Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples.
Images
Publications
Filter by
- Cell reports 41:1115982022MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Jung Yoo,Dong Wook Choi,Yeon Jin Roh,Yoon-Mi Lee,Ji-Hong Lim,Soohak Eo,Ho-Jae Lee,Na Young Kim,Seohyun Kim,Sumin Cho,Gyumin Im,Byung Cheon Lee,Ji Hyung KimPubMed 36351405
- BMC cancer 22:5622022KDM3A-mediated SP1 activates PFKFB4 transcription to promote aerobic glycolysis in osteosarcoma and augment tumor development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang,Bin WangPubMed 35590288
- Technology in cancer research & treatment 19:15330338209458042020Suppressed "Warburg Effect" in Nasopharyngeal Carcinoma Via the Inhibition of Pyruvate Kinase Type M2-Mediated Energy Generation Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPenglong Zhao,Mengyan Zhou,Ruixiang Chen,Renjie SuPubMed 32815467
- Cancer research 80:675-6882020A Feedback Circuitry between Polycomb Signaling and Fructose-1, 6-Bisphosphatase Enables Hepatic and Renal Tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Liao,Shuye Deng,Liyan Xu,Wenfeng Pan,Shiyu Yang,Fufu Zheng,Xingui Wu,Hongrong Hu,Zhijun Liu,Junhang Luo,Rui Zhang,Dong-Ming Kuang,Jiajun Dong,Yi Wu,Hui Zhang,Penghui Zhou,Jin-Xin Bei,Yang Xu,Yin Ji,Peng Wang,Huai-Qiang Ju,Rui-Hua Xu,Bo LiPubMed 31948940
- Cell metabolism 31:174-188.e72019Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeiwei Huangyang,Fuming Li,Pearl Lee,Itzhak Nissim,Aalim M Weljie,Anthony Mancuso,Bo Li,Brian Keith,Sam S Yoon,M Celeste SimonPubMed 31761563
- Medical science monitor : international medical journal of experimental and clinical research 25:6023-60332019Knockdown of the Sonic Hedgehog (SHH) Gene Inhibits Proliferation of Hep3B and SMMC-7721 Hepatocellular Carcinoma Cells via the PI3K/Akt/PCK1 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouping Chen,Weifang ZhuPubMed 31406102
- Nature communications 9:49722018Interplay between hypoxia and androgen controls a metabolic switch conferring resistance to androgen/AR-targeted therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Geng,Changhui Xue,Janet Mendonca,Xiao-Xin Sun,Qiong Liu,Patrick N Reardon,Yingxiao Chen,Kendrick Qian,Vivian Hua,Alice Chen,Freddy Pan,Julia Yuan,Sang Dang,Tomasz M Beer,Mu-Shui Dai,Sushant K Kachhap,David Z QianPubMed 30478344
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 47:344-3552018Overexpression of PCK1 Gene Antagonizes Hepatocellular Carcinoma Through the Activation of Gluconeogenesis and Suppression of Glycolysis Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufu Tang,Yibing Zhang,Chunhui Wang,Zhongyi Sun,Longfei Li,Shuqun Cheng,Wenping ZhouPubMed 29768256
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Lysate, Cell Lysate
- Sensitivity
- = 1 µM
What's included?
Recommended products
Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples.
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Lysate, Cell Lysate
- Assay Platform
- Microplate reader
- Sensitivity
- = 1 µM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate (G6P) which is subsequently oxidized with the generation of a fluorescent product. This assay kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity 1 uM of F6P.
Other Notes
This product was previously called K689 Biovision PicoProbe Fructose-6-Phosphate Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fructose-6-phosphate also known as F6P is an important intermediate in the glycolysis and gluconeogenesis pathways. It has a molecular mass of approximately 260.1 g/mol. This metabolite is primarily expressed in cellular cytoplasm where it is involved in biochemical transformations necessary for energy production. Although it does not have specified protein complex associations fructose-6-phosphate remains an integral compound in metabolic processes.
- Biological function summary
Fructose-6-phosphate plays a significant role in carbohydrate metabolism. It serves as a substrate for numerous reactions including the conversion from glucose-6-phosphate to fructose-6-phosphate an important step catalyzed by the enzyme phosphoglucose isomerase. Fructose-6-phosphate's involvement in these reactions makes it essential for balancing energy through anabolic and catabolic activities.
- Pathways
Fructose-6-phosphate is highly involved in glycolysis and gluconeogenesis. In glycolysis it is further converted to fructose-16-bisphosphate a reaction facilitated by phosphofructokinase-1 (PFK-1) an enzyme that plays a critical role in regulating the pathway. Additionally in gluconeogenesis fructose-6-phosphate converts back to glucose-6-phosphate functioning in reverse to produce glucose from non-carbohydrate precursors.
- Associated diseases and disorders
Alterations in the metabolism of fructose-6-phosphate have been linked to diabetes and cancer. In diabetes abnormal regulation of glycolysis and gluconeogenesis can affect blood sugar levels where proteins like PFK-1 and phosphoglucose isomerase may play a part. Understanding these connections provides insight into developing therapies for managing metabolic disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (ab204720)
Typical F6P Standard calibration curve
Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (ab204720)
Typical F6P Standard calibration curve
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com