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AB196992

Fumarase Activity Assay Kit (Colorimetric)

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(7 Publications)

Fumarase Activity Assay kit (ab196992) provides a quick and easy method for monitoring fumarase activity in various samples.

View Alternative Names

Fumarase, HsFH, FH

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Functional Studies - Fumarase Activity Assay Kit (Colorimetric) (AB196992)
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Supplier Data

Functional Studies - Fumarase Activity Assay Kit (Colorimetric) (AB196992)

Relative Fumarase activity was calculated in mitochondria prepared from bovine heart (13.75 μg), mouse liver (17.62 μg), yeast (S. cerevisiae) (37.8 μg), and MCF-7 cells (16.8 μg).

Functional Studies - Fumarase Activity Assay Kit (Colorimetric) (AB196992)
  • FuncS

Supplier Data

Functional Studies - Fumarase Activity Assay Kit (Colorimetric) (AB196992)

Fumarase activity in mitochondria isolated from different sources.

Functional Studies - Fumarase Activity Assay Kit (Colorimetric) (AB196992)
  • FuncS

Supplier Data

Functional Studies - Fumarase Activity Assay Kit (Colorimetric) (AB196992)

NADH Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Fumarase Activity Assay kit (ab196992) provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450 nm.

The assay is simple and high-throughput ready and can detect less than 50 U/ml of fumarase activity in variety of sample types.

This product is manufactured by BioVision, an Abcam company and was previously called K596 Fumarase Activity Colorimetric Assay Kit. K596-100 is the same size as the 100 test size of ab196992.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab196992-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Fumarase Positive Control", "number":"AB196992-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB196992-CMP06", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"Fumarate Solution", "number":"AB196992-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Malate Enzyme Mix", "number":"AB196992-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB196992-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 64", "number":"AB196992-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fumarase sometimes referred to as fumarate hydratase is an enzyme involved in the Krebs cycle. Its mechanical function involves the reversible hydration/dehydration of fumarate to malate. The enzyme has a molecular mass around 50 kilodaltons. Fumarase expression occurs in the mitochondria of eukaryotic cells and it also appears in the cytosol contributing to the compartmentalization of its enzymatic activity.
Biological function summary

Fumarase plays a critical role in cellular respiration by facilitating the conversion of fumarate to malate within the tricarboxylic acid (TCA) cycle. This enzyme does not operate as part of a larger protein complex but its activity is essential for maintaining the flow of carbon through the TCA cycle which is integral to energy production. Its activity is upregulated when cells require increased ATP generation.

Pathways

Fumarase participates prominently in the TCA cycle a vital pathway for aerobic respiration where it interconverts fumarate and malate. The enzyme supports cellular energy production and this is closely linked with other TCA cycle enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase which further facilitate efficient energy conversion.

Fumarase deficiency leads to severe neurological impairment often manifesting as encephalopathy. This deficiency occurs due to mutations in the FH gene which encodes for fumarase. Additionally a reduction in fumarase activity associates with fumarase-related hereditary leiomyomatosis and renal cell cancer (HLRCC) linking fumarase with altered metabolic states in tumorigenesis. Dysregulation within this context often involves interactions with metabolically similar enzymes complicating the metabolic landscape and contributing to oncogenic processes.
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Product protocols

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Target data

Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed : 30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable).. Isoform Mitochondrial. Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH.. Isoform Cytoplasmic. Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ) (PubMed : 20231875, PubMed : 26237645). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs) : acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2) (PubMed : 26237645).
See full target information FH
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:553 PubMed37592347

2023

Rutaecarpine induces the differentiation of triple-negative breast cancer cells through inhibiting fumarate hydratase.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Lei,Yujia Pan,Rui Gao,Bin He,Zifeng Wang,Xinxing Lei,Zijian Zhang,Na Yang,Min Yan

Cell reports 40:111193 PubMed35977513

2022

Succinate uptake by T cells suppresses their effector function via inhibition of mitochondrial glucose oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy Gudgeon,Haydn Munford,Emma L Bishop,James Hill,Taylor Fulton-Ward,David Bending,Jennie Roberts,Daniel A Tennant,Sarah Dimeloe

Signal transduction and targeted therapy 6:375 PubMed34728602

2021

The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.

Applications

Unspecified application

Species

Unspecified reactive species

Xujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng Shang

Gastroenterology 161:1982-1997.e11 PubMed34425095

2021

Hepatic miR-144 Drives Fumarase Activity Preventing NRF2 Activation During Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Valerio Azzimato,Ping Chen,Emelie Barreby,Cecilia Morgantini,Laura Levi,Ana Vankova,Jennifer Jager,André Sulen,Marina Diotallevi,Joanne X Shen,Anne Miller,Ewa Ellis,Mikael Rydén,Erik Näslund,Anders Thorell,Volker M Lauschke,Keith M Channon,Mark J Crabtree,Arvand Haschemi,Siobhan M Craige,Mattia Mori,Francesco Spallotta,Myriam Aouadi

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33727417

2021

Monitoring tumor cell death in murine tumor models using deuterium magnetic resonance spectroscopy and spectroscopic imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Friederike Hesse,Vencel Somai,Felix Kreis,Flaviu Bulat,Alan J Wright,Kevin M Brindle

Theranostics 9:2424-2438 PubMed31131045

2019

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Shi,Min Zhou,Li Shang,Qianqian Du,Yueshuo Li,Longlong Xie,Xiaolan Liu,Min Tang,Xiangjian Luo,Jia Fan,Jian Zhou,Qiang Gao,ShuangJian Qiu,Weizhong Wu,Xin Zhang,Ann M Bode,Ya Cao

Cell death discovery 4:18 PubMed30062063

2018

Combined effects of (E404D) and (R409H) cause metabolic defects in primary cardiac malignant tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Zhou,Mengjia Xu,Weijia Zeng,Zhongzhong Chen,Guohui Lu,Yun Gong,Richard H Finnell,Huasheng Xiao,Bin Qiao,Hongyan Wang
View all publications
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