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AB102516

Fumarate Assay Kit

4

(1 Review)

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(19 Publications)

Fumarate Assay Kit ab102516 provides a convenient tool for sensitive detection of Fumarate in biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Fumarate Assay Kit (AB102516)
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Lab

Functional Studies - Fumarate Assay Kit (AB102516)

Fumarate measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

Functional Studies - Fumarate Assay Kit (AB102516)
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Supplier Data

Functional Studies - Fumarate Assay Kit (AB102516)

Fumurate Standard Curve

Functional Studies - Fumarate Assay Kit (AB102516)
  • FuncS

Lab

Functional Studies - Fumarate Assay Kit (AB102516)

Fumarate measured in cell lysates, background signal subtracted (duplicates +/- SD).

Functional Studies - Fumarate Assay Kit (AB102516)
  • FuncS

Lab

Functional Studies - Fumarate Assay Kit (AB102516)

Fumarate measured in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).

Functional Studies - Fumarate Assay Kit (AB102516)
  • FuncS

Lab

Functional Studies - Fumarate Assay Kit (AB102516)

Fumarate measured in biological fluids, background signal subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

> 1 nmol/well

Assay time

40m

Assay Platform

Microplate reader

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Product details

Fumarate Assay Kit ab102516 provides a convenient tool for sensitive detection of Fumarate in biological samples.

Fumarate assay principle
In the fumarate assay protocol, the fumarate enzyme mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (450 nm).

The assay can detect as low as 1 nmol of fumarate per well (20 uM).

Fumurate assay protocol summary
- add samples and standards to wells
- add reaction mix and incubate for 1 hr
- analyze with a microplate reader

Other Notes
This product was previously called K633 Biovision Fumarate Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fumarate also known as fumaric acid is an important intermediate in the citric acid cycle (Krebs cycle). This molecule has a relatively low molecular weight of about 116.07 g/mol. Fumarate is present in various tissues throughout the body particularly in cells with high metabolic activity where the citric acid cycle is active. As a component of cellular respiration fumarate plays an important role in energy production and metabolism facilitating the continuation of the cycle by converting succinate to malate through the enzyme succinate dehydrogenase.
Biological function summary

The role of fumarate extends beyond its mechanical conversion functions. It takes part in several cellular processes and is involved in redox reactions. It serves as a substrate for the enzyme fumarase which catalyzes the reversible hydration to malate further linking it with metabolic and redox equilibria. Additionally fumarate forms part of the succinate dehydrogenase complex which integrates into both the citric acid cycle and the electron transport chain having a dual function in energy production.

Pathways

Fumarate integrates within the interconnected web of the citric acid cycle and the electron transport chain. These pathways are important for oxidative phosphorylation where ATP production occurs. The fumarate transformation conducted by succinate dehydrogenase positions it within a protein complex pivotal for these pathways. This intertwined connection signifies an important relationship with other metabolic intermediates and proteins like NADH and ATP synthase which are necessary for cellular energy balance.

Fumarate has associations with conditions like fumarase deficiency and hereditary leiomyomatosis. Fumarase deficiency brings about severe neurological abnormalities due to disruptions in cellular energy metabolism. The relationship between fumarate and certain tumors involves its interaction with proteins such as fumarate hydratase where mutations can lead to abnormal accumulation and oncogenesis. Understanding fumarate's biochemical role may provide further insights into therapeutic pathways for these conditions.
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Product protocols

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Target data

See full target information Fumaric Acid
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:257 PubMed40189563

2025

Pharmacological targeting of the mitochondrial phosphatase PTPMT1 sensitizes hepatocellular carcinoma to ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Li,Yi Wang,Xinyan Li,Jiayi Xu,Liangwen Yan,Shenkang Tang,Chenyue Liu,Mengjiao Shi,Rongrong Liu,Yaping Zhao,Yi Zhang,Lan Yang,Yinggang Zhang,Gang Wang,Zongfang Li,Ying Guo,Yetong Feng,Pengfei Liu

Frontiers in oncology 12:912942 PubMed35847841

2022

The Molecular Role of HIF1α Is Elucidated in Chronic Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Singh,Ranjana Singh,Rashmi Kushwaha,Shailendra Prasad Verma,Anil Kumar Tripathi,Abbas Ali Mahdi

Frontiers in pharmacology 13:822743 PubMed35431970

2022

Effect of Metformin on Myocardial Injury Induced by Hepatic Ischemia-Reperfusion in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wen An,Ju-Seop Kang

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34551979

2021

Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ritesh K Aggarwal,Rebecca A Luchtel,Venkata Machha,Alexander Tischer,Yiyu Zou,Kith Pradhan,Nadia Ashai,Nandini Ramachandra,Joseph M Albanese,Jung-In Yang,Xiaoyang Wang,Srinivas Aluri,Shanisha Gordon,Ahmed Aboumohamed,Benjamin A Gartrell,Sassan Hafizi,James Pullman,Niraj Shenoy

Gastroenterology 161:1982-1997.e11 PubMed34425095

2021

Hepatic miR-144 Drives Fumarase Activity Preventing NRF2 Activation During Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Valerio Azzimato,Ping Chen,Emelie Barreby,Cecilia Morgantini,Laura Levi,Ana Vankova,Jennifer Jager,André Sulen,Marina Diotallevi,Joanne X Shen,Anne Miller,Ewa Ellis,Mikael Rydén,Erik Näslund,Anders Thorell,Volker M Lauschke,Keith M Channon,Mark J Crabtree,Arvand Haschemi,Siobhan M Craige,Mattia Mori,Francesco Spallotta,Myriam Aouadi

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2004993 PubMed34180138

2021

Low-Density Lipoprotein Receptor-Related Protein 6 Cell Surface Availability Regulates Fuel Metabolism in Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hei-Man Chow,Jacquelyne Ka-Li Sun,Ronald P Hart,Kenneth King-Yip Cheng,Clara H L Hung,Tsun-Ming Lau,Kin-Ming Kwan

Theranostics 11:4011-4029 PubMed33754045

2021

Adenylosuccinate lyase is oncogenic in colorectal cancer by causing mitochondrial dysfunction and independent activation of NRF2 and mTOR-MYC-axis.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Taha-Mehlitz,Gaia Bianco,Mairene Coto-Llerena,Venkatesh Kancherla,Glenn R Bantug,John Gallon,Caner Ercan,Federica Panebianco,Serenella Eppenberger-Castori,Marco von Strauss,Sebastian Staubli,Martin Bolli,Ralph Peterli,Matthias S Matter,Luigi M Terracciano,Markus von Flüe,Charlotte K Y Ng,Savas D Soysal,Otto Kollmar,Salvatore Piscuoglio

BMC cancer 20:470 PubMed32450839

2020

Pyruvate anaplerosis is a mechanism of resistance to pharmacological glutaminase inhibition in triple-receptor negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dean C Singleton,Anne-Lise Dechaume,Pamela M Murray,William P Katt,Bruce C Baguley,Euphemia Y Leung

Archives of biochemistry and biophysics 678:108167 PubMed31704098

2019

Fumarate accumulation involved in renal diabetic fibrosis in Goto-Kakizaki rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri Miura,Atsuko Hayakawa,Shohei Kikuchi,Hiroki Tsumoto,Keitaro Umezawa,Yuko Chiba,Yurie Soejima,Motoji Sawabe,Koji Fukui,Yoshihiro Akimoto,Tamao Endo

Theranostics 9:6631-6645 PubMed31588240

2019

Disruption of mitochondrial homeostasis with artemisinin unravels anti-angiogenesis effects via auto-paracrine mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan-Hao Tsui,Meng-Yu Wu,Li-Te Lin,Zhi-Hong Wen,Yi-Han Li,Pei-Yi Chu,Chia-Jung Li
View all publications
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