Bisphosphate assay pathway

Enzyme activity assay - Fructose-1,6-Bisphosphate Assay Kit (Fluorometic) (AB284537)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Fructose-1,6-Bisphosphate Assay Kit (Fluorometic) (AB284537)

PicoProbe­™ Fructose-1,6-Bisphosphate Assay Kit (Fluorometic).

Measurement of Fructose-1,6-Bisphosphate in mouse liver lysate (50 μg protein) and germinated barley seed lysate (G7) (10 μg protein). Assays were performed following the protocol.

Enzyme activity assay - Fructose-1,6-Bisphosphate Assay Kit (Fluorometic) (AB284537)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Fructose-1,6-Bisphosphate Assay Kit (Fluorometic) (AB284537)

PicoProbe­™ Fructose-1,6-Bisphosphate Assay Kit (Fluorometic).

Fructose-1,6-Bisphosphate Standard Curve generated using this kit.

Key facts

Sample types

Cell Lysate, Tissue, Tissue Lysate

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Reactivity data

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Product details

This Fructose-1,6-Bisphosphate Assay Kit (ab284537) provides a quick and easy way to measure Fructose-1,6-Bisphosphate (F1,6BP) in a variety of sample types. In the assay, F1,6BP is hydrolyzed by Fructose-1,6-Bisphosphatase (FBP) into inorganic phosphate and F6P. F6P then through several intermediate reactions reacts with the PicoProbe to generate a stable fluorophore measured at Ex/Em = 535/587 nm.

The assay is simple, easy to perform, sensitive and can detect F1,6BP less than 0.5 uM in a variety of samples.

Other Notes
This product was previously called K2036 Biovision PicoProbe Fructose-1,6-Bisphosphate Assay Kit (F). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fructose-16-bisphosphate often referred to as F 1 6 BP or fructose bisphosphate plays a critical role in glycolysis. It exists as a potent allosteric activator of pyruvate kinase which is essential for the conversion of phosphoenolpyruvate to pyruvate during energy production. The compound has a molecular mass of approximately 360.12 g/mol and is expressed in various tissues particularly those with high metabolic rates such as the liver muscle and brain. Fructose-16-bisphosphate is not a protein but an intermediate substrate but it is tightly regulated and interacts with several enzymes during glycolysis.
Biological function summary

Fructose 16 bisphosphate serves as a pivotal intermediate in the glycolytic pathway. It originates from the phosphorylation of fructose 6-phosphate by the enzyme phosphofructokinase-1. The compound's high energy content facilitates its role in the metabolic cycle which directly influences the ATP yield in cells. Fructose 1 6 bisphosphate also acts as a signaling molecule connecting to various metabolic processes and is part of the pyruvate kinase enzyme complex. This relationship highlights its significance in sustaining cellular energy requirements.

Pathways

Fructose 16 bisphosphate plays a fundamental role in glycolysis and gluconeogenesis. In glycolysis its formation represents a commitment step catalyzed by phosphofructokinase-1 distinctly connecting it to enzymes such as aldolase which splits it into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. In gluconeogenesis fructose 16 bisphosphate is converted back into fructose 6-phosphate a process catalyzed by fructose 16-bisphosphatase. This conversion highlights its dual function in energy generation and glucose synthesis linking fructose 1 6 BP to many metabolic cycle proteins and processes.

Fructose 1 6 bisphosphate is associated with metabolic conditions such as diabetes mellitus and fructose-16-bisphosphatase deficiency. In diabetes mellitus the inefficient regulation of glycolysis and gluconeogenesis partly due to fructose bisphosphate and related enzymes can lead to impaired glucose homeostasis. Fructose-16-bisphosphatase deficiency a metabolic disorder directly relates to the accumulation of the fructose 16 bisphosphate causing disruption in gluconeogenesis and subsequent hypoglycemia. These connections highlight the compound's relevance in metabolism and disease emphasizing the need for further research into its role alongside associated proteins.
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Product protocols

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Target data

See full target information beta-D-fructofuranose 1,6-bisphosphate(4-)
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107147 PubMed38460940

2024

Restricted glycolysis is a primary cause of the reduced growth rate of zinc-deficient yeast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Colin W MacDiarmid,Janet Taggart,Michael Kubisiak,David J Eide
View all publications
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websiteProtocolBooklet
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