AB204720

Fructose-6-Phosphate Assay Kit (Fluorometric)

Name: Fructose-6-Phosphate Assay Kit (Fluorometric) (ab204720) | Abcam
Brand: Abcam Limited
SKU: AB204720
Price: 595 USD
Availability: InStock

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(8 Publications)

Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples.

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Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (AB204720)

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Supplier Data

Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (AB204720)

Fructose-1,6-Bisphosphate assay pathway

FuncS

Supplier Data

Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (AB204720)

Typical F6P Standard calibration curve

FuncS

Unknown

Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (AB204720)

Typical F6P Standard calibration curve

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell Lysate

Sensitivity

= 1 µM

Assay Platform

Microplate reader

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Product details

Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate (G6P) which is subsequently oxidized with the generation of a fluorescent product. This assay kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity 1 uM of F6P.

Other Notes
This product was previously called K689 Biovision PicoProbe Fructose-6-Phosphate Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fructose-6-phosphate also known as F6P is an important intermediate in the glycolysis and gluconeogenesis pathways. It has a molecular mass of approximately 260.1 g/mol. This metabolite is primarily expressed in cellular cytoplasm where it is involved in biochemical transformations necessary for energy production. Although it does not have specified protein complex associations fructose-6-phosphate remains an integral compound in metabolic processes.

Biological function summary

Fructose-6-phosphate plays a significant role in carbohydrate metabolism. It serves as a substrate for numerous reactions including the conversion from glucose-6-phosphate to fructose-6-phosphate an important step catalyzed by the enzyme phosphoglucose isomerase. Fructose-6-phosphate's involvement in these reactions makes it essential for balancing energy through anabolic and catabolic activities.

Pathways

Fructose-6-phosphate is highly involved in glycolysis and gluconeogenesis. In glycolysis it is further converted to fructose-16-bisphosphate a reaction facilitated by phosphofructokinase-1 (PFK-1) an enzyme that plays a critical role in regulating the pathway. Additionally in gluconeogenesis fructose-6-phosphate converts back to glucose-6-phosphate functioning in reverse to produce glucose from non-carbohydrate precursors.

Alterations in the metabolism of fructose-6-phosphate have been linked to diabetes and cancer. In diabetes abnormal regulation of glycolysis and gluconeogenesis can affect blood sugar levels where proteins like PFK-1 and phosphoglucose isomerase may play a part. Understanding these connections provides insight into developing therapies for managing metabolic disorders.

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Product protocols

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Target data

See full target information Fructose-6-phosphate

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111598 PubMed36351405

2022

MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Jung Yoo,Dong Wook Choi,Yeon Jin Roh,Yoon-Mi Lee,Ji-Hong Lim,Soohak Eo,Ho-Jae Lee,Na Young Kim,Seohyun Kim,Sumin Cho,Gyumin Im,Byung Cheon Lee,Ji Hyung Kim

BMC cancer 22:562 PubMed35590288

2022

KDM3A-mediated SP1 activates PFKFB4 transcription to promote aerobic glycolysis in osteosarcoma and augment tumor development.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Bin Wang

Technology in cancer research & treatment 19:1533033820945804 PubMed32815467

2020

Suppressed "Warburg Effect" in Nasopharyngeal Carcinoma Via the Inhibition of Pyruvate Kinase Type M2-Mediated Energy Generation Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Penglong Zhao,Mengyan Zhou,Ruixiang Chen,Renjie Su

Cancer research 80:675-688 PubMed31948940

2020

A Feedback Circuitry between Polycomb Signaling and Fructose-1, 6-Bisphosphatase Enables Hepatic and Renal Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Liao,Shuye Deng,Liyan Xu,Wenfeng Pan,Shiyu Yang,Fufu Zheng,Xingui Wu,Hongrong Hu,Zhijun Liu,Junhang Luo,Rui Zhang,Dong-Ming Kuang,Jiajun Dong,Yi Wu,Hui Zhang,Penghui Zhou,Jin-Xin Bei,Yang Xu,Yin Ji,Peng Wang,Huai-Qiang Ju,Rui-Hua Xu,Bo Li

Cell metabolism 31:174-188.e7 PubMed31761563

2019

Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Peiwei Huangyang,Fuming Li,Pearl Lee,Itzhak Nissim,Aalim M Weljie,Anthony Mancuso,Bo Li,Brian Keith,Sam S Yoon,M Celeste Simon

Medical science monitor : international medical journal of experimental and clinical research 25:6023-6033 PubMed31406102

2019

Knockdown of the Sonic Hedgehog (SHH) Gene Inhibits Proliferation of Hep3B and SMMC-7721 Hepatocellular Carcinoma Cells via the PI3K/Akt/PCK1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Youping Chen,Weifang Zhu

Nature communications 9:4972 PubMed30478344

2018

Interplay between hypoxia and androgen controls a metabolic switch conferring resistance to androgen/AR-targeted therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Geng,Changhui Xue,Janet Mendonca,Xiao-Xin Sun,Qiong Liu,Patrick N Reardon,Yingxiao Chen,Kendrick Qian,Vivian Hua,Alice Chen,Freddy Pan,Julia Yuan,Sang Dang,Tomasz M Beer,Mu-Shui Dai,Sushant K Kachhap,David Z Qian

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 47:344-355 PubMed29768256

2018

Overexpression of PCK1 Gene Antagonizes Hepatocellular Carcinoma Through the Activation of Gluconeogenesis and Suppression of Glycolysis Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yufu Tang,Yibing Zhang,Chunhui Wang,Zhongyi Sun,Longfei Li,Shuqun Cheng,Wenping Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Fructose-6-Phosphate Assay Kit (Fluorometric)

Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (AB204720)

Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (AB204720)

Functional Studies - Fructose-6-Phosphate Assay Kit (Fluorometric) (AB204720)

Key facts

Detection method

Sample types

Sensitivity

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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