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AB241029

Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric)

5

(1 Review)

|

(4 Publications)

Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (ab241029) uses a simple method with measurement of release of pNA from a GGT enzyme substrate. Readout on any colorimetric (418 nm) plate reader.

- includes GGT enzyme positive control and pNA standard for quantitation
3 Images
Functional Studies - Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (AB241029)
  • FuncS

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Functional Studies - Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (AB241029)

GGT positive control and serum sample.

Functional Studies - Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (AB241029)
  • FuncS

Unknown

Functional Studies - Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (AB241029)

pNA standard curve.

Schematic Diagram - Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (AB241029)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (AB241029)

Representative image of Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) ab241029

Components shown from left to right :

- GGT Positive Control

- pNA Standard II

- Assay Buffer 57

- GGT Substrate

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell Lysate, Serum

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Gamma Glutamyl Transferase (GGT) Assay Kit (Colorimetric) (ab241029) provides a convenient tool for sensitive detection of GGT activity in a variety of samples.

GGT assay principle
In the GGT assay, GGT in the sample acts on L γ Glutamyl pNA, releasing pNA. The level of pNA can be quantified colorimetrically (418 nm).

GGT assay protocol summary
- add samples and standards to wells
- add substrate mix
- analyze standard wells on plate reader
- incubate for 3-min at 37°C
- analyze on plate reader, incubate for another 30-min to 2-hrs at 37°C and then analyze again on plate reader
(or preferably, analyze in kinetic mode at 37°C for 30-min to 2-hrs)

This assay detects GGT activity as low as 0.5 mIU.

Other Notes
This product is manufactured by BioVision, an Abcam company and was previously called K784 Gamma Glutamyl Transferase (GGT) Activity Colorimetric Assay Kit. K784-100 is the same size as the 100 test size of ab241029.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241029-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"GGT Substrate", "number":"AB241029-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"GGT Positive Control", "number":"AB241029-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 57", "number":"AB241029-CMP01", "productcode":"" }, { "size":"1 x 400 µL", "name":"pNA Standard II", "number":"AB241029-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Transglutaminase 1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107015 PubMed37360686

2023

A salivary biomarker panel to detect liver cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas Trevisan França de Lima,Darrell H G Crawford,Daniel A Broszczak,Xi Zhang,Kim Bridle R,Chamindie Punyadeera

Frontiers in pharmacology 13:893336 PubMed35774609

2022

Exploring Ganweikang Tablet as a Candidate Drug for NAFLD Through Network Pharmacology Analysis and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanrui Ma,Xinyu Wang,Jing Zhang,Yun Zhao,Yunqing Hua,Chao Zhang,Guobin Zheng,Guangyan Yang,Jianli Guan,Huahuan Li,Meng Li,Lin Kang,Jiaqing Xiang,Guanwei Fan,Shu Yang

British journal of pharmacology 178:2443-2460 PubMed33661551

2021

Tectorigenin alleviates intrahepatic cholestasis by inhibiting hepatic inflammation and bile accumulation via activation of PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqing Xiang,Guangyan Yang,Chuanrui Ma,Lingling Wei,Han Wu,Wei Zhang,Xiuhua Tao,Lingyun Jiang,Zhen Liang,Lin Kang,Shu Yang

Animal science journal = Nihon chikusan Gakkaiho 91:e13434 PubMed32696560

2020

Sidr honey abrogates the oxidative stress and downregulates the hyaluronic acid concentration and gene expression of TGF-β1 and COL1a1 in rat model of thioacetamide-induced hepatic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed M Zeweil,Kadry M Sadek,Mohamed F Elsadek,Sahar F Mahmoud,Badreldin M Ahmed,Asmaa F Khafaga
View all publications
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