Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media.
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- Clinical pharmacokinetics 61:1039-10552022Liver Cirrhosis Affects the Pharmacokinetics of the Six Substrates of the Basel Phenotyping Cocktail Differently.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUrs Duthaler,Fabio Bachmann,Claudia Suenderhauf,Tanja Grandinetti,Florian Pfefferkorn,Manuel Haschke,Petr Hruz,Jamal Bouitbir,Stephan KrähenbühlPubMed 35570253
- Journal of nanobiotechnology 19:3632021A nanodrug system overexpressed circRNA_0001805 alleviates nonalcoholic fatty liver disease via miR-106a-5p/miR-320a and ABCA1/CPT1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Li,Jing Qi,Yishu Tang,Huaizheng Liu,Kefu Zhou,Zheren Dai,Lehong Yuan,Chuanzheng SunPubMed 34789275
- ACS synthetic biology 9:508-5162020Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihwan Chun et. al.PubMed 32119773
- Physiological genomics 52:203-2162020Hepatic proteome analysis reveals altered mitochondrial metabolism and suppressed acyl-CoA synthetase-1 in colon-26 tumor-induced cachexia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndy V Khamoui,Dorota Tokmina-Roszyk,Harry B Rossiter,Gregg B Fields,Nishant P VisavadiyaPubMed 32146873
- Animals : an open access journal from MDPI 6:2016Intermittent Suckling in Combination with an Older Weaning Age Improves Growth, Feed Intake and Aspects of Gastrointestinal Tract Carbohydrate Absorption in Pigs after Weaning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana L Turpin,Pieter Langendijk,Tai-Yuan Chen,John R PluskePubMed 27792138
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Assay time
40m
- Sensitivity
< 0.007 nmol/well
Target data
What's included?
Recommended products
Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
< 0.007 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media. In this assay, oxidation of galactose generates a product that reacts with the galactose probe to produce color (OD570nm) or fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.
High concentrations of lactose could result in elevated readings for free galactose. Galactose oxidase is not completely selective, and it can react with the galactose moiety of lactose.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K621 Galactose Colorimetric/Fluorometric Assay Kit. K621-100 is the same size as the 100 test size of ab83382.
Galactose (C6H12O6 MW: 180.16 g/mol) is one of the major naturally occurring sugars, a monosaccharide that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Often present as an anomeric mixture of α-D-galactose and β-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galactose a simple sugar with a molecular weight of 180.16 g/mol is a monosaccharide similar to glucose. It serves as an energy source and forms part of glycoproteins and glycolipids. In the human body the liver ovaries and seminal vesicles express galactose. Cells in these tissues utilize galactose for various cellular activities contributing to functions like cell-cell recognition and signaling. Common assays measure galactose concentrations to understand metabolic conditions and tools like galactose assay kits offer specific insights.
- Biological function summary
Galactose plays a critical role in the Leloir pathway converting galactose into glucose-1-phosphate. This conversion involves several enzymes including galactokinase and uridylyltransferase. Not existing within any larger complex galactose activity supports the proper metabolism of carbohydrates and the synthesis of complex biomolecules. Its function ensures proper cellular metabolism by providing precursors for energy production and biosynthesis.
- Pathways
Galactose contributes significantly to the glycolysis and gluconeogenesis processes. UDP-galactose an activated form of galactose interacts in these metabolic pathways highlighting its role in carbohydrate metabolism. The protein galactokinase is essential in phosphorylating galactose for its subsequent incorporation into these pathways. Another protein galactose-1-phosphate uridylyltransferase further catalyzes the transformation of galactose derivatives showing the interconnectedness of these proteins within metabolic networks.
- Associated diseases and disorders
Defective galactose metabolism leads to conditions like galactosemia a genetic disorder affecting an enzyme in the Leloir pathway. Classical galactosemia involves mutations in the galactose-1-phosphate uridylyltransferase gene causing an accumulation of galactose-1-phosphate in tissues and organs leading to toxicity. A related condition congenital cataracts can arise from galactose pathway disruptions where crystallins proteins in the lens of the eye are affected by elevated galactitol resulting from excess galactose. Understanding galactose's biological role helps monitor and manage these disorders effectively.
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5 product images
Functional Studies - Galactose Assay Kit (ab83382)
Galactose measured in tissue lysates showing quantity (nmol) per mg of extracted protein.
Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were tested undiluted.
Functional Studies - Galactose Assay Kit (ab83382)
Galactose measured in cell lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 4e6 cells/mL were used. Samples were tested undiluted.
Functional Studies - Galactose Assay Kit (ab83382)
Galactose levels measured in biological fluids showing quantity (nmol) per mL. Samples tested undiluted.
Functional Studies - Galactose Assay Kit (ab83382)
Galactose Standard Curve – Fluorometric mode.
Example of Fluorometric galactose standard curve obtained with ab83382 in fluorometric mode.
Functional Studies - Galactose Assay Kit (ab83382)
Galactose Standard Curve – Colorimetric mode.
Example of galactose standard curve obtained with ab83382 in colorimetric mode.
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