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AB241022

Fructose Assay Kit (Fluorometric)

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(1 Publication)

Fructose Assay Kit (Fluorometric) (ab241022) measures free fructose that is enzymatically processed with the formation of a metabolite which reacts with the Probe to generate fluorescence (Ex/Em = 535/587 nm).
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Functional Studies - Fructose Assay Kit (Fluorometric) (AB241022)
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Supplier Data

Functional Studies - Fructose Assay Kit (Fluorometric) (AB241022)

Fructose Assay Kit reaction pathway

Functional Studies - Fructose Assay Kit (Fluorometric) (AB241022)
  • FuncS

Supplier Data

Functional Studies - Fructose Assay Kit (Fluorometric) (AB241022)

Example Fructose standard curve. Actual RFU values will vary with instrument.

Key facts

Detection method

Fluorescent

Sample types

Serum, Plasma, Cell Lysate, Tissue Homogenate, Urine

Results type

Quantitative

Sensitivity

= 0.005 nmol/well

Range

0.1 - 100 µmol/L

Assay time

1h 15m

Assay Platform

Microplate reader

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Product details

Fructose Assay Kit (Fluorometric) (ab241022) measures free fructose that is enzymatically processed with the formation of a metabolite which reacts with the Probe to generate fluorescence (Ex/Em = 535/587 nm). The kit provides a simple, highly sensitive, reliable method suitable for high throughput assay of D-fructose. Glucose interference can be removed by using the Sample Cleanup Mix. The kit can detect fructose in the range of 5 to 100 picomoles/well.

This product is manufactured by BioVision, an Abcam company and was previously called K611 PicoProbe™ Fructose Fluorometric Assay Kit. K611-100 is the same size as the 100 test size of ab241022.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241022-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Sample Cleanup Mix", "number":"AB241022-CMP06", "productcode":"" }, { "size":"1 x 400 µL", "name":"PicoProbe I", "number":"AB241022-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB241022-CMP01", "productcode":"AB323940" }, { "size":"1 x 1 Vial", "name":"Fructose Substrate Mix", "number":"AB241022-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Fructose Standard", "number":"AB241022-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fructose Enzyme Mix", "number":"AB241022-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix G", "number":"AB241022-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fructose often known as levulose is a simple sugar with a molecular mass of 180.16 g/mol. It is a monosaccharide found in many plants where it is frequently bonded with glucose to form the disaccharide sucrose. Fructose is absorbed directly into the bloodstream during digestion. Primarily expressed in the liver intestine and kidney fructose undergoes phosphorylation by fructokinase resulting in fructose-1-phosphate an essential step in fructose metabolism.
Biological function summary

Fructose serves as a fuel source and influences lipogenesis and insulin sensitivity. It does not operate as part of a complex but acts singularly in its metabolic functions. Fructose metabolism primarily handled by the liver impacts energy homeostasis and lipid synthesis with significant implications for glucose homeostasis. The fructose assay helps in quantifying fructose levels useful in both research and clinical settings to assess metabolic conditions.

Pathways

Fructose plays a significant role in the glycolytic and gluconeogenesis pathways. Fructose metabolism feeds into the glycolytic pathway where it gets converted to intermediates like dihydroxyacetone phosphate. This conversion connects fructose metabolism indirectly to gluconeogenesis. Additionally proteins such as hexokinase and aldolase B are integral to these processes facilitating the phosphorylation and cleavage necessary for further metabolic reactions.

Fructose relates closely to conditions like non-alcoholic fatty liver disease and metabolic syndrome. Excessive fructose metabolism can lead to lipid accumulation in the liver highlighting its link to non-alcoholic fatty liver disease. Metabolic syndrome characterized by insulin resistance and obesity often involves dysregulated fructose intake. Proteins like insulin and leptin interact intricately with fructose metabolism influencing these disorders and offering potential targets for therapeutic intervention.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 58:101451 PubMed35123128

2022

Crosstalk between TM4SF5 and GLUT8 regulates fructose metabolism in hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hyejin Lee,Eunmi Kim,Eun-Ae Shin,Jong Cheol Shon,Hyunseung Sun,Ji Eon Kim,Jae Woo Jung,Haesong Lee,Yangie Pinanga,Dae-Geun Song,Kwang-Hyeon Liu,Jung Weon Lee
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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